Therapeutic Drug Monitoring by Dried Blood Spot: Progress to Date and Future Directions

This article discusses dried blood spot (DBS) sampling in therapeutic drug monitoring (TDM). The most important advantages of DBS sampling in TDM are the minimally invasive procedure of a finger prick (home sampling), the small volume (children), and the stability of the analyte. Many assays in DBS...

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Veröffentlicht in:Clinical pharmacokinetics Jg. 53; H. 11; S. 961 - 973
Hauptverfasser: Wilhelm, Abraham J., den Burger, Jeroen C. G., Swart, Eleonora L.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Cham Springer International Publishing 01.11.2014
Adis International
Springer Nature B.V
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ISSN:0312-5963, 1179-1926, 1179-1926
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Abstract This article discusses dried blood spot (DBS) sampling in therapeutic drug monitoring (TDM). The most important advantages of DBS sampling in TDM are the minimally invasive procedure of a finger prick (home sampling), the small volume (children), and the stability of the analyte. Many assays in DBS have been reported in the literature over the previous 5 years. These assays and their analytical techniques are reviewed here. Factors that may influence the accuracy and reproducibility of DBS methods are also discussed. Important issues are the correlation with plasma/serum concentrations and the influence of hematocrit on spot size and recovery. The different substrate materials are considered. DBS sampling can be a valid alternative to conventional venous sampling. However, patient correlation studies are indispensable to prove this. Promising developments are dried plasma spots using membrane and hematocrit correction using the potassium concentration.
AbstractList This article discusses dried blood spot (DBS) sampling in therapeutic drug monitoring (TDM). The most important advantages of DBS sampling in TDM are the minimally invasive procedure of a finger prick (home sampling), the small volume (children), and the stability of the analyte. Many assays in DBS have been reported in the literature over the previous 5 years. These assays and their analytical techniques are reviewed here. Factors that may influence the accuracy and reproducibility of DBS methods are also discussed. Important issues are the correlation with plasma/serum concentrations and the influence of hematocrit on spot size and recovery. The different substrate materials are considered. DBS sampling can be a valid alternative to conventional venous sampling. However, patient correlation studies are indispensable to prove this. Promising developments are dried plasma spots using membrane and hematocrit correction using the potassium concentration.
This article discusses dried blood spot (DBS) sampling in therapeutic drug monitoring (TDM). The most important advantages of DBS sampling in TDM are the minimally invasive procedure of a finger prick (home sampling), the small volume (children), and the stability of the analyte. Many assays in DBS have been reported in the literature over the previous 5 years. These assays and their analytical techniques are reviewed here. Factors that may influence the accuracy and reproducibility of DBS methods are also discussed. Important issues are the correlation with plasma/serum concentrations and the influence of hematocrit on spot size and recovery. The different substrate materials are considered. DBS sampling can be a valid alternative to conventional venous sampling. However, patient correlation studies are indispensable to prove this. Promising developments are dried plasma spots using membrane and hematocrit correction using the potassium concentration.This article discusses dried blood spot (DBS) sampling in therapeutic drug monitoring (TDM). The most important advantages of DBS sampling in TDM are the minimally invasive procedure of a finger prick (home sampling), the small volume (children), and the stability of the analyte. Many assays in DBS have been reported in the literature over the previous 5 years. These assays and their analytical techniques are reviewed here. Factors that may influence the accuracy and reproducibility of DBS methods are also discussed. Important issues are the correlation with plasma/serum concentrations and the influence of hematocrit on spot size and recovery. The different substrate materials are considered. DBS sampling can be a valid alternative to conventional venous sampling. However, patient correlation studies are indispensable to prove this. Promising developments are dried plasma spots using membrane and hematocrit correction using the potassium concentration.
Author Swart, Eleonora L.
den Burger, Jeroen C. G.
Wilhelm, Abraham J.
Author_xml – sequence: 1
  givenname: Abraham J.
  surname: Wilhelm
  fullname: Wilhelm, Abraham J.
  email: aj.wilhelm@vumc.nl
  organization: Department of Clinical Pharmacology and Pharmacy, VU University Medical Center
– sequence: 2
  givenname: Jeroen C. G.
  surname: den Burger
  fullname: den Burger, Jeroen C. G.
  organization: Department of Clinical Pharmacology and Pharmacy, VU University Medical Center
– sequence: 3
  givenname: Eleonora L.
  surname: Swart
  fullname: Swart, Eleonora L.
  organization: Department of Clinical Pharmacology and Pharmacy, VU University Medical Center
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Issue 11
Keywords Paper Spray
Guanfacine
Finger Prick
Therapeutic Drug Monitoring
Proficiency Testing Program
Human
Therapeutic drug monitoring
Biological fluid
Updating
Blood
Language English
License CC BY 4.0
Open AccessThis article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
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Snippet This article discusses dried blood spot (DBS) sampling in therapeutic drug monitoring (TDM). The most important advantages of DBS sampling in TDM are the...
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StartPage 961
SubjectTerms Biological and medical sciences
Blood Specimen Collection
Dried Blood Spot Testing - methods
Dried Blood Spot Testing - trends
Drug Monitoring - methods
Drug Monitoring - trends
General pharmacology
Hematocrit
Humans
Immunosuppressive Agents - blood
Internal Medicine
Medical sciences
Medicine
Medicine & Public Health
Netherlands
Pharmacokinetics. Pharmacogenetics. Drug-receptor interactions
Pharmacology. Drug treatments
Pharmacology/Toxicology
Pharmacotherapy
Plasma - chemistry
Reproducibility of Results
Review
Review Article
Tandem Mass Spectrometry
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Title Therapeutic Drug Monitoring by Dried Blood Spot: Progress to Date and Future Directions
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