High-Speed MALDI-TOF Imaging Mass Spectrometry: Rapid Ion Image Acquisition and Considerations for Next Generation Instrumentation

A prototype matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometer has been used for high-speed ion image acquisition. The instrument incorporates a Nd:YLF solid state laser capable of pulse repetition rates up to 5 kHz and continuous laser raster sampling for high-...

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Vydané v:	Journal of the American Society for Mass Spectrometry Ročník 22; číslo 6; s. 1022 - 1031
Hlavní autori:	Spraggins, Jeffrey M., Caprioli, Richard M.
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	New York Springer-Verlag 01.06.2011 Elsevier Springer Nature B.V
Predmet:	Analytical Chemistry Angiotensin II - chemistry Animals Bioinformatics Biological and medical sciences Biotechnology Brain Brain Chemistry Chemistry Chemistry and Materials Science Data acquisition Desorption Focus: MALDI-TOF and Biological MS: Research Article High speed Image acquisition Image Processing, Computer-Assisted - methods Imaging Instruments Investigative techniques, diagnostic techniques (general aspects) Ionization Ions Lasers Lipids - chemistry Mass spectrometry Medical sciences Miscellaneous. Technology New technology Organic Chemistry Oversampling Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Proteomics Raster Rats Repetition Sampling Scientific imaging Signal Processing, Computer-Assisted Solid state lasers Spatial resolution Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Spectroscopy Microprobe MALDI Imaging Lipids TOF Mass spectrometry Continuous laser raster Rat Signal sampling Rodentia Central nervous system Raster Imaging mass spectrometry Instrumentation Matrix assisted laser desorption ionization Encephalon Vertebrata Data acquisition system Mammalia Time of flight method Animal CW laser
ISSN:	1044-0305, 1879-1123, 1879-1123
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Shrnutí:	A prototype matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometer has been used for high-speed ion image acquisition. The instrument incorporates a Nd:YLF solid state laser capable of pulse repetition rates up to 5 kHz and continuous laser raster sampling for high-throughput data collection. Lipid ion images of a sagittal rat brain tissue section were collected in 10 min with an effective acquisition rate of roughly 30 pixels/s. These results represent more than a 10-fold increase in throughput compared with current commercially available instrumentation. Experiments aimed at improving conditions for continuous laser raster sampling for imaging are reported, highlighting proper laser repetition rates and stage velocities to avoid signal degradation from significant oversampling. As new high spatial resolution and large sample area applications present themselves, the development of high-speed microprobe MALDI imaging mass spectrometry is essential to meet the needs of those seeking new technologies for rapid molecular imaging.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	1044-0305 1879-1123 1879-1123
DOI:	10.1007/s13361-011-0121-0