Nitric oxide is an obligate bacterial nitrification intermediate produced by hydroxylamine oxidoreductase
Ammonia (NH )-oxidizing bacteria (AOB) emit substantial amounts of nitric oxide (NO) and nitrous oxide (N O), both of which contribute to the harmful environmental side effects of large-scale agriculture. The currently accepted model for AOB metabolism involves NH oxidation to nitrite (NO ) via a si...
Gespeichert in:
| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS Jg. 114; H. 31; S. 8217 |
|---|---|
| Hauptverfasser: | , |
| Format: | Journal Article |
| Sprache: | Englisch |
| Veröffentlicht: |
United States
01.08.2017
|
| Schlagworte: | |
| ISSN: | 1091-6490, 1091-6490 |
| Online-Zugang: | Weitere Angaben |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| Zusammenfassung: | Ammonia (NH
)-oxidizing bacteria (AOB) emit substantial amounts of nitric oxide (NO) and nitrous oxide (N
O), both of which contribute to the harmful environmental side effects of large-scale agriculture. The currently accepted model for AOB metabolism involves NH
oxidation to nitrite (NO
) via a single obligate intermediate, hydroxylamine (NH
OH). Within this model, the multiheme enzyme hydroxylamine oxidoreductase (HAO) catalyzes the four-electron oxidation of NH
OH to NO
We provide evidence that HAO oxidizes NH
OH by only three electrons to NO under both anaerobic and aerobic conditions. NO
observed in HAO activity assays is a nonenzymatic product resulting from the oxidation of NO by O
under aerobic conditions. Our present study implies that aerobic NH
oxidation by AOB occurs via two obligate intermediates, NH
OH and NO, necessitating a mediator of the third enzymatic step. |
|---|---|
| Bibliographie: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 1091-6490 1091-6490 |
| DOI: | 10.1073/pnas.1704504114 |