Comparative Evaluation of Existing and Rationally Designed Novel Antimicrobial Peptides for Treatment of Skin and Soft Tissue Infections

Skin and soft tissue infections (SSTIs) and acne are among the most common skin conditions in primary care. SSTIs caused by ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter sp.) can range in severi...

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Bibliographic Details
Published in:Antibiotics (Basel) Vol. 12; no. 3; p. 551
Main Authors: Ramata-Stunda, Anna, Boroduskis, Martins, Kaktina, Elza, Patetko, Liene, Kalnenieks, Uldis, Lasa, Zane, Rubina, Marta, Strazdina, Inese, Kalnins, Gints, Rutkis, Reinis
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 01.03.2023
MDPI
Subjects:
Abscesses
Acinetobacter baumannii
Acne
Antibacterial activity
Antibacterial agents
Antibiotic resistance
Antibiotics
Antimicrobial agents
Antimicrobial peptides
Bacteria
Bacterial infections
Care and treatment
Comparative analysis
Cutibacterium acnes
Cytokines
Cytotoxicity
Dermatologic agents
Dermatology
Drug resistance
Drug resistance in microorganisms
E coli
Enterobacter
Enterococcus faecium
Escherichia coli
Fibroblasts
Formulae, receipts, prescriptions
Gram-positive bacteria
Health aspects
Infection
Infections
Interleukin 10
Klebsiella
Klebsiella pneumoniae
Leukocytes (mononuclear)
Microorganisms
Minimum inhibitory concentration
Pathogenesis
Pathogens
Peptides
Peripheral blood mononuclear cells
Primary care
Pseudomonas aeruginosa
Scientific equipment and supplies industry
Skin
Skin diseases
skin pathogens
Soft tissues
Staphylococcus aureus
Staphylococcus aureus infections
Toxicity
Tumor necrosis factor-α
United Kingdom
Switzerland
Latvia
acne
skin pathogens
antimicrobial peptides
cytotoxicity
ISSN:2079-6382, 2079-6382
Online Access:Get full text
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Summary:Skin and soft tissue infections (SSTIs) and acne are among the most common skin conditions in primary care. SSTIs caused by ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter sp.) can range in severity, and treating them is becoming increasingly challenging due to the growing number of antibiotic-resistant pathogens. There is also a rise in antibiotic-resistant strains of Cutibacterium acne, which plays a role in the development of acne. Antimicrobial peptides (AMPs) are considered to be a promising solution to the challenges posed by antibiotic resistance. In this study, six new AMPs were rationally designed and compared to five existing peptides. The MIC values against E. coli, P. aeruginosa, K. pneumoniae, E. faecium, S. aureus, and C. acnes were determined, and the peptides were evaluated for cytotoxicity using Balb/c 3T3 cells and dermal fibroblasts, as well as for hemolytic activity. The interaction with bacterial membranes and the effect on TNF-α and IL-10 secretion were also evaluated for selected peptides. Of the tested peptides, RP556 showed high broad-spectrum antibacterial activity without inducing cytotoxicity or hemolysis, and it stimulated the production of IL-10 in LPS-stimulated peripheral blood mononuclear cells. Four of the novel AMPs showed pronounced specificity against C. acnes, with MIC values (0.3–0.5 μg/mL) below the concentrations that were cytotoxic or hemolytic.
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ISSN:2079-6382
2079-6382
DOI:10.3390/antibiotics12030551