A recombinant vesicular stomatitis virus replicon vaccine protects chickens from highly pathogenic avian influenza virus (H7N1)

Highly pathogenic avian influenza viruses (HPAIV) of subtypes H5 and H7 cause fatal disease in poultry (fowl plague) but also have zoonotic potential. Currently commercially available vaccines often do not provide sufficient protection and do not allow easy discrimination between vaccinated and infe...

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Vydáno v:Vaccine Ročník 27; číslo 8; s. 1174 - 1183
Hlavní autoři: Kalhoro, Nazeer H., Veits, Jutta, Rautenschlein, Silke, Zimmer, Gert
Médium: Journal Article
Jazyk:angličtina
Vydáno: Kidlington Elsevier Ltd 18.02.2009
Elsevier
Elsevier Limited
Témata:
Allergy and Immunology
Animals
Antibodies, Viral - blood
Antigens
Applied microbiology
Avian influenza virus
Biological and medical sciences
Biosafety
Bird migration
Cell Line
Chick Embryo
Chickens
Cricetinae
Fundamental and applied biological sciences. Psychology
Genetic Vectors
Immunization
Immunization, Secondary
Influenza A virus - immunology
Influenza A Virus, H5N2 Subtype - immunology
Influenza A Virus, H7N7 Subtype - genetics
Influenza A Virus, H7N7 Subtype - immunology
Influenza in Birds - immunology
Influenza in Birds - prevention & control
Influenza Vaccines - administration & dosage
Influenza Vaccines - immunology
Injections, Intramuscular
Microbiology
Miscellaneous
Mortality
Mutation
Neutralization Tests
Poultry
RNA replicon
Vaccines
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Vaccines, Marker - administration & dosage
Vaccines, Marker - immunology
Vesicular stomatitis virus
Vesiculovirus - genetics
Veterinary medicine
Virology
Virus Shedding - immunology
Viruses
Zoonoses
Avian influenza virus
Vesicular stomatitis virus
RNA replicon
Farming animal
Poultry
Recombinant virus
Orthomyxoviridae
Zoopathogen
Vaccine
Virus
Pathogenicity
Mononegavirales
Vertebrata
Vesiculovirus
Influenzavirus A
Avian influenzavirus
Rhabdoviridae
Chicken
Aves
Veterinary
ISSN:0264-410X, 1873-2518
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Shrnutí:Highly pathogenic avian influenza viruses (HPAIV) of subtypes H5 and H7 cause fatal disease in poultry (fowl plague) but also have zoonotic potential. Currently commercially available vaccines often do not provide sufficient protection and do not allow easy discrimination between vaccinated and infected birds. Therefore, vaccination of domestic poultry against H5 and H7 HPAIV is not allowed in many countries, or is only possible after special permission has been provided. We generated a recombinant marker vaccine based on non-transmissible vesicular stomatitis virus (VSV) expressing the HA antigen of HPAIV A/FPV/Rostock/34 (H7N1) in place of the VSV G gene. This virus, VSV*ΔG(HA), was propagated on a helper cell line providing VSV G in trans. Since no progeny virus was produced after infection of non-complementing cells, the vector was classified as biosafety level 1 organism (“safe”). Chickens were immunized via the intramuscular route. Following booster vaccination with the same replicons high titers of serum antibodies were induced, which neutralized avian influenza viruses of subtypes H7N1 and H7N7 but not H5N2. Vaccinated chickens were protected against a lethal dose of heterologous HPAIV A/chicken/Italy/445/99 (H7N1). Secretion of challenge virus was short-term and significantly reduced. Finally, it was possible to discriminate vaccinated chickens from infected ones by a simple ELISA assay. We propose that VSV replicons have the potential to be developed to high-quality vaccines for protection of poultry against different subtypes of avian influenza viruses.
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ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2008.12.019