Possible Arbovirus Found in Virome of Melophagus ovinus

Members of the Lipopteninae subfamily are blood-sucking ectoparasites of mammals. The sheep ked (Melophagus ovinus) is a widely distributed ectoparasite of sheep. It can be found in most sheep-rearing areas and can cause skin irritation, restlessness, anemia, weight loss and skin injuries. Various b...

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Vydáno v:Viruses Ročník 13; číslo 12; s. 2375
Hlavní autoři: Litov, Alexander G., Belova, Oxana A., Kholodilov, Ivan S., Gadzhikurbanov, Magomed N., Gmyl, Larissa V., Oorzhak, Natalia D., Saryglar, Anna A., Ishmukhametov, Aydar A., Karganova, Galina G.
Médium: Journal Article
Jazyk:angličtina
Vydáno: Switzerland MDPI AG 26.11.2021
MDPI
Témata:
anemia
Animals
arboviruses
Arboviruses - genetics
Arboviruses - isolation & purification
Arthropods
Cell Line
cell lines
Dengue fever
Diptera - virology
Ectoparasites
Ectoparasitic Infestations - virology
genome
Genomes
Iflaviridae
Irritation
Kidneys
Mammalian cells
Mammals
Melophagus ovinus
metagenomics
Mitochondrial DNA
Phylogenetics
Phylogeny
Reoviridae
Republic of Tuva
Rhabdoviridae
RNA
Russia
Sheep
Sheep Diseases - parasitology
Siberia
skin irritation
Solemoviridae
swine
Virome
virus
Viruses
weight loss
Russia
Tuva
Lithuania
United States > US
virome
Iflaviridae
Melophagus ovinus
Rhabdoviridae
metagenomics
Solemoviridae
Republic of Tuva
virus
Reoviridae
Sigmavirus
ISSN:1999-4915, 1999-4915
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Shrnutí:Members of the Lipopteninae subfamily are blood-sucking ectoparasites of mammals. The sheep ked (Melophagus ovinus) is a widely distributed ectoparasite of sheep. It can be found in most sheep-rearing areas and can cause skin irritation, restlessness, anemia, weight loss and skin injuries. Various bacteria and some viruses have been detected in M. ovinus; however, the virome of this ked has never been studied using modern approaches. Here, we study the virome of M. ovinus collected in the Republic of Tuva, Russia. In our research, we were able to assemble full genomes for five novel viruses, related to the Rhabdoviridae (Sigmavirus), Iflaviridae, Reoviridae and Solemoviridae families. Four viruses were found in all five of the studied pools, while one virus was found in two pools. Phylogenetically, all of the novel viruses clustered together with various recently described arthropod viruses. All the discovered viruses were tested on their ability to replicate in the mammalian porcine embryo kidney (PEK) cell line. Aksy-Durug Melophagus sigmavirus RNA was detected in the PEK cell line cultural supernate after the first, second and third passages. Such data imply that this virus might be able to replicate in mammalian cells, and thus, can be considered as a possible arbovirus.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1999-4915
1999-4915
DOI:10.3390/v13122375