PFA fixation enables artifact-free super-resolution imaging of the actin cytoskeleton and associated proteins

Super-resolution microscopy (SRM) allows precise localization of proteins in cellular organelles and structures, including the actin cytoskeleton. Yet sample preparation protocols for SRM are rather anecdotal and still being optimized. Thus, SRM-based imaging of the actin cytoskeleton and associated...

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Bibliographic Details
Published in:	Biology open Vol. 5; no. 7; pp. 1001 - 1009
Main Authors:	Leyton-Puig, Daniela, Kedziora, Katarzyna M., Isogai, Tadamoto, van den Broek, Bram, Jalink, Kees, Innocenti, Metello
Format:	Journal Article
Language:	English
Published:	England The Company of Biologists Ltd 15.07.2016 The Company of Biologists
Subjects:	Actin Actin cytoskeleton Cellular structure Clathrin Cytoskeleton dSTORM Fixation Image resolution Lamellipodia Localization Methods & Techniques Organelles Protein localization Proteins Pseudopodia Regulatory proteins Reproducibility Sample preparation Super-resolution microscopy (SRM) Protein localization dSTORM Fixation Actin cytoskeleton Super-resolution microscopy (SRM)
ISSN:	2046-6390, 2046-6390
Online Access:	Get full text
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