Rapid lateral-flow immunoassay for the quantum dot-based detection of puerarin

In this study, a rapid (within 10min) quantitative lateral-flow immunoassay using a quantum dots (QDs)-antibody probe was developed for the analysis of puerarin (PUE) in water and biological samples. The competitive immunoassay was based on anti-PUE monoclonal antibody conjugated with QDs (detection...

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Veröffentlicht in:Biosensors & bioelectronics Jg. 81; S. 358 - 362
Hauptverfasser: Qu, Huihua, Zhang, Yue, Qu, Baoping, Kong, Hui, Qin, Gaofeng, Liu, Shuchen, Cheng, Jinjun, Wang, Qingguo, Zhao, Yan
Format: Journal Article
Sprache:Englisch
Veröffentlicht: England Elsevier B.V 15.07.2016
Schlagworte:
Animals
Antibodies, Immobilized - chemistry
Antibodies, Monoclonal - chemistry
Biological
Biosensing Techniques - economics
Biosensing Techniques - instrumentation
Biosensors
Cattle
detection limit
equations
Equipment Design
Fabaceae - chemistry
immunoaffinity chromatography
Immunoassay
immunoassays
Immunochromatography - economics
Immunochromatography - instrumentation
inhibitory concentration 50
Isoflavones - analysis
Lateral flow immunoassay
Mathematical analysis
Membranes
monoclonal antibodies
Monoclonal antibody
Plant Extracts - chemistry
Puerarin
Quantum dot
Quantum dots
Quantum Dots - chemistry
Readers
Regression
screening
serum albumin
Serum Albumin, Bovine - chemistry
Quantum dot
Monoclonal antibody
Puerarin
Lateral flow immunoassay
ISSN:0956-5663, 1873-4235, 1873-4235
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Zusammenfassung:In this study, a rapid (within 10min) quantitative lateral-flow immunoassay using a quantum dots (QDs)-antibody probe was developed for the analysis of puerarin (PUE) in water and biological samples. The competitive immunoassay was based on anti-PUE monoclonal antibody conjugated with QDs (detection reagent). Secondary antibody was immobilized on one end of a nitrocellulose membrane (control line) and PUE-bovine serum albumin conjugate was immobilized on the other end (test line). In the quantitative experiment, the detection results were scanned using a membrane strip reader and a detection curve (regression equation: y=−0.11ln(x)+0.979, R2=0.9816) representing the averages of the scanned data was obtained. This curve was linear from 1 to 10μg/mL. The IC50 value was 75.58ng/mL and the qualitative detection limit of PUE was 5.8ng/mL. The recovery of PUE added to phosphate-buffered saline and biological samples was in the range of 97.38–116.56%. To our knowledge, this is the first report of the quantitative detection of a natural product by QDs-based immunochromatography, which represents a powerful tool for rapidly screening PUE in plant materials and other biological samples. •A lateral-flow immunoassay based on anti-puerarin monoclonal antibody conjugated with quantum dots (QDs) was prepared.•For the bright fluorescent emission of the QDs, puerarin in plant materials and biological samples can be detected more sensitively.•The quantitative immunoassay is rapid (within 10 min), and requires no expensive instrumentation or specialist training.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0956-5663
1873-4235
1873-4235
DOI:10.1016/j.bios.2016.03.008