Detection of Two Highly Diverse Peribunyaviruses in Mosquitoes from Palenque, Mexico

The Peribunyaviridae family contains the genera Orthobunyavirus, Herbevirus, Pacuvirus, and Shangavirus. Orthobunyaviruses and pacuviruses are mainly transmitted by blood-feeding insects and infect a variety of vertebrates whereas herbeviruses and shangaviruses have a host range restricted to insect...

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Vydáno v:Viruses Ročník 11; číslo 9; s. 832
Hlavní autoři: Kopp, Anne, Hübner, Alexandra, Zirkel, Florian, Hobelsberger, Daniel, Estrada, Alejandro, Jordan, Ingo, Gillespie, Thomas R., Drosten, Christian, Junglen, Sandra
Médium: Journal Article
Jazyk:angličtina
Vydáno: Switzerland MDPI AG 07.09.2019
MDPI
Témata:
arbovirus
Cell culture
chickens
Culicidae
ducks
Endemic species
genome
Genomes
hematophagous insects
Host range
Host specificity
insect-specific virus
Mammalian cells
mammals
Mexico
mosquito
Mosquitoes
National parks
New genera
new genus
Orthobunyavirus
Peribunyaviridae
Phylogenetics
Polymerase chain reaction
Proteins
Rainforests
RNA polymerase
Rooting
Thermal cycling
tropical rain forests
Vertebrates
Viruses
South America
Mexico
United States > US
Germany
Mexico
orthobunyavirus
Peribunyaviridae
insect-specific virus
arbovirus
mosquito
ISSN:1999-4915, 1999-4915
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Shrnutí:The Peribunyaviridae family contains the genera Orthobunyavirus, Herbevirus, Pacuvirus, and Shangavirus. Orthobunyaviruses and pacuviruses are mainly transmitted by blood-feeding insects and infect a variety of vertebrates whereas herbeviruses and shangaviruses have a host range restricted to insects. Here, we tested mosquitoes from a tropical rainforest in Mexico for infections with peribunyaviruses. We identified and characterized two previously unknown viruses, designated Baakal virus (BKAV) and Lakamha virus (LAKV). Sequencing and de novo assembly of the entire BKAV and LAKV genomes revealed that BKAV is an orthobunyavirus and LAKV is likely to belong to a new genus. LAKV was almost equidistant to the established peribunyavirus genera and branched as a deep rooting solitary lineage basal to herbeviruses. Virus isolation attempts of LAKV failed. BKAV is most closely related to the bird-associated orthobunyaviruses Koongol virus and Gamboa virus. BKAV was successfully isolated in mosquito cells but did not replicate in common mammalian cells from various species and organs. Also cells derived from chicken were not susceptible. Interestingly, BKAV can infect cells derived from a duck species that is endemic in the region where the BKAV-positive mosquito was collected. These results suggest a narrow host specificity and maintenance in a mosquito–bird transmission cycle.
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ISSN:1999-4915
1999-4915
DOI:10.3390/v11090832