Proinflammatory cytokines and their membrane-bound receptors are altered in the lymphocytes of schizophrenia patients

Abnormalities of protein levels of proinflammatory cytokines and their soluble receptors have been reported in the plasma/serum of schizophrenia (SZ) patients. To examine if SZ is also associated with the abnormal gene expression of cytokines and their membrane-bound receptors, we studied mRNA expre...

Full description

Saved in:
Bibliographic Details
Published in:Schizophrenia research Vol. 164; no. 1-3; pp. 193 - 198
Main Authors: Pandey, Ghanshyam N., Ren, Xinguo, Rizavi, Hooriyah S., Zhang, Hui
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01.05.2015
Subjects:
Adult
Cytokines - genetics
Cytokines - metabolism
Enzyme-Linked Immunosorbent Assay
Female
Gp130
Humans
IL-1R1
IL-1R2
IL-1RA
IL-1β
IL-6
IL-6R
Lymphocytes
Lymphocytes - metabolism
Male
Middle Aged
Proinflammatory cytokines
Psychiatric Status Rating Scales
Psychiatry
Receptors, Cytokine - genetics
Receptors, Cytokine - metabolism
RNA, Messenger - metabolism
Schizophrenia
Schizophrenia - blood
Schizophrenia - pathology
TNF-α
TNFR1
TNFR2
Young Adult
IL-6R
IL-1RA
Schizophrenia
TNFR2
IL-1β
IL-6
TNFR1
IL-1R2
IL-1R1
TNF-α
Lymphocytes
Gp130
Proinflammatory cytokines
ISSN:0920-9964, 1573-2509, 1573-2509
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abnormalities of protein levels of proinflammatory cytokines and their soluble receptors have been reported in the plasma/serum of schizophrenia (SZ) patients. To examine if SZ is also associated with the abnormal gene expression of cytokines and their membrane-bound receptors, we studied mRNA expression of proinflammatory cytokines and their receptors in lymphocytes of SZ patients and normal control (NC) subjects. We determined the protein and mRNA expression of proinflammatory cytokines and mRNA expression of their receptors in lymphocytes from 30 SZ patients and 30 drug-free NC subjects. The subjects were diagnosed according to DSM-IV criteria. Protein levels of cytokines were determined by ELISA, and mRNA levels in lymphocytes were determined by the qPCR method. We found that the mRNA levels of IL-6, TNF-α, IL-1R1, TNFR1, and TNFR2, but not IL-1β, IL-1R2, IL-1RA, IL-6R, or GP130 were significantly increased in lymphocytes of SZ patients compared with NC subjects. We also found that the protein expression of IL-6 and TNF-α, but not IL-1β, was also significantly increased in SZ patients compared with NC subjects. These studies suggest that in addition to the reported abnormalities of proinflammatory cytokines and their soluble receptors in the plasma of SZ patients, an abnormal gene expression of these cytokines and their membrane-bound receptors may be involved in the pathogenesis of SZ.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0920-9964
1573-2509
1573-2509
DOI:10.1016/j.schres.2015.02.004