Overexpression of cyclooxygenase-2 in non-small cell lung cancer

Evidence is accumulating to suggest that the inducible isoenzyme of cyclooxygenase (COX)-2 is up-regulated in human cancers and epidemiological studies indicate that COX inhibitors may have a protective effect on the development of lung cancer. We used immunohistochemistry and Western blotting to in...

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Vydané v:Respiratory medicine Ročník 98; číslo 2; s. 164 - 172
Hlavní autori: Petkova, D.K., Clelland, C., Ronan, J., Pang, L., Coulson, J.M., Lewis, S., Knox, A.J.
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Oxford Elsevier Ltd 01.02.2004
Elsevier
Elsevier Limited
Predmet:
Adenocarcinoma - enzymology
Aged
Biological and medical sciences
Blotting, Western
Carcinoma, Non-Small-Cell Lung - enzymology
Carcinoma, Small Cell - enzymology
Colorectal cancer
Cyclooxygenase
Cyclooxygenase 2
Female
Humans
Immunohistochemistry
Isoenzymes - metabolism
Lung cancer
Lung cancer cell lines
Lung Neoplasms - enzymology
Male
Medical research
Medical sciences
Membrane Proteins
Middle Aged
Mortality
Neoplasm Proteins - metabolism
Pneumology
Prostaglandin-Endoperoxide Synthases - metabolism
Studies
Tumor Cells, Cultured
Tumors
Tumors of the respiratory system and mediastinum
Immunohistochemistry
Lung cancer cell lines
Cyclooxygenase
Lung cancer
Lung disease
Prostaglandin-endoperoxide synthase
Respiratory disease
Enzyme
Cyclooxygenase 2
Malignant tumor
non-small cell lung carcinoma
Bronchopulmonary malignant tumor
Anatomic pathology
Cell line
Bronchus disease
Oxidoreductases
Tumor cell
Non small cell carcinoma
Pneumology
ISSN:0954-6111, 1532-3064
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Shrnutí:Evidence is accumulating to suggest that the inducible isoenzyme of cyclooxygenase (COX)-2 is up-regulated in human cancers and epidemiological studies indicate that COX inhibitors may have a protective effect on the development of lung cancer. We used immunohistochemistry and Western blotting to investigate COX expression in lung tumour specimens and three lung cancer cell lines. Sixty-five archival lung tissue samples, including 46 squamous cell and 6 adenocarcinoma lung resections, and 13 small cell lung cancer (SCLC) biopsies were studied. Dense and intense cytoplasmic COX-2 staining was found in all 52 resections from non-small cell lung cancer (NSCLC). The staining was diffuse and much stronger than adjacent respiratory epithelium. COX-2 staining was relatively weak in the majority of the SCLC samples. The bronchial and bronchiolar epithelium in the surrounding normal lung structures showed uniform COX immunoreactivity with apical concentration of the stain. There was no increase in COX-1 staining in any tumour type. Western blot analysis of the cancer lines revealed significantly higher expression of COX-1 in CORL23 line and COX-2 in two NSCLC cell lines (MOR/P; A549) compared with the expression of COX-1 and COX-2 in cultured normal bronchial epithelial cells. Our findings demonstrated COX-2 overexpression in NSCLC.
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ISSN:0954-6111
1532-3064
DOI:10.1016/j.rmed.2003.09.006