Directed evolution of adenine base editors with increased activity and therapeutic application

The foundational adenine base editors (for example, ABE7.10) enable programmable A•T to G•C point mutations but editing efficiencies can be low at challenging loci in primary human cells. Here we further evolve ABE7.10 using a library of adenosine deaminase variants to create ABE8s. At NGG protospac...

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Veröffentlicht in:	Nature biotechnology Jg. 38; H. 7; S. 892 - 900
Hauptverfasser:	Gaudelli, Nicole M, Lam, Dieter K, Rees, Holly A, Solá-Esteves, Noris M, Barrera, Luis A, Born, David A, Edwards, Aaron, Gehrke, Jason M, Lee, Seung-Joo, Liquori, Alexander J, Murray, Ryan, Packer, Michael S, Rinaldi, Conrad, Slaymaker, Ian M, Yen, Jonathan, Young, Lauren E, Ciaramella, Giuseppe
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States Nature Publishing Group 01.07.2020
Schlagworte:	Adenine Adenine - metabolism Adenosine Adenosine Deaminase CD34 antigen CRISPR-Cas Systems - genetics Cytosine - metabolism Deamination Deoxyribonucleic acid Directed evolution DNA DNA - genetics Editing Evolution Fetuses Gene Editing - methods HEK293 Cells Hemoglobin Humans Loci Lymphocytes Lymphocytes T mRNA Mutation Mutation - genetics Ribonucleic acid RNA RNA, Guide, CRISPR-Cas Systems
ISSN:	1087-0156, 1546-1696, 1546-1696
Online-Zugang:	Volltext
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