Creation of a bacterial cell controlled by a chemically synthesized genome

We report the design, synthesis, and assembly of the 1.08-mega-base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new M. mycoides cells that are controlled only by the synthetic ch...

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Vydané v:	Science (American Association for the Advancement of Science) Ročník 329; číslo 5987; s. 52
Hlavní autori:	Gibson, Daniel G, Glass, John I, Lartigue, Carole, Noskov, Vladimir N, Chuang, Ray-Yuan, Algire, Mikkel A, Benders, Gwynedd A, Montague, Michael G, Ma, Li, Moodie, Monzia M, Merryman, Chuck, Vashee, Sanjay, Krishnakumar, Radha, Assad-Garcia, Nacyra, Andrews-Pfannkoch, Cynthia, Denisova, Evgeniya A, Young, Lei, Qi, Zhi-Qing, Segall-Shapiro, Thomas H, Calvey, Christopher H, Parmar, Prashanth P, Hutchison, 3rd, Clyde A, Smith, Hamilton O, Venter, J Craig
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States 02.07.2010
Predmet:	Bacterial Proteins - analysis Base Sequence Bioengineering Cloning, Molecular DNA, Bacterial - chemical synthesis DNA, Bacterial - genetics Escherichia coli - genetics Gene Deletion Genes, Bacterial Genetic Engineering Genome, Bacterial Molecular Sequence Data Mycoplasma capricolum - genetics Mycoplasma mycoides - genetics Mycoplasma mycoides - growth & development Mycoplasma mycoides - physiology Mycoplasma mycoides - ultrastructure Phenotype Plasmids Polymerase Chain Reaction Polymorphism, Genetic Saccharomyces cerevisiae - genetics Transformation, Bacterial
ISSN:	1095-9203, 1095-9203
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Shrnutí:	We report the design, synthesis, and assembly of the 1.08-mega-base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new M. mycoides cells that are controlled only by the synthetic chromosome. The only DNA in the cells is the designed synthetic DNA sequence, including "watermark" sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process. The new cells have expected phenotypic properties and are capable of continuous self-replication.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1095-9203 1095-9203
DOI:	10.1126/science.1190719