High-Speed MALDI-TOF Imaging Mass Spectrometry: Rapid Ion Image Acquisition and Considerations for Next Generation Instrumentation

A prototype matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometer has been used for high-speed ion image acquisition. The instrument incorporates a Nd:YLF solid state laser capable of pulse repetition rates up to 5 kHz and continuous laser raster sampling for high-...

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Bibliographic Details
Published in:Journal of the American Society for Mass Spectrometry Vol. 22; no. 6; pp. 1022 - 1031
Main Authors: Spraggins, Jeffrey M., Caprioli, Richard M.
Format: Journal Article
Language:English
Published: New York Springer-Verlag 01.06.2011
Elsevier
Springer Nature B.V
Subjects:
Analytical Chemistry
Angiotensin II - chemistry
Animals
Bioinformatics
Biological and medical sciences
Biotechnology
Brain
Brain Chemistry
Chemistry
Chemistry and Materials Science
Data acquisition
Desorption
Focus: MALDI-TOF and Biological MS: Research Article
High speed
Image acquisition
Image Processing, Computer-Assisted - methods
Imaging
Instruments
Investigative techniques, diagnostic techniques (general aspects)
Ionization
Ions
Lasers
Lipids - chemistry
Mass spectrometry
Medical sciences
Miscellaneous. Technology
New technology
Organic Chemistry
Oversampling
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Proteomics
Raster
Rats
Repetition
Sampling
Scientific imaging
Signal Processing, Computer-Assisted
Solid state lasers
Spatial resolution
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Spectroscopy
Microprobe
MALDI
Imaging
Lipids
TOF
Mass spectrometry
Continuous laser raster
Rat
Signal sampling
Rodentia
Central nervous system
Raster
Imaging mass spectrometry
Instrumentation
Matrix assisted laser desorption ionization
Encephalon
Vertebrata
Data acquisition system
Mammalia
Time of flight method
Animal
CW laser
ISSN:1044-0305, 1879-1123, 1879-1123
Online Access:Get full text
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Description
Summary:A prototype matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometer has been used for high-speed ion image acquisition. The instrument incorporates a Nd:YLF solid state laser capable of pulse repetition rates up to 5 kHz and continuous laser raster sampling for high-throughput data collection. Lipid ion images of a sagittal rat brain tissue section were collected in 10 min with an effective acquisition rate of roughly 30 pixels/s. These results represent more than a 10-fold increase in throughput compared with current commercially available instrumentation. Experiments aimed at improving conditions for continuous laser raster sampling for imaging are reported, highlighting proper laser repetition rates and stage velocities to avoid signal degradation from significant oversampling. As new high spatial resolution and large sample area applications present themselves, the development of high-speed microprobe MALDI imaging mass spectrometry is essential to meet the needs of those seeking new technologies for rapid molecular imaging.
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ISSN:1044-0305
1879-1123
1879-1123
DOI:10.1007/s13361-011-0121-0