Inducible, reversible system for the rapid and complete degradation of proteins in mammalian cells

Inducible degradation is a powerful approach for identifying the function of a specific protein or protein complex. Recently, a plant auxin-inducible degron (AID) system has been shown to degrade AID-tagged target proteins in nonplant cells. Here, we demonstrate that an AID-tagged protein can functi...

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Bibliographic Details

Published in:	Proceedings of the National Academy of Sciences - PNAS Vol. 109; no. 49; p. E3350
Main Authors:	Holland, Andrew J, Fachinetti, Daniele, Han, Joo Seok, Cleveland, Don W
Format:	Journal Article
Language:	English
Published:	United States 04.12.2012
Subjects:	Cell Line, Tumor Gene Expression Regulation - genetics Half-Life Humans Indoleacetic Acids - metabolism Kinetics M Phase Cell Cycle Checkpoints - genetics M Phase Cell Cycle Checkpoints - physiology Molecular Biology - methods Proteins - metabolism Proteolysis RNA Interference SKP Cullin F-Box Protein Ligases - metabolism
ISSN:	1091-6490, 1091-6490
Online Access:	Get more information
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