Disaccharide compositional analysis of heparan sulfate and heparin polysaccharides using UV or high-sensitivity fluorescence (BODIPY) detection

One of the first steps in characterizing heparan sulfate (HS) and its close relative heparin is to conduct disaccharide composition analysis. This provides an overall picture of the structure of the polysaccharide in terms of its constituent disaccharides. This is of importance, for example, in the...

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Bibliographic Details
Published in:	Nature protocols Vol. 5; no. 12; pp. 1983 - 1992
Main Authors:	Skidmore, Mark A, Guimond, Scott E, Dumax-Vorzet, Audrey F, Yates, Edwin A, Turnbull, Jeremy E
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 01.12.2010 Nature Publishing Group
Subjects:	631/1647/2230/1378 631/1647/666/2260 631/45/72/1205 Analytical Chemistry Anticoagulants Biological Techniques Biomedical and Life Sciences Boron Compounds Carbohydrates Chromatography, High Pressure Liquid - methods Composition Computational Biology/Bioinformatics Disaccharides - isolation & purification Enzymes Fluorescence Fluorescent Dyes Heparan sulfate Heparin Heparin - analysis Heparitin Sulfate - analysis High performance liquid chromatography Life Sciences Liquid chromatography Methods Microarrays Organic Chemistry Properties protocol Saccharides Spectrophotometry, Ultraviolet - methods Sugars Sulfates United Kingdom United Kingdom > UK
ISSN:	1754-2189, 1750-2799, 1750-2799
Online Access:	Get full text
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