Structural Basis for the Canonical and Non-canonical PAM Recognition by CRISPR-Cpf1

The RNA-guided Cpf1 (also known as Cas12a) nuclease associates with a CRISPR RNA (crRNA) and cleaves the double-stranded DNA target complementary to the crRNA guide. The two Cpf1 orthologs from Acidaminococcus sp. (AsCpf1) and Lachnospiraceae bacterium (LbCpf1) have been harnessed for eukaryotic gen...

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Vydáno v:	Molecular cell Ročník 67; číslo 4; s. 633
Hlavní autoři:	Yamano, Takashi, Zetsche, Bernd, Ishitani, Ryuichiro, Zhang, Feng, Nishimasu, Hiroshi, Nureki, Osamu
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States 17.08.2017
Témata:	Acidaminococcus - enzymology Acidaminococcus - genetics Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Binding Sites Clostridiales - enzymology Clostridiales - genetics Clustered Regularly Interspaced Short Palindromic Repeats CRISPR-Associated Proteins - chemistry CRISPR-Associated Proteins - genetics CRISPR-Associated Proteins - metabolism CRISPR-Cas Systems Crystallography, X-Ray DNA - chemistry DNA - genetics DNA - metabolism Endonucleases - chemistry Endonucleases - genetics Endonucleases - metabolism Escherichia coli - enzymology Escherichia coli - genetics HEK293 Cells Humans Models, Molecular Nucleic Acid Conformation Nucleic Acid Heteroduplexes - chemistry Nucleic Acid Heteroduplexes - genetics Nucleic Acid Heteroduplexes - metabolism Protein Binding Protein Conformation RNA, Guide, CRISPR-Cas Systems - chemistry RNA, Guide, CRISPR-Cas Systems - genetics RNA, Guide, CRISPR-Cas Systems - metabolism Structure-Activity Relationship CRISPR-Cas Cpf1 crystal structure protospacer adjacent motif Cas12a
ISSN:	1097-4164, 1097-4164
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