CRISPR-Cas orthologues and variants: optimizing the repertoire, specificity and delivery of genome engineering tools

Robust and cost-effective genome editing in a diverse array of cells and model organisms is now possible thanks to the discovery of the RNA-guided endonucleases of the CRISPR-Cas system. The commonly used Cas9 of Streptococcus pyogenes shows high levels of activity but, depending on the application,...

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Bibliographic Details
Published in:	Mammalian genome Vol. 28; no. 7-8; pp. 247 - 261
Main Authors:	Cebrian-Serrano, Alberto, Davies, Benjamin
Format:	Journal Article
Language:	English
Published:	New York Springer US 01.08.2017 Springer Nature B.V
Subjects:	Animal Genetics and Genomics Animals Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Biomedical and Life Sciences Cell Biology cost effectiveness CRISPR CRISPR-Associated Protein 9 CRISPR-Cas Systems Deoxyribonucleases, Type II Site-Specific - genetics Deoxyribonucleases, Type II Site-Specific - metabolism DNA-Binding Proteins - chemistry DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Endonucleases - chemistry Endonucleases - genetics Endonucleases - metabolism engineering Enzymes Gene Editing Gene Transfer Techniques Genetic Engineering - methods Genetic Therapy Genome editing Genomes Human Genetics Humans Life Sciences molecular weight Mutagenesis Nuclease nucleases nucleotide sequences Protein Binding Protein Interaction Domains and Motifs proteins Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Ribonucleic acid RNA RNA, Guide, CRISPR-Cas Systems Streptococcus pyogenes Substrate Specificity Target recognition Therapeutic applications virion Wild-type SpCas9 Zinc Finger Nucleases (ZFNs) Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Cas9 Orthologs Sacaan
ISSN:	0938-8990, 1432-1777, 1432-1777
Online Access:	Get full text
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