Alevin efficiently estimates accurate gene abundances from dscRNA-seq data

We introduce alevin, a fast end-to-end pipeline to process droplet-based single-cell RNA sequencing data, performing cell barcode detection, read mapping, unique molecular identifier (UMI) deduplication, gene count estimation, and cell barcode whitelisting. Alevin’s approach to UMI deduplication con...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Genome Biology Jg. 20; H. 1; S. 65
Hauptverfasser:	Srivastava, Avi, Malik, Laraib, Smith, Tom, Sudbery, Ian, Patro, Rob
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	London BioMed Central 27.03.2019 Springer Nature B.V BMC
Schlagworte:	Algorithms Animal Genetics and Genomics Animals Bioinformatics Biomedical and Life Sciences Cellular barcode Datasets DNA Barcoding, Taxonomic Evolutionary Biology Gene expression Gene mapping genes Genomes Genomics Human Genetics Humans Life Sciences Method Mice Microbial Genetics and Genomics nucleotide sequences pipelines Plant Genetics and Genomics Quantification Ribonucleic acid RNA sequence analysis Sequence Analysis, RNA Single-Cell Analysis Single-cell RNA-seq Software Transcription UMI deduplication Cellular barcode UMI deduplication Quantification Single-cell RNA-seq
ISSN:	1474-760X, 1474-7596, 1474-760X
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!