Assessing sensitivity and reproducibility of RT-ddPCR and RT-qPCR for the quantification of SARS-CoV-2 in wastewater
[Display omitted] •Wastewater surveillance methods captured the emergence of SARS-CoV-2 presence in a community.•In a direct comparison of workflows, RT-ddPCR demonstrated superior analytical sensitivity.•RT-ddPCR was resistant to inhibition in a wastewater matrix and was reproducible across laborat...
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| Veröffentlicht in: | Journal of virological methods Jg. 297; S. 114230 |
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| Format: | Journal Article |
| Sprache: | Englisch |
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Elsevier B.V
01.11.2021
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| ISSN: | 0166-0934, 1879-0984, 1879-0984 |
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| Abstract | [Display omitted]
•Wastewater surveillance methods captured the emergence of SARS-CoV-2 presence in a community.•In a direct comparison of workflows, RT-ddPCR demonstrated superior analytical sensitivity.•RT-ddPCR was resistant to inhibition in a wastewater matrix and was reproducible across laboratories.
Throughout the COVID-19 global pandemic there has been significant interest and investment in using Wastewater-Based Epidemiology (WBE) for surveillance of viral pathogen presence and infections at the community level. There has been a push for widescale implementation of standardized protocols to quantify viral loads in a range of wastewater systems. To address concerns regarding sensitivity, limits of quantification, and large-scale reproducibility, a comparison of two similar workflows using RT-qPCR and RT-ddPCR was conducted. Sixty raw wastewater influent samples were acquired from nine distinct wastewater treatment plants (WWTP’s) served by the Hampton Roads Sanitation District (HRSD, Virginia Beach, Virginia) over a 6-month period beginning March 9th, 2020. Common reagents, controls, master mixes and nucleic acid extracts were shared between two individual processing groups based out of HRSD and the UNC Chapel Hill Institute of Marine Sciences (IMS, Morehead City, North Carolina). Samples were analyzed in parallel using One-Step RT-qPCR and One-Step RT-ddPCR with Nucleocapsid Protein 2 (N2) specific primers and probe. Influent SARS-CoV-2 N2 concentrations steadily increased over time spanning a range from non-detectable to 2.13E + 05 copies/L. Systematic dilution of the extracts indicated that inhibitory components in the wastewater matrices did not significantly impede the detection of a positive N2 signal for either workflow. The RT-ddPCR workflow had a greater analytical sensitivity with a lower Limit of Detection (LOD) at 0.066 copies/μl of template compared to RT-qPCR with a calculated LOD of 12.0 copies/μL of template. Interlaboratory comparisons using non-parametric correlation analysis demonstrated that there was a strong, significant, positive correlation between split extracts when employing RT-ddPCR for analysis with a ρ value of 0.86. |
|---|---|
| AbstractList | Throughout the COVID-19 global pandemic there has been significant interest and investment in using Wastewater-Based Epidemiology (WBE) for surveillance of viral pathogen presence and infections at the community level. There has been a push for widescale implementation of standardized protocols to quantify viral loads in a range of wastewater systems. To address concerns regarding sensitivity, limits of quantification, and large-scale reproducibility, a comparison of two similar workflows using RT-qPCR and RT-ddPCR was conducted. Sixty raw wastewater influent samples were acquired from nine distinct wastewater treatment plants (WWTP's) served by the Hampton Roads Sanitation District (HRSD, Virginia Beach, Virginia) over a 6-month period beginning March 9th, 2020. Common reagents, controls, master mixes and nucleic acid extracts were shared between two individual processing groups based out of HRSD and the UNC Chapel Hill Institute of Marine Sciences (IMS, Morehead City, North Carolina). Samples were analyzed in parallel using One-Step RT-qPCR and One-Step RT-ddPCR with Nucleocapsid Protein 2 (N2) specific primers and probe. Influent SARS-CoV-2 N2 concentrations steadily increased over time spanning a range from non-detectable to 2.13E + 05 copies/L. Systematic dilution of the extracts indicated that inhibitory components in the wastewater matrices did not significantly impede the detection of a positive N2 signal for either workflow. The RT-ddPCR workflow had a greater analytical sensitivity with a lower Limit of Detection (LOD) at 0.066 copies/μl of template compared to RT-qPCR with a calculated LOD of 12.0 copies/μL of template. Interlaboratory comparisons using non-parametric correlation analysis demonstrated that there was a strong, significant, positive correlation between split extracts when employing RT-ddPCR for analysis with a ρ value of 0.86. Throughout the COVID-19 global pandemic there has been significant interest and investment in using Wastewater-Based Epidemiology (WBE) for surveillance of viral pathogen presence and infections at the community level. There has been a push for widescale implementation of standardized protocols to quantify viral loads in a range of wastewater systems. To address concerns regarding sensitivity, limits of quantification, and large-scale reproducibility, a comparison of two similar workflows using RT-qPCR and RT-ddPCR was conducted. Sixty raw wastewater influent samples were acquired from nine distinct wastewater treatment plants (WWTP's) served by the Hampton Roads Sanitation District (HRSD, Virginia Beach, Virginia) over a 6-month period beginning March 9th, 2020. Common reagents, controls, master mixes and nucleic acid extracts were shared between two individual processing groups based out of HRSD and the UNC Chapel Hill Institute of Marine Sciences (IMS, Morehead City, North Carolina). Samples were analyzed in parallel using One-Step RT-qPCR and One-Step RT-ddPCR with Nucleocapsid Protein 2 (N2) specific primers and probe. Influent SARS-CoV-2 N2 concentrations steadily increased over time spanning a range from non-detectable to 2.13E + 05 copies/L. Systematic dilution of the extracts indicated that inhibitory components in the wastewater matrices did not significantly impede the detection of a positive N2 signal for either workflow. The RT-ddPCR workflow had a greater analytical sensitivity with a lower Limit of Detection (LOD) at 0.066 copies/μl of template compared to RT-qPCR with a calculated LOD of 12.0 copies/μL of template. Interlaboratory comparisons using non-parametric correlation analysis demonstrated that there was a strong, significant, positive correlation between split extracts when employing RT-ddPCR for analysis with a ρ value of 0.86.Throughout the COVID-19 global pandemic there has been significant interest and investment in using Wastewater-Based Epidemiology (WBE) for surveillance of viral pathogen presence and infections at the community level. There has been a push for widescale implementation of standardized protocols to quantify viral loads in a range of wastewater systems. To address concerns regarding sensitivity, limits of quantification, and large-scale reproducibility, a comparison of two similar workflows using RT-qPCR and RT-ddPCR was conducted. Sixty raw wastewater influent samples were acquired from nine distinct wastewater treatment plants (WWTP's) served by the Hampton Roads Sanitation District (HRSD, Virginia Beach, Virginia) over a 6-month period beginning March 9th, 2020. Common reagents, controls, master mixes and nucleic acid extracts were shared between two individual processing groups based out of HRSD and the UNC Chapel Hill Institute of Marine Sciences (IMS, Morehead City, North Carolina). Samples were analyzed in parallel using One-Step RT-qPCR and One-Step RT-ddPCR with Nucleocapsid Protein 2 (N2) specific primers and probe. Influent SARS-CoV-2 N2 concentrations steadily increased over time spanning a range from non-detectable to 2.13E + 05 copies/L. Systematic dilution of the extracts indicated that inhibitory components in the wastewater matrices did not significantly impede the detection of a positive N2 signal for either workflow. The RT-ddPCR workflow had a greater analytical sensitivity with a lower Limit of Detection (LOD) at 0.066 copies/μl of template compared to RT-qPCR with a calculated LOD of 12.0 copies/μL of template. Interlaboratory comparisons using non-parametric correlation analysis demonstrated that there was a strong, significant, positive correlation between split extracts when employing RT-ddPCR for analysis with a ρ value of 0.86. [Display omitted] •Wastewater surveillance methods captured the emergence of SARS-CoV-2 presence in a community.•In a direct comparison of workflows, RT-ddPCR demonstrated superior analytical sensitivity.•RT-ddPCR was resistant to inhibition in a wastewater matrix and was reproducible across laboratories. Throughout the COVID-19 global pandemic there has been significant interest and investment in using Wastewater-Based Epidemiology (WBE) for surveillance of viral pathogen presence and infections at the community level. There has been a push for widescale implementation of standardized protocols to quantify viral loads in a range of wastewater systems. To address concerns regarding sensitivity, limits of quantification, and large-scale reproducibility, a comparison of two similar workflows using RT-qPCR and RT-ddPCR was conducted. Sixty raw wastewater influent samples were acquired from nine distinct wastewater treatment plants (WWTP’s) served by the Hampton Roads Sanitation District (HRSD, Virginia Beach, Virginia) over a 6-month period beginning March 9th, 2020. Common reagents, controls, master mixes and nucleic acid extracts were shared between two individual processing groups based out of HRSD and the UNC Chapel Hill Institute of Marine Sciences (IMS, Morehead City, North Carolina). Samples were analyzed in parallel using One-Step RT-qPCR and One-Step RT-ddPCR with Nucleocapsid Protein 2 (N2) specific primers and probe. Influent SARS-CoV-2 N2 concentrations steadily increased over time spanning a range from non-detectable to 2.13E + 05 copies/L. Systematic dilution of the extracts indicated that inhibitory components in the wastewater matrices did not significantly impede the detection of a positive N2 signal for either workflow. The RT-ddPCR workflow had a greater analytical sensitivity with a lower Limit of Detection (LOD) at 0.066 copies/μl of template compared to RT-qPCR with a calculated LOD of 12.0 copies/μL of template. Interlaboratory comparisons using non-parametric correlation analysis demonstrated that there was a strong, significant, positive correlation between split extracts when employing RT-ddPCR for analysis with a ρ value of 0.86. |
| ArticleNumber | 114230 |
| Author | Noble, Rachel Thompson, Hannah Blackwood, Denene Larson, Allison Ciesielski, Mark Clerkin, Thomas Gonzalez, Raul |
| Author_xml | – sequence: 1 givenname: Mark orcidid: 0000-0002-8996-2323 surname: Ciesielski fullname: Ciesielski, Mark organization: Institute of Marine Sciences, University of North Carolina at Chapel Hill, Morehead City, North Carolina, United States – sequence: 2 givenname: Denene surname: Blackwood fullname: Blackwood, Denene organization: Institute of Marine Sciences, University of North Carolina at Chapel Hill, Morehead City, North Carolina, United States – sequence: 3 givenname: Thomas surname: Clerkin fullname: Clerkin, Thomas organization: Institute of Marine Sciences, University of North Carolina at Chapel Hill, Morehead City, North Carolina, United States – sequence: 4 givenname: Raul orcidid: 0000-0002-8115-7709 surname: Gonzalez fullname: Gonzalez, Raul organization: Hampton Roads Sanitation District, Virginia Beach, Virginia, United States – sequence: 5 givenname: Hannah orcidid: 0000-0001-5456-5291 surname: Thompson fullname: Thompson, Hannah organization: Hampton Roads Sanitation District, Virginia Beach, Virginia, United States – sequence: 6 givenname: Allison surname: Larson fullname: Larson, Allison organization: Hampton Roads Sanitation District, Virginia Beach, Virginia, United States – sequence: 7 givenname: Rachel surname: Noble fullname: Noble, Rachel email: rtnoble@email.unc.edu organization: Institute of Marine Sciences, University of North Carolina at Chapel Hill, Morehead City, North Carolina, United States |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/34252511$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1056/NEJMcibr2032888 10.1056/NEJMc2009324 10.1001/jama.2020.2565 10.3892/ijmm.2020.4673 10.1016/j.scitotenv.2020.139298 10.1016/S2666-5247(20)30172-5 10.1128/JCM.02620-12 10.1053/j.gastro.2020.02.055 10.1002/cpmc.62 10.1016/j.coesh.2020.09.003 10.3201/eid2606.200301 10.1111/j.1365-2672.2012.05305.x 10.1016/j.envint.2020.105689 10.1016/j.watres.2020.116296 10.1016/j.watres.2014.12.008 10.3201/eid2608.201246 10.1016/S2468-1253(20)30087-X 10.1038/s41591-020-0817-4 10.1016/j.scitotenv.2020.140444 10.1016/j.bbrc.2020.04.136 10.1128/AEM.01430-12 10.1093/clinchem/hvaa125 10.1016/j.watres.2009.07.016 10.1038/s41598-020-80715-1 10.1373/clinchem.2013.206375 10.1016/j.bdq.2016.08.002 10.1016/j.jviromet.2008.05.016 10.11613/BM.2012.031 10.1016/j.watres.2012.09.056 10.1093/infdis/jiu384 10.1016/j.scitotenv.2020.141750 10.1016/j.scitotenv.2020.138764 10.1373/clinchem.2008.112797 10.1016/j.watres.2013.08.024 10.1590/S0074-02762009000400007 10.3389/fmars.2018.00133 10.1016/j.scitotenv.2020.139076 10.1016/S0140-6736(20)30183-5 10.1016/j.tjem.2018.08.001 10.2307/2347973 10.1016/j.ebiom.2016.01.008 10.1038/s41598-017-02217-x |
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| Keywords | SARS-CoV-2 Wastewater Molecular methods PCR |
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| References | Wu, Xiao, Zhang, Moniz, Endo, Armas (bib0245) 2020 Staley, Gordon, Schoen, Harwood (bib0220) 2012; 78 Bai, Yao, Wei, Tian, Jin, Chen, Wang (bib0040) 2020; 323 Loos, Carvalho, Antonio, Comero, Locoro, Tavazzi (bib0150) 2013; 47 Zeng, Liu, Ma, Zhao, Yang, Liu (bib0265) 2020; 527 Foladori, Cutrupi, Segata, Manara, Pinto, Malpei (bib0100) 2020; 743 Cao, Griffith, Dorevitch, Weisberg (bib0060) 2012; 113 Kitajima, Ahmed, Bibby, Carducci, Gerba, Hamilton (bib0140) 2020; 739 Xu, Li, Zhu, Liang, Fang, Gong (bib0255) 2020; 26 Deprez, Corbisier, Kortekaas, Mazoua, Beaz Hidalgo, Trapmann, Emons (bib0085) 2016; 9 Westhaus, Weber, Schiwy, Linnemann, Brinkmann, Widera (bib0240) 2021; 751 Ahmed, Bivins, Bertsch, Bibby, Choi, Farkas (bib0010) 2020; 17 Gonzalez, Curtis, Bivins, Bibby, Weir, Yetka (bib0110) 2020; 186 Ahmed, Angel, Edson, Bibby, Bivins, O’Brien (bib0005) 2020; 728 Bustin, Benes, Garson, Hellemans, Huggett, Kubista (bib0055) 2009; 55 Akoglu (bib0020) 2018; 18 Ahmed, Simpson, Bertsch, Bibby, Bivins, Blackall (bib0015) 2021 Feng, Roguet, McClary-Gutierrez, Newton, Kloczko, Meiman, McLellan (bib0095) 2021 Xiao, Tang, Zheng, Liu, Li, Shan (bib0250) 2020; 158 Hayden, Gu, Ingersoll, Abdul-Ali, Shi, Pounds, Caliendo (bib0115) 2013; 51 Medema, Heijnen, Elsinga, Italiaander, Brouwer (bib0165) 2020 Public Health England (bib0185) 2021 Royston (bib0205) 1982; 31 Baric (bib0050) 2020; 383 Decaro, Elia, Campolo, Desario, Mari, Radogna (bib0080) 2008; 151 McHugh (bib0160) 2012; 22 Asghar, Diop, Weldegebriel, Malik, Shetty, El Bassioni (bib0035) 2014; 210 Falzone, Musso, Gattuso, Bongiorno, Palermo, Scalia (bib0090) 2020; 46 Arnaout, Lee, Lee, Callahan, Yen, Smith (bib0030) 2020 Cevik, Tate, Lloyd, Maraolo, Schafers, Ho (bib0075) 2021; 2 Anderson, Maldarelli (bib0025) 2018; 51 Huang, Wang, Li, Ren, Zhao, Hu (bib0120) 2020; 395 Huggett, Foy, Benes, Emslie, Garson, Haynes (bib0125) 2013; 59 Kassambara (bib0135) 2018 Yong (bib0260) 2020 Meselson (bib0170) 2020; 382 Gibas, Lambirth, Mittal, Juel, Barua, Brazell (bib0105) 2021 Lu, Wang, Sakthivel, Whitaker, Murray, Kamili (bib0155) 2020; 26 Obermeier, Muehlhans, Hoppe, Karsch, Tief, Seeber (bib0175) 2016; 4 Reemtsma, Miehe, Duennbier, Jekel (bib0200) 2010; 44 Orive, Lertxundi, Barcelo (bib0180) 2020; 732 R Core Team (bib0190) 2020 Taylor, Laperriere, Germain (bib0230) 2017; 7 Cao, Sivaganesan, Kinzelman, Blackwood, Noble, Haugland (bib0065) 2013; 47 Sims, Kasprzyk-Hordern (bib0215) 2020; 139 Cao, Raith, Griffith (bib0070) 2015; 70 Randazzo, Cuevas-Ferrando, Sanjuán, Domingo-Calap, Sánchez (bib0195) 2020 Vasudevan, Xu, Servellita, Miller, Liu, Gopez (bib0235) 2021; 11 Lodder, De Roda Husman (bib0145) 2020; 5 Baker, Steel, Nieukirk, Klinck (bib0045) 2018; 5 Tang, Tong, Wang, Dai, Li, Liu (bib0225) 2020; 26 Schlindwein, Simões, Barardi (bib0210) 2009; 104 Huggett, Whale, De Spiegelaere, Trypsteen, Nour, Bae (bib0130) 2020; 66 Bai (10.1016/j.jviromet.2021.114230_bib0040) 2020; 323 Orive (10.1016/j.jviromet.2021.114230_bib0180) 2020; 732 Huggett (10.1016/j.jviromet.2021.114230_bib0130) 2020; 66 Cao (10.1016/j.jviromet.2021.114230_bib0060) 2012; 113 Westhaus (10.1016/j.jviromet.2021.114230_bib0240) 2021; 751 Arnaout (10.1016/j.jviromet.2021.114230_bib0030) 2020 Huggett (10.1016/j.jviromet.2021.114230_bib0125) 2013; 59 Ahmed (10.1016/j.jviromet.2021.114230_bib0005) 2020; 728 Meselson (10.1016/j.jviromet.2021.114230_bib0170) 2020; 382 Tang (10.1016/j.jviromet.2021.114230_bib0225) 2020; 26 Staley (10.1016/j.jviromet.2021.114230_bib0220) 2012; 78 Xu (10.1016/j.jviromet.2021.114230_bib0255) 2020; 26 Reemtsma (10.1016/j.jviromet.2021.114230_bib0200) 2010; 44 Cao (10.1016/j.jviromet.2021.114230_bib0070) 2015; 70 Wu (10.1016/j.jviromet.2021.114230_bib0245) 2020 Akoglu (10.1016/j.jviromet.2021.114230_bib0020) 2018; 18 Deprez (10.1016/j.jviromet.2021.114230_bib0085) 2016; 9 Taylor (10.1016/j.jviromet.2021.114230_bib0230) 2017; 7 Loos (10.1016/j.jviromet.2021.114230_bib0150) 2013; 47 Foladori (10.1016/j.jviromet.2021.114230_bib0100) 2020; 743 Zeng (10.1016/j.jviromet.2021.114230_bib0265) 2020; 527 Baker (10.1016/j.jviromet.2021.114230_bib0045) 2018; 5 Xiao (10.1016/j.jviromet.2021.114230_bib0250) 2020; 158 Decaro (10.1016/j.jviromet.2021.114230_bib0080) 2008; 151 Kitajima (10.1016/j.jviromet.2021.114230_bib0140) 2020; 739 Yong (10.1016/j.jviromet.2021.114230_bib0260) 2020 Gonzalez (10.1016/j.jviromet.2021.114230_bib0110) 2020; 186 Lodder (10.1016/j.jviromet.2021.114230_bib0145) 2020; 5 Baric (10.1016/j.jviromet.2021.114230_bib0050) 2020; 383 Ahmed (10.1016/j.jviromet.2021.114230_bib0015) 2021 Schlindwein (10.1016/j.jviromet.2021.114230_bib0210) 2009; 104 Lu (10.1016/j.jviromet.2021.114230_bib0155) 2020; 26 Vasudevan (10.1016/j.jviromet.2021.114230_bib0235) 2021; 11 Bustin (10.1016/j.jviromet.2021.114230_bib0055) 2009; 55 Cao (10.1016/j.jviromet.2021.114230_bib0065) 2013; 47 Obermeier (10.1016/j.jviromet.2021.114230_bib0175) 2016; 4 Feng (10.1016/j.jviromet.2021.114230_bib0095) 2021 Asghar (10.1016/j.jviromet.2021.114230_bib0035) 2014; 210 Medema (10.1016/j.jviromet.2021.114230_bib0165) 2020 Ahmed (10.1016/j.jviromet.2021.114230_bib0010) 2020; 17 Huang (10.1016/j.jviromet.2021.114230_bib0120) 2020; 395 Hayden (10.1016/j.jviromet.2021.114230_bib0115) 2013; 51 Sims (10.1016/j.jviromet.2021.114230_bib0215) 2020; 139 Falzone (10.1016/j.jviromet.2021.114230_bib0090) 2020; 46 Public Health England (10.1016/j.jviromet.2021.114230_bib0185) 2021 Anderson (10.1016/j.jviromet.2021.114230_bib0025) 2018; 51 Royston (10.1016/j.jviromet.2021.114230_bib0205) 1982; 31 Cevik (10.1016/j.jviromet.2021.114230_bib0075) 2021; 2 McHugh (10.1016/j.jviromet.2021.114230_bib0160) 2012; 22 Randazzo (10.1016/j.jviromet.2021.114230_bib0195) 2020 R Core Team (10.1016/j.jviromet.2021.114230_bib0190) 2020 Gibas (10.1016/j.jviromet.2021.114230_bib0105) 2021 Kassambara (10.1016/j.jviromet.2021.114230_bib0135) 2018 |
| References_xml | – volume: 728 start-page: 138764 year: 2020 ident: bib0005 article-title: First confirmed detection of SARS-CoV-2 in untreated wastewater in Australia: a proof of concept for the wastewater surveillance of COVID-19 in the community publication-title: Sci. Total Environ. – volume: 22 start-page: 276 year: 2012 end-page: 282 ident: bib0160 article-title: Interrater reliability: the kappa statistic publication-title: Biochem. Med. (Zagreb) – volume: 139 start-page: 105689 year: 2020 ident: bib0215 article-title: Future perspectives of wastewater-based epidemiology: monitoring infectious disease spread and resistance to the community level publication-title: Environ. Int. – volume: 739 start-page: 139076 year: 2020 ident: bib0140 article-title: SARS-CoV-2 in wastewater: state of the knowledge and research needs publication-title: Sci. Total Environ. – volume: 26 start-page: 1337 year: 2020 end-page: 1339 ident: bib0225 article-title: Detection of novel coronavirus by RT-PCR in stool specimen from asymptomatic child, China publication-title: Emerg. Infect. Dis. – volume: 395 start-page: 497 year: 2020 end-page: 506 ident: bib0120 article-title: Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China publication-title: Lancet – volume: 9 start-page: 29 year: 2016 end-page: 39 ident: bib0085 article-title: Validation of a digital PCR method for quantification of DNA copy number concentrations by using a certified reference material publication-title: Biomol. Detect. Quantif. – year: 2020 ident: bib0260 article-title: NEA Monitoring Wastewater in Bid to Give Dorms the Virus All-Clear – volume: 31 start-page: 115 year: 1982 ident: bib0205 article-title: An extension of shapiro and Wilk’s W test for normality to large samples publication-title: Appl. Stat. – volume: 11 year: 2021 ident: bib0235 article-title: Digital droplet PCR accurately quantifies SARS-CoV-2 viral load from crude lysate without nucleic acid purification publication-title: Sci. Rep. – volume: 66 start-page: 1012 year: 2020 end-page: 1029 ident: bib0130 article-title: The digital MIQE guidelines update: minimum information for publication of quantitative digital PCR experiments for 2020 publication-title: Clin. Chem. – volume: 44 start-page: 596 year: 2010 end-page: 604 ident: bib0200 article-title: Polar pollutants in municipal wastewater and the water cycle: occurrence and removal of benzotriazoles publication-title: Water Res. – year: 2020 ident: bib0165 article-title: Presence of SARS-Coronavirus-2 in Sewage – volume: 527 start-page: 618 year: 2020 end-page: 623 ident: bib0265 article-title: Biochemical characterization of SARS-CoV-2 nucleocapsid protein publication-title: Biochem. Biophys. Res. Commun. – year: 2018 ident: bib0135 article-title: Ggpubr:’ ggplot2’ Based Publication Ready Plots. R Package Version 0.2 – volume: 5 start-page: 533 year: 2020 end-page: 534 ident: bib0145 article-title: SARS-CoV-2 in wastewater: potential health risk, but also data source publication-title: Lancet Gastroenterol. Hepatol. – volume: 383 start-page: 2684 year: 2020 end-page: 2686 ident: bib0050 article-title: Emergence of a highly fit SARS-CoV-2 variant publication-title: N. Engl. J. Med. – year: 2021 ident: bib0105 article-title: Implementing building-level SARS-CoV-2 wastewater surveillance on a university campus publication-title: medRxiv – volume: 751 start-page: 141750 year: 2021 ident: bib0240 article-title: Detection of SARS-CoV-2 in raw and treated wastewater in Germany – suitability for COVID-19 surveillance and potential transmission risks publication-title: Sci. Total Environ. – volume: 26 start-page: 502 year: 2020 end-page: 505 ident: bib0255 article-title: Characteristics of pediatric SARS-CoV-2 infection and potential evidence for persistent fecal viral shedding publication-title: Nat. Med. – volume: 70 start-page: 337 year: 2015 end-page: 349 ident: bib0070 article-title: Droplet digital PCR for simultaneous quantification of general and human-associated fecal indicators for water quality assessment publication-title: Water Res. – volume: 18 start-page: 91 year: 2018 end-page: 93 ident: bib0020 article-title: User’s guide to correlation coefficients publication-title: Turk. J. Emerg. Med. – volume: 26 year: 2020 ident: bib0155 article-title: US CDC real-time reverse transcription PCR panel for detection of severe acute respiratory syndrome coronavirus 2 publication-title: Emerg. Infect. Dis. – volume: 732 start-page: 139298 year: 2020 ident: bib0180 article-title: Early SARS-CoV-2 outbreak detection by sewage-based epidemiology publication-title: Sci. Total Environ. – volume: 47 start-page: 6475 year: 2013 end-page: 6487 ident: bib0150 article-title: EU-wide monitoring survey on emerging polar organic contaminants in wastewater treatment plant effluents publication-title: Water Res. – volume: 59 start-page: 892 year: 2013 end-page: 902 ident: bib0125 article-title: The digital MIQE guidelines: minimum information for publication of quantitative digital PCR experiments publication-title: Clin. Chem. – volume: 104 start-page: 576 year: 2009 end-page: 579 ident: bib0210 article-title: Comparative study of two extraction methods for enteric virus recovery from sewage sludge by molecular methods publication-title: Memórias do Instituto Oswaldo Cruz – volume: 382 year: 2020 ident: bib0170 article-title: Droplets and aerosols in the transmission of SARS-CoV-2 publication-title: N. Engl. J. Med. – volume: 47 start-page: 233 year: 2013 end-page: 241. ident: bib0065 article-title: Effect of platform, reference material, and quantification model on enumeration of Enterococcus by quantitative PCR methods publication-title: Water Res. – volume: 210 start-page: S294 year: 2014 end-page: S303 ident: bib0035 article-title: Environmental surveillance for polioviruses in the global polio eradication initiative publication-title: J. Infect. Dis. – year: 2021 ident: bib0095 article-title: Evaluation of Sampling Frequency and Normalization of SARS-CoV-2 Wastewater Concentrations for Capturing COVID-19 Burdens in the Community – year: 2020 ident: bib0030 article-title: SARS-CoV2 testing: the limit of detection matters publication-title: bioRxiv – volume: 158 start-page: 1831 year: 2020 end-page: 1833 ident: bib0250 article-title: Evidence for gastrointestinal infection of SARS-CoV-2 publication-title: Gastroenterology – volume: 17 start-page: 82 year: 2020 end-page: 93 ident: bib0010 article-title: Surveillance of SARS-CoV-2 RNA in wastewater: methods optimisation and quality control are crucial for generating reliable public health information publication-title: Curr. Opin. Environ. Sci. Health – year: 2020 ident: bib0190 article-title: R: A Language and Environment for Statistical Computing – volume: 46 start-page: 957 year: 2020 end-page: 9964. ident: bib0090 article-title: Sensitivity assessment of droplet digital PCR for SARS-CoV-2 detection publication-title: Int. J. Mol. Med. – year: 2020 ident: bib0245 article-title: SARS-CoV-2 titers in wastewater foreshadow dynamics and clinical presentation of new COVID-19 cases publication-title: medRxiv – year: 2021 ident: bib0015 article-title: Minimizing Errors in RT-PCR Detection and Quantification of SARS-CoV-2 RNA for Wastewater Surveillance – year: 2021 ident: bib0185 article-title: Investigation of novel SARS-CoV-2 variant: Variant of Concern 202012/01. Technical Brief 3 (Publication No. GW-1856) – volume: 151 start-page: 167 year: 2008 end-page: 171 ident: bib0080 article-title: Detection of bovine coronavirus using a TaqMan-based real-time RT-PCR assay publication-title: J. Virol. Methods – volume: 55 start-page: 611 year: 2009 end-page: 6622 ident: bib0055 article-title: The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments publication-title: Clin. Chem. – volume: 323 start-page: 1406 year: 2020 ident: bib0040 article-title: Presumed asymptomatic carrier transmission of COVID-19 publication-title: JAMA – volume: 186 start-page: 116296 year: 2020 ident: bib0110 article-title: COVID-19 surveillance in Southeastern Virginia using wastewater-based epidemiology publication-title: Water Res. – volume: 78 start-page: 7317 year: 2012 end-page: 7326 ident: bib0220 article-title: Performance of two quantitative PCR methods for microbial source tracking of human sewage and implications for microbial risk assessment in recreational waters publication-title: Appl. Environ. Microbiol. – volume: 51 start-page: 540 year: 2013 end-page: 546 ident: bib0115 article-title: Comparison of droplet digital PCR to real-time PCR for quantitative detection of cytomegalovirus publication-title: J. Clin. Microbiol. – volume: 51 start-page: e62 year: 2018 ident: bib0025 article-title: Quantification of HIV DNA using droplet digital PCR techniques publication-title: Curr. Protoc. Microbiol. – volume: 7 start-page: 2409 year: 2017 ident: bib0230 article-title: Droplet Digital PCR versus qPCR for gene expression analysis with low abundant targets: from variable nonsense to publication quality data publication-title: Sci. Rep. – volume: 2 start-page: e13 year: 2021 end-page: e22 ident: bib0075 article-title: SARS-CoV-2, SARS-CoV, and MERS-CoV viral load dynamics, duration of viral shedding, and infectiousness: a systematic review and meta-analysis publication-title: Lancet Microbe – volume: 743 start-page: 140444 year: 2020 ident: bib0100 article-title: SARS-CoV-2 from faeces to wastewater treatment: what do we know? A review publication-title: Sci. Total Environ. – volume: 113 start-page: 66 year: 2012 end-page: 75 ident: bib0060 article-title: Effectiveness of qPCR permutations, internal controls and dilution as means for minimizing the impact of inhibition while measuring Enterococcus in environmental waters publication-title: J. Appl. Microbiol. – year: 2020 ident: bib0195 article-title: Metropolitan wastewater analysis for COVID-19 epidemiological surveillance publication-title: medRxiv – volume: 5 year: 2018 ident: bib0045 article-title: Environmental DNA (eDNA) from the wake of the whales: droplet digital PCR for detection and species identification publication-title: Front. Mar. Sci. – volume: 4 start-page: 191 year: 2016 end-page: 196 ident: bib0175 article-title: Enabling precision medicine with digital case classification at the point-of-care publication-title: EBioMedicine – volume: 383 start-page: 2684 issue: 27 year: 2020 ident: 10.1016/j.jviromet.2021.114230_bib0050 article-title: Emergence of a highly fit SARS-CoV-2 variant publication-title: N. Engl. J. Med. doi: 10.1056/NEJMcibr2032888 – volume: 382 issue: 21 year: 2020 ident: 10.1016/j.jviromet.2021.114230_bib0170 article-title: Droplets and aerosols in the transmission of SARS-CoV-2 publication-title: N. Engl. J. Med. doi: 10.1056/NEJMc2009324 – year: 2020 ident: 10.1016/j.jviromet.2021.114230_bib0190 – volume: 323 start-page: 1406 issue: 14 year: 2020 ident: 10.1016/j.jviromet.2021.114230_bib0040 article-title: Presumed asymptomatic carrier transmission of COVID-19 publication-title: JAMA doi: 10.1001/jama.2020.2565 – volume: 46 start-page: 957 issue: 3 year: 2020 ident: 10.1016/j.jviromet.2021.114230_bib0090 article-title: Sensitivity assessment of droplet digital PCR for SARS-CoV-2 detection publication-title: Int. J. Mol. Med. doi: 10.3892/ijmm.2020.4673 – volume: 732 start-page: 139298 year: 2020 ident: 10.1016/j.jviromet.2021.114230_bib0180 article-title: Early SARS-CoV-2 outbreak detection by sewage-based epidemiology publication-title: Sci. Total Environ. doi: 10.1016/j.scitotenv.2020.139298 – volume: 2 start-page: e13 issue: 1 year: 2021 ident: 10.1016/j.jviromet.2021.114230_bib0075 article-title: SARS-CoV-2, SARS-CoV, and MERS-CoV viral load dynamics, duration of viral shedding, and infectiousness: a systematic review and meta-analysis publication-title: Lancet Microbe doi: 10.1016/S2666-5247(20)30172-5 – year: 2021 ident: 10.1016/j.jviromet.2021.114230_bib0185 – volume: 51 start-page: 540 issue: 2 year: 2013 ident: 10.1016/j.jviromet.2021.114230_bib0115 article-title: Comparison of droplet digital PCR to real-time PCR for quantitative detection of cytomegalovirus publication-title: J. Clin. Microbiol. doi: 10.1128/JCM.02620-12 – year: 2018 ident: 10.1016/j.jviromet.2021.114230_bib0135 – year: 2020 ident: 10.1016/j.jviromet.2021.114230_bib0245 article-title: SARS-CoV-2 titers in wastewater foreshadow dynamics and clinical presentation of new COVID-19 cases – year: 2020 ident: 10.1016/j.jviromet.2021.114230_bib0030 article-title: SARS-CoV2 testing: the limit of detection matters – volume: 158 start-page: 1831 issue: 6 year: 2020 ident: 10.1016/j.jviromet.2021.114230_bib0250 article-title: Evidence for gastrointestinal infection of SARS-CoV-2 publication-title: Gastroenterology doi: 10.1053/j.gastro.2020.02.055 – volume: 51 start-page: e62 issue: 1 year: 2018 ident: 10.1016/j.jviromet.2021.114230_bib0025 article-title: Quantification of HIV DNA using droplet digital PCR techniques publication-title: Curr. Protoc. Microbiol. doi: 10.1002/cpmc.62 – volume: 17 start-page: 82 year: 2020 ident: 10.1016/j.jviromet.2021.114230_bib0010 article-title: Surveillance of SARS-CoV-2 RNA in wastewater: methods optimisation and quality control are crucial for generating reliable public health information publication-title: Curr. Opin. Environ. Sci. Health doi: 10.1016/j.coesh.2020.09.003 – volume: 26 start-page: 1337 issue: 6 year: 2020 ident: 10.1016/j.jviromet.2021.114230_bib0225 article-title: Detection of novel coronavirus by RT-PCR in stool specimen from asymptomatic child, China publication-title: Emerg. Infect. Dis. doi: 10.3201/eid2606.200301 – volume: 113 start-page: 66 issue: 1 year: 2012 ident: 10.1016/j.jviromet.2021.114230_bib0060 article-title: Effectiveness of qPCR permutations, internal controls and dilution as means for minimizing the impact of inhibition while measuring Enterococcus in environmental waters publication-title: J. Appl. Microbiol. doi: 10.1111/j.1365-2672.2012.05305.x – volume: 139 start-page: 105689 year: 2020 ident: 10.1016/j.jviromet.2021.114230_bib0215 article-title: Future perspectives of wastewater-based epidemiology: monitoring infectious disease spread and resistance to the community level publication-title: Environ. Int. doi: 10.1016/j.envint.2020.105689 – volume: 186 start-page: 116296 year: 2020 ident: 10.1016/j.jviromet.2021.114230_bib0110 article-title: COVID-19 surveillance in Southeastern Virginia using wastewater-based epidemiology publication-title: Water Res. doi: 10.1016/j.watres.2020.116296 – volume: 70 start-page: 337 year: 2015 ident: 10.1016/j.jviromet.2021.114230_bib0070 article-title: Droplet digital PCR for simultaneous quantification of general and human-associated fecal indicators for water quality assessment publication-title: Water Res. doi: 10.1016/j.watres.2014.12.008 – volume: 26 issue: 8 year: 2020 ident: 10.1016/j.jviromet.2021.114230_bib0155 article-title: US CDC real-time reverse transcription PCR panel for detection of severe acute respiratory syndrome coronavirus 2 publication-title: Emerg. Infect. Dis. doi: 10.3201/eid2608.201246 – volume: 5 start-page: 533 issue: 6 year: 2020 ident: 10.1016/j.jviromet.2021.114230_bib0145 article-title: SARS-CoV-2 in wastewater: potential health risk, but also data source publication-title: Lancet Gastroenterol. Hepatol. doi: 10.1016/S2468-1253(20)30087-X – volume: 26 start-page: 502 issue: 4 year: 2020 ident: 10.1016/j.jviromet.2021.114230_bib0255 article-title: Characteristics of pediatric SARS-CoV-2 infection and potential evidence for persistent fecal viral shedding publication-title: Nat. Med. doi: 10.1038/s41591-020-0817-4 – volume: 743 start-page: 140444 year: 2020 ident: 10.1016/j.jviromet.2021.114230_bib0100 article-title: SARS-CoV-2 from faeces to wastewater treatment: what do we know? A review publication-title: Sci. Total Environ. doi: 10.1016/j.scitotenv.2020.140444 – year: 2020 ident: 10.1016/j.jviromet.2021.114230_bib0260 – volume: 527 start-page: 618 issue: 3 year: 2020 ident: 10.1016/j.jviromet.2021.114230_bib0265 article-title: Biochemical characterization of SARS-CoV-2 nucleocapsid protein publication-title: Biochem. Biophys. Res. Commun. doi: 10.1016/j.bbrc.2020.04.136 – volume: 78 start-page: 7317 issue: 20 year: 2012 ident: 10.1016/j.jviromet.2021.114230_bib0220 article-title: Performance of two quantitative PCR methods for microbial source tracking of human sewage and implications for microbial risk assessment in recreational waters publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.01430-12 – year: 2021 ident: 10.1016/j.jviromet.2021.114230_bib0095 – volume: 66 start-page: 1012 issue: 8 year: 2020 ident: 10.1016/j.jviromet.2021.114230_bib0130 article-title: The digital MIQE guidelines update: minimum information for publication of quantitative digital PCR experiments for 2020 publication-title: Clin. Chem. doi: 10.1093/clinchem/hvaa125 – volume: 44 start-page: 596 issue: 2 year: 2010 ident: 10.1016/j.jviromet.2021.114230_bib0200 article-title: Polar pollutants in municipal wastewater and the water cycle: occurrence and removal of benzotriazoles publication-title: Water Res. doi: 10.1016/j.watres.2009.07.016 – volume: 11 issue: 1 year: 2021 ident: 10.1016/j.jviromet.2021.114230_bib0235 article-title: Digital droplet PCR accurately quantifies SARS-CoV-2 viral load from crude lysate without nucleic acid purification publication-title: Sci. Rep. doi: 10.1038/s41598-020-80715-1 – volume: 59 start-page: 892 issue: 6 year: 2013 ident: 10.1016/j.jviromet.2021.114230_bib0125 article-title: The digital MIQE guidelines: minimum information for publication of quantitative digital PCR experiments publication-title: Clin. Chem. doi: 10.1373/clinchem.2013.206375 – volume: 9 start-page: 29 year: 2016 ident: 10.1016/j.jviromet.2021.114230_bib0085 article-title: Validation of a digital PCR method for quantification of DNA copy number concentrations by using a certified reference material publication-title: Biomol. Detect. Quantif. doi: 10.1016/j.bdq.2016.08.002 – volume: 151 start-page: 167 issue: 2 year: 2008 ident: 10.1016/j.jviromet.2021.114230_bib0080 article-title: Detection of bovine coronavirus using a TaqMan-based real-time RT-PCR assay publication-title: J. Virol. Methods doi: 10.1016/j.jviromet.2008.05.016 – volume: 22 start-page: 276 issue: 3 year: 2012 ident: 10.1016/j.jviromet.2021.114230_bib0160 article-title: Interrater reliability: the kappa statistic publication-title: Biochem. Med. (Zagreb) doi: 10.11613/BM.2012.031 – volume: 47 start-page: 233 issue: 1 year: 2013 ident: 10.1016/j.jviromet.2021.114230_bib0065 article-title: Effect of platform, reference material, and quantification model on enumeration of Enterococcus by quantitative PCR methods publication-title: Water Res. doi: 10.1016/j.watres.2012.09.056 – volume: 210 start-page: S294 issue: suppl 1 year: 2014 ident: 10.1016/j.jviromet.2021.114230_bib0035 article-title: Environmental surveillance for polioviruses in the global polio eradication initiative publication-title: J. Infect. Dis. doi: 10.1093/infdis/jiu384 – volume: 751 start-page: 141750 year: 2021 ident: 10.1016/j.jviromet.2021.114230_bib0240 article-title: Detection of SARS-CoV-2 in raw and treated wastewater in Germany – suitability for COVID-19 surveillance and potential transmission risks publication-title: Sci. Total Environ. doi: 10.1016/j.scitotenv.2020.141750 – year: 2021 ident: 10.1016/j.jviromet.2021.114230_bib0015 – volume: 728 start-page: 138764 year: 2020 ident: 10.1016/j.jviromet.2021.114230_bib0005 article-title: First confirmed detection of SARS-CoV-2 in untreated wastewater in Australia: a proof of concept for the wastewater surveillance of COVID-19 in the community publication-title: Sci. Total Environ. doi: 10.1016/j.scitotenv.2020.138764 – volume: 55 start-page: 611 issue: 4 year: 2009 ident: 10.1016/j.jviromet.2021.114230_bib0055 article-title: The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments publication-title: Clin. Chem. doi: 10.1373/clinchem.2008.112797 – volume: 47 start-page: 6475 issue: 17 year: 2013 ident: 10.1016/j.jviromet.2021.114230_bib0150 article-title: EU-wide monitoring survey on emerging polar organic contaminants in wastewater treatment plant effluents publication-title: Water Res. doi: 10.1016/j.watres.2013.08.024 – volume: 104 start-page: 576 issue: 4 year: 2009 ident: 10.1016/j.jviromet.2021.114230_bib0210 article-title: Comparative study of two extraction methods for enteric virus recovery from sewage sludge by molecular methods publication-title: Memórias do Instituto Oswaldo Cruz doi: 10.1590/S0074-02762009000400007 – volume: 5 year: 2018 ident: 10.1016/j.jviromet.2021.114230_bib0045 article-title: Environmental DNA (eDNA) from the wake of the whales: droplet digital PCR for detection and species identification publication-title: Front. Mar. Sci. doi: 10.3389/fmars.2018.00133 – volume: 739 start-page: 139076 year: 2020 ident: 10.1016/j.jviromet.2021.114230_bib0140 article-title: SARS-CoV-2 in wastewater: state of the knowledge and research needs publication-title: Sci. Total Environ. doi: 10.1016/j.scitotenv.2020.139076 – year: 2020 ident: 10.1016/j.jviromet.2021.114230_bib0165 – volume: 395 start-page: 497 issue: 10223 year: 2020 ident: 10.1016/j.jviromet.2021.114230_bib0120 article-title: Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China publication-title: Lancet doi: 10.1016/S0140-6736(20)30183-5 – volume: 18 start-page: 91 issue: 3 year: 2018 ident: 10.1016/j.jviromet.2021.114230_bib0020 article-title: User’s guide to correlation coefficients publication-title: Turk. J. Emerg. Med. doi: 10.1016/j.tjem.2018.08.001 – volume: 31 start-page: 115 issue: 2 year: 1982 ident: 10.1016/j.jviromet.2021.114230_bib0205 article-title: An extension of shapiro and Wilk’s W test for normality to large samples publication-title: Appl. Stat. doi: 10.2307/2347973 – volume: 4 start-page: 191 year: 2016 ident: 10.1016/j.jviromet.2021.114230_bib0175 article-title: Enabling precision medicine with digital case classification at the point-of-care publication-title: EBioMedicine doi: 10.1016/j.ebiom.2016.01.008 – year: 2020 ident: 10.1016/j.jviromet.2021.114230_bib0195 article-title: Metropolitan wastewater analysis for COVID-19 epidemiological surveillance publication-title: medRxiv – year: 2021 ident: 10.1016/j.jviromet.2021.114230_bib0105 article-title: Implementing building-level SARS-CoV-2 wastewater surveillance on a university campus – volume: 7 start-page: 2409 issue: 1 year: 2017 ident: 10.1016/j.jviromet.2021.114230_bib0230 article-title: Droplet Digital PCR versus qPCR for gene expression analysis with low abundant targets: from variable nonsense to publication quality data publication-title: Sci. Rep. doi: 10.1038/s41598-017-02217-x |
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•Wastewater surveillance methods captured the emergence of SARS-CoV-2 presence in a community.•In a direct comparison of workflows, RT-ddPCR... Throughout the COVID-19 global pandemic there has been significant interest and investment in using Wastewater-Based Epidemiology (WBE) for surveillance of... |
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| SubjectTerms | COVID-19 detection limit Humans Molecular methods monitoring North Carolina nucleic acids nucleocapsid proteins pandemic pathogens PCR Real-Time Polymerase Chain Reaction Reproducibility of Results RNA, Viral - genetics sanitation SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 Virginia Wastewater |
| Title | Assessing sensitivity and reproducibility of RT-ddPCR and RT-qPCR for the quantification of SARS-CoV-2 in wastewater |
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