Validation of Muscle Fiber Architecture of the Human Tongue Revealed by Diffusion Magnetic Resonance Imaging With Histology Verification
The goal of this study is to validate the muscle architecture derived from both ex vivo and in vivo diffusion-weighted magnetic resonance imaging (dMRI) of the human tongue with histology of an ex vivo tongue. dMRI was acquired with a 200-direction high angular resolution diffusion imaging (HARDI) d...
Saved in:
| Published in: | Journal of speech, language, and hearing research Vol. 65; no. 10; p. 3661 |
|---|---|
| Main Authors: | , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
United States
01.10.2022
|
| Subjects: | |
| ISSN: | 1558-9102, 1558-9102 |
| Online Access: | Get more information |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Abstract | The goal of this study is to validate the muscle architecture derived from both ex vivo and in vivo diffusion-weighted magnetic resonance imaging (dMRI) of the human tongue with histology of an ex vivo tongue.
dMRI was acquired with a 200-direction high angular resolution diffusion imaging (HARDI) diffusion scheme for both a postmortem head (imaged within 48 hr after death) and a healthy volunteer. After MRI, the postmortem head was fixed and the tongue excised for hematoxylin and eosin (H&E) staining and histology imaging. Structure tensor images were generated from the stained images to better demonstrate muscle fiber orientations. The tongue muscle fiber orientations, estimated from dMRI, were visualized using the tractogram, a novel representation of crossing fiber orientations, and compared against the histology images of the ex vivo tongue.
Muscle fibers identified in the tractograms showed good correspondence with those appearing in the histology images. We further demonstrated tongue muscle architecture in in vivo tractograms for the entire tongue.
The study demonstrates that dMRI can accurately reveal the complex muscle architecture of the human tongue and may potentially benefit planning and evaluation of oral surgery and research on speech and swallowing. |
|---|---|
| AbstractList | The goal of this study is to validate the muscle architecture derived from both ex vivo and in vivo diffusion-weighted magnetic resonance imaging (dMRI) of the human tongue with histology of an ex vivo tongue.
dMRI was acquired with a 200-direction high angular resolution diffusion imaging (HARDI) diffusion scheme for both a postmortem head (imaged within 48 hr after death) and a healthy volunteer. After MRI, the postmortem head was fixed and the tongue excised for hematoxylin and eosin (H&E) staining and histology imaging. Structure tensor images were generated from the stained images to better demonstrate muscle fiber orientations. The tongue muscle fiber orientations, estimated from dMRI, were visualized using the tractogram, a novel representation of crossing fiber orientations, and compared against the histology images of the ex vivo tongue.
Muscle fibers identified in the tractograms showed good correspondence with those appearing in the histology images. We further demonstrated tongue muscle architecture in in vivo tractograms for the entire tongue.
The study demonstrates that dMRI can accurately reveal the complex muscle architecture of the human tongue and may potentially benefit planning and evaluation of oral surgery and research on speech and swallowing. The goal of this study is to validate the muscle architecture derived from both ex vivo and in vivo diffusion-weighted magnetic resonance imaging (dMRI) of the human tongue with histology of an ex vivo tongue.PURPOSEThe goal of this study is to validate the muscle architecture derived from both ex vivo and in vivo diffusion-weighted magnetic resonance imaging (dMRI) of the human tongue with histology of an ex vivo tongue.dMRI was acquired with a 200-direction high angular resolution diffusion imaging (HARDI) diffusion scheme for both a postmortem head (imaged within 48 hr after death) and a healthy volunteer. After MRI, the postmortem head was fixed and the tongue excised for hematoxylin and eosin (H&E) staining and histology imaging. Structure tensor images were generated from the stained images to better demonstrate muscle fiber orientations. The tongue muscle fiber orientations, estimated from dMRI, were visualized using the tractogram, a novel representation of crossing fiber orientations, and compared against the histology images of the ex vivo tongue.METHODdMRI was acquired with a 200-direction high angular resolution diffusion imaging (HARDI) diffusion scheme for both a postmortem head (imaged within 48 hr after death) and a healthy volunteer. After MRI, the postmortem head was fixed and the tongue excised for hematoxylin and eosin (H&E) staining and histology imaging. Structure tensor images were generated from the stained images to better demonstrate muscle fiber orientations. The tongue muscle fiber orientations, estimated from dMRI, were visualized using the tractogram, a novel representation of crossing fiber orientations, and compared against the histology images of the ex vivo tongue.Muscle fibers identified in the tractograms showed good correspondence with those appearing in the histology images. We further demonstrated tongue muscle architecture in in vivo tractograms for the entire tongue.RESULTSMuscle fibers identified in the tractograms showed good correspondence with those appearing in the histology images. We further demonstrated tongue muscle architecture in in vivo tractograms for the entire tongue.The study demonstrates that dMRI can accurately reveal the complex muscle architecture of the human tongue and may potentially benefit planning and evaluation of oral surgery and research on speech and swallowing.CONCLUSIONThe study demonstrates that dMRI can accurately reveal the complex muscle architecture of the human tongue and may potentially benefit planning and evaluation of oral surgery and research on speech and swallowing. |
| Author | Prince, Jerry L Gullapalli, Rao P Stone, Maureen Zhuo, Jiachen Roys, Steve Liang, Xiao Jiang, Li Elsaid, Nahla M H Puche, Adam C |
| Author_xml | – sequence: 1 givenname: Xiao surname: Liang fullname: Liang, Xiao organization: Department of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, Baltimore – sequence: 2 givenname: Nahla M H surname: Elsaid fullname: Elsaid, Nahla M H organization: Department of Radiology and Biomedical Imaging, Yale School of Medicine, New Haven, CT – sequence: 3 givenname: Li surname: Jiang fullname: Jiang, Li organization: Department of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, Baltimore – sequence: 4 givenname: Steve surname: Roys fullname: Roys, Steve organization: Department of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, Baltimore – sequence: 5 givenname: Adam C surname: Puche fullname: Puche, Adam C organization: Department of Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore – sequence: 6 givenname: Rao P surname: Gullapalli fullname: Gullapalli, Rao P organization: Department of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, Baltimore – sequence: 7 givenname: Maureen surname: Stone fullname: Stone, Maureen organization: Department of Neural and Pain Sciences and Department of Orthodontics, University of Maryland School of Dentistry, Baltimore – sequence: 8 givenname: Jerry L surname: Prince fullname: Prince, Jerry L organization: Department of Electrical and Computer Engineering, Johns Hopkins University, Baltimore, MD – sequence: 9 givenname: Jiachen orcidid: 0000-0001-6637-701X surname: Zhuo fullname: Zhuo, Jiachen organization: Department of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, Baltimore |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/36054846$$D View this record in MEDLINE/PubMed |
| BookMark | eNpNkF9LwzAUxYMozk0_gSB59KWaJmnaPg51brIhzKmPJUlvu0ibaJMK-wZ-bOs_8OkeOL9zLpwx2rfOAkKnMbmICeeXlFBa3D0s5-uI0ogQwskeOoqTJIvymND9f3qExt6_DAiJuThEIyZIwjMujtDHk2xMKYNxFrsKr3qvG8Azo6DD005vTQAd-g6-zLAFPO9bafHG2boHvIZ3kA2UWO3wtamq3n_VrGRtIRg92N5ZaTXgRStrY2v8bMIWz40PrnH1Dj9BZyqjv78fo4NKNh5Ofu8EPc5uNlfzaHl_u7iaLiOdCBaiHGKmmU5FmeSKKkWyijOoSgoqBpGB4DHNc5mVIEqmRKJTlmZCKSa5ljrndILOf3pfO_fWgw9Fa7yGppEWXO8LmpJ8yHDGBvTsF-1VC2Xx2plWdrvibz36CWdIdnI |
| CitedBy_id | crossref_primary_10_1044_2024_JSLHR_23_00125 crossref_primary_10_1016_j_actbio_2023_10_027 |
| ContentType | Journal Article |
| DBID | CGR CUY CVF ECM EIF NPM 7X8 |
| DOI | 10.1044/2022_JSLHR-22-00040 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | no_fulltext_linktorsrc |
| Discipline | Medicine Languages & Literatures Social Welfare & Social Work |
| EISSN | 1558-9102 |
| ExternalDocumentID | 36054846 |
| Genre | Journal Article Research Support, N.I.H., Extramural |
| GrantInformation_xml | – fundername: NIDCD NIH HHS grantid: R01 DC014717 |
| GroupedDBID | --- --Z -W8 -~X .GJ .GO 0-V 04C 0R~ 18M 29L 36B 4.4 5GY 6NX 6PF 7RV 7X7 85S 8A4 8G5 8R4 8R5 AAHSB AAWTL ABDBF ABIVO ABOPQ ABPPZ ABWJO ABZEH ACGFO ACGOD ACHQT ACNCT ACUHS ACUXI ADBBV ADOJX AENEX AERSA AFKRA AGHSJ AHMBA AIKWM ALMA_UNASSIGNED_HOLDINGS ALSLI ARALO AZQEC BENPR BKEYQ BMSDO BPHCQ BVXVI CGR CJNVE CPGLG CRLPW CS3 CUY CVF DU5 EAD EAP EAS EBD EBO EBS ECE ECF ECM ECT EDJ EIF EIHBH EMB EMK EMOBN ESX EX3 F5P F9R FJW FYUFA GUQSH H13 HCIFZ HZ~ I-F IAO ICO IEA IER IHR IHW IN- INH INIJC INR IOF IPO IPY ITC M0P M1P M2M M2O M2P M2Q M2R MLAFT NPM O9- P2P PADUT PCD PQQKQ PROAC PSQYO PSYQQ Q2X QF4 QM7 QN7 QO5 RWL S0X SJA SV3 TAE TH9 TN5 TUS TWZ UHB UKHRP UPT WH7 WOW WQ9 YQT ZCA 7X8 ABUFD |
| ID | FETCH-LOGICAL-c563t-9e13c3c76d59b2bb08f43efd2eb1e68e641299a8de6d3b65c73786bb3a4cac942 |
| IEDL.DBID | 7X8 |
| ISICitedReferencesCount | 3 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000877789100005&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 1558-9102 |
| IngestDate | Sat Nov 01 14:48:46 EDT 2025 Thu Aug 28 04:23:35 EDT 2025 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 10 |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c563t-9e13c3c76d59b2bb08f43efd2eb1e68e641299a8de6d3b65c73786bb3a4cac942 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ORCID | 0000-0001-6637-701X |
| OpenAccessLink | https://www.ncbi.nlm.nih.gov/pmc/articles/9927595 |
| PMID | 36054846 |
| PQID | 2709737433 |
| PQPubID | 23479 |
| ParticipantIDs | proquest_miscellaneous_2709737433 pubmed_primary_36054846 |
| PublicationCentury | 2000 |
| PublicationDate | 2022-10-01 |
| PublicationDateYYYYMMDD | 2022-10-01 |
| PublicationDate_xml | – month: 10 year: 2022 text: 2022-10-01 day: 01 |
| PublicationDecade | 2020 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States |
| PublicationTitle | Journal of speech, language, and hearing research |
| PublicationTitleAlternate | J Speech Lang Hear Res |
| PublicationYear | 2022 |
| SSID | ssj0000146 |
| Score | 2.3932316 |
| Snippet | The goal of this study is to validate the muscle architecture derived from both ex vivo and in vivo diffusion-weighted magnetic resonance imaging (dMRI) of the... |
| SourceID | proquest pubmed |
| SourceType | Aggregation Database Index Database |
| StartPage | 3661 |
| SubjectTerms | Brain Diffusion Magnetic Resonance Imaging - methods Eosine Yellowish-(YS) - analysis Hematoxylin - analysis Humans Magnetic Resonance Imaging - methods Muscle Fibers, Skeletal Tongue - diagnostic imaging |
| Title | Validation of Muscle Fiber Architecture of the Human Tongue Revealed by Diffusion Magnetic Resonance Imaging With Histology Verification |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/36054846 https://www.proquest.com/docview/2709737433 |
| Volume | 65 |
| WOSCitedRecordID | wos000877789100005&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lb9QwELaAIsSFxwKlUNAgIW4Ru7HjOCdUQVcF7a6qUtq9RX6M6aqQlM1uJf4BP5uZJEt7QULikkOeVjy2v5lv_I0Qr1Md5MgH5tTTLFEj7xOLyCUBbfR6GFW0rbr-JJ_NzHxeHPYBt6ZPq9zMie1EHWrPMfK3ac7CMrTeyXcXPxKuGsXsal9C46bYkgRlOKUrn5tr8lHd7qIs40HNmTyd6pBS7POn5afPk4OjhLwxRjbDv2PMdq0Z3__fVj4Q93qUCXudWTwUN7AaiO1JH5ts4A1M_sgpNwNxZ9oz7AOx2-3XhVP8Fu0S6c7NiXp5_kj8OiHc3pVhgjrCdN3Q-2HMaSewd42T4IsELaHlCOC4pg8jHOElw9IA7id8WMS45kgdTO3XindSAjMJLP-B8PF7WzsJTherM2iFTDj2Dyc0WmIfY3wsvoz3j98fJH0xh8RnWq6SAkfSS5_rkBUudW5oopIYQ0qLBWqDWhHyKKwJSNbjdObpNxrtnLTKW1-o9Im4VdUVPhVgcgxeFTJY5ci_C0Zjng8dIRuTpTbaHfFq0zklDRZmQGyF9bopr7pnR2x3PVxedKoepSTHThEae_YPTz8Xd9mAuqS-XbEVaarAF-K2v1wtmuXL1grpODuc_gb4veii |
| linkProvider | ProQuest |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Validation+of+Muscle+Fiber+Architecture+of+the+Human+Tongue+Revealed+by+Diffusion+Magnetic+Resonance+Imaging+With+Histology+Verification&rft.jtitle=Journal+of+speech%2C+language%2C+and+hearing+research&rft.au=Liang%2C+Xiao&rft.au=Elsaid%2C+Nahla+M+H&rft.au=Jiang%2C+Li&rft.au=Roys%2C+Steve&rft.date=2022-10-01&rft.issn=1558-9102&rft.eissn=1558-9102&rft.volume=65&rft.issue=10&rft.spage=3661&rft_id=info:doi/10.1044%2F2022_JSLHR-22-00040&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1558-9102&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1558-9102&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1558-9102&client=summon |