Analysis of mitochondrial respiration and ATP synthase in frozen brain tissues

Studying mitochondrial respiration capacity is essential for gaining insights into mitochondrial functions. In frozen tissue samples, however, our ability to study mitochondrial respiration is restricted by damage elicited to the inner mitochondrial membranes by freeze-thaw cycles. We developed an a...

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Bibliographic Details
Published in:Heliyon Vol. 9; no. 3; p. e13888
Main Authors: Yao, Pamela J., Munk, Rachel, Gorospe, Myriam, Kapogiannis, Dimitrios
Format: Journal Article
Language:English
Published: England Elsevier Ltd 01.03.2023
Elsevier
Subjects:
ATP synthase
Brain
Development
Electron transport chain
Frozen samples
Mitochondrial respiration
Electron transport chain
Mitochondrial respiration
Development
Brain
Frozen samples
ATP synthase
ISSN:2405-8440, 2405-8440
Online Access:Get full text
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Summary:Studying mitochondrial respiration capacity is essential for gaining insights into mitochondrial functions. In frozen tissue samples, however, our ability to study mitochondrial respiration is restricted by damage elicited to the inner mitochondrial membranes by freeze-thaw cycles. We developed an approach that combines multiple assays and is tailored towards assessing mitochondrial electron transport chain and ATP synthase in frozen tissues. Using small amounts of frozen tissue, we systematically analyzed the quantity as well as activity of both the electron transport chain complexes and ATP synthase in rat brains during postnatal development. We reveal a previously little-known pattern of increasing mitochondrial respiration capacity with brain development. In addition to providing proof-of-principle evidence that mitochondrial activity changes during brain development, our study details an approach that can be applicable to many other types of frozen cell or tissue samples. •Workflow for measuring mitochondrial respiration in frozen tissues and cells.•Uses small sample amount and commercially available reagents.•Proof-of-principle experiment reveals respiration pattern in developing rat brain.•Applicable to animal and human samples, bridging basic science and clinical studies.
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ISSN:2405-8440
2405-8440
DOI:10.1016/j.heliyon.2023.e13888