Evaluating SARS-CoV-2 spike and nucleocapsid proteins as targets for antibody detection in severe and mild COVID-19 cases using a Luminex bead-based assay

•We evaluated a bead-based Luminex assay for the detection of SARS-CoV-2 antibodies.•A combination of the nucleocapsid protein and the receptor-binding domain results in a highly sensitive (96 %) and specific (99 %) assay that can detect positive cases at least five months after infection.•Because w...

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Bibliographic Details
Published in:	Journal of virological methods Vol. 288; p. 114025
Main Authors:	Mariën, Joachim, Ceulemans, Ann, Michiels, Johan, Heyndrickx, Leo, Kerkhof, Karen, Foque, Nikki, Widdowson, Marc-Alain, Mortgat, Laure, Duysburgh, Els, Desombere, Isabelle, Jansens, Hilde, Van Esbroeck, Marjan, Ariën, Kevin K.
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01.02.2021 The Author(s). Published by Elsevier B.V
Subjects:	Antibodies, Neutralizing Antibodies, Viral - immunology antibody detection Bead-based assay cost effectiveness COVID-19 - diagnosis COVID-19 - immunology COVID-19 - virology COVID-19 infection detection limit Humans Immunoassay - methods Immunoassay - standards Immunoglobulin A - blood Immunoglobulin A - immunology Immunoglobulin G - blood Immunoglobulin G - immunology Immunoglobulin M - blood Immunoglobulin M - immunology Luminex antibody test Neutralization Tests nucleocapsid nucleocapsid proteins Nucleocapsid Proteins - immunology ROC Curve Sars-CoV-2 SARS-CoV-2 - immunology Serosurveillance Severe acute respiratory syndrome coronavirus Severe acute respiratory syndrome coronavirus 2 Spike Glycoprotein, Coronavirus - immunology Virus neutralization test Serosurveillance Luminex antibody test Virus neutralization test Sars-CoV-2 Bead-based assay
ISSN:	0166-0934, 1879-0984, 1879-0984
Online Access:	Get full text
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