High‐resolution plant metabolomics: from mass spectral features to metabolites and from whole‐cell analysis to subcellular metabolite distributions

The main goal of metabolomics is the comprehensive qualitative and quantitative analysis of the time‐ and space‐resolved distribution of all metabolites present in a given biological system. Because metabolite structures, in contrast to transcript and protein sequences, are not directly deducible fr...

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Bibliographic Details
Published in:The Plant journal : for cell and molecular biology Vol. 70; no. 1; pp. 39 - 50
Main Authors: Kueger, Stephan, Steinhauser, Dirk, Willmitzer, Lothar, Giavalisco, Patrick
Format: Journal Article
Language:English
Published: Oxford, UK Blackwell Publishing Ltd 01.04.2012
Blackwell
Subjects:
amino acid sequences
analytical methods
Biological and medical sciences
Cell Fractionation
Cellular biology
Fundamental and applied biological sciences. Psychology
Isotope Labeling
Mass Spectrometry
Mass Spectrometry - methods
metabolism
metabolite annotation
Metabolites
metabolomics
Metabolomics - methods
methods
non‐aqueous fractionation
nucleotide sequences
Plant biology
Plant physiology and development
Plants
Plants - metabolism
quantitative analysis
Spectrum analysis
subcellular localization
systems biology
Metabolomics
Fractionation
High resolution
Vegetals
Metabolite
subcellular localization
Annotation
Biological system
metabolite annotation
Plant
Systems biology
Distribution
Cell
Mass spectrometry
non-aqueous fractionation
ISSN:0960-7412, 1365-313X, 1365-313X
Online Access:Get full text
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Description
Summary:The main goal of metabolomics is the comprehensive qualitative and quantitative analysis of the time‐ and space‐resolved distribution of all metabolites present in a given biological system. Because metabolite structures, in contrast to transcript and protein sequences, are not directly deducible from the genomic DNA sequence, the massive increase in genomic information is only indirectly of use to metabolomics, leaving compound annotation as a key problem to be solved by the available analytical techniques. Furthermore, as metabolites vary widely in both concentration and chemical behavior, there is no single analytical procedure allowing the unbiased and comprehensive structural elucidation and determination of all metabolites present in a given biological system. In this review the different approaches for targeted and non‐targeted metabolomics analysis will be described with special emphasis on mass spectrometry‐based techniques. Particular attention is given to approaches which can be employed for the annotation of unknown compounds. In the second part, the different experimental approaches aimed at tissue‐specific or subcellular analysis of metabolites are discussed including a range of non‐mass spectrometry based technologies.
Bibliography:http://dx.doi.org/10.1111/j.1365-313X.2012.04902.x
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ISSN:0960-7412
1365-313X
1365-313X
DOI:10.1111/j.1365-313X.2012.04902.x