Line-field confocal optical coherence tomography for high-resolution noninvasive imaging of skin tumors

An optical technique called line-field confocal optical coherence tomography (LC-OCT) is introduced for high-resolution, noninvasive imaging of human skin in vivo. LC-OCT combines the principles of time-domain optical coherence tomography and confocal microscopy with line illumination and detection...

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Veröffentlicht in:Journal of biomedical optics Jg. 23; H. 10; S. 1
Hauptverfasser: Dubois, Arnaud, Levecq, Olivier, Azimani, Hicham, Siret, David, Barut, Anaïs, Suppa, Mariano, Del Marmol, Véronique, Malvehy, Josep, Cinotti, Elisa, Rubegni, Pietro, Perrot, Jean-Luc
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 01.10.2018
Schlagworte:
skin cancer
confocal microscopy
optical coherence tomography
optical imaging
ISSN:1560-2281, 1560-2281
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Zusammenfassung:An optical technique called line-field confocal optical coherence tomography (LC-OCT) is introduced for high-resolution, noninvasive imaging of human skin in vivo. LC-OCT combines the principles of time-domain optical coherence tomography and confocal microscopy with line illumination and detection using a broadband laser and a line-scan camera. LC-OCT measures the echo-time delay and amplitude of light backscattered from cutaneous microstructures through low-coherence interferometry associated with confocal spatial filtering. Multiple A-scans are acquired simultaneously while dynamically adjusting the focus. The resulting cross-sectional B-scan image is produced in real time at 10  frame  /  s. With an isotropic spatial resolution of ∼1  μm, the LC-OCT images reveal a comprehensive structural mapping of skin at the cellular level down to a depth of ∼500  μm. LC-OCT has been applied to the imaging of various skin lesions, in vivo, including carcinomas and melanomas. LC-OCT images are found to strongly correlate with conventional histopathological images. The use of LC-OCT as an adjunct tool in medical practice could significantly improve clinical diagnostic accuracy while reducing the number of biopsies of benign lesions.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:1560-2281
1560-2281
DOI:10.1117/1.JBO.23.10.106007