Deep Learning‐Assisted Automated Single Cell Electroporation Platform for Effective Genetic Manipulation of Hard‐to‐Transfect Cells

Genome engineering of cells using CRISPR/Cas systems has opened new avenues for pharmacological screening and investigating the molecular mechanisms of disease. A critical step in many such studies is the intracellular delivery of the gene editing machinery and the subsequent manipulation of cells....

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Bibliographic Details
Published in:	Small (Weinheim an der Bergstrasse, Germany) Vol. 18; no. 20; pp. e2107795 - n/a
Main Authors:	Mukherjee, Prithvijit, Patino, Cesar A., Pathak, Nibir, Lemaitre, Vincent, Espinosa, Horacio D.
Format:	Journal Article
Language:	English
Published:	Germany Wiley Subscription Services, Inc 01.05.2022 Wiley Blackwell (John Wiley & Sons)
Subjects:	Algorithms Automation CRISPR CRISPR-Cas Systems - genetics CRISPR/Cas9 Deep Learning Electroporation Electroporation - methods Gene Editing - methods Genetic modification Humans human‐induced pluripotent stem cells (hiPSCs) Induced Pluripotent Stem Cells - metabolism intracellular delivery Machine learning Nanotechnology Screening single‐cell electroporation Stem cells Workflow single-cell electroporation deep learning human-induced pluripotent stem cells (hiPSCs) CRISPR/Cas9 intracellular delivery
ISSN:	1613-6810, 1613-6829, 1613-6829
Online Access:	Get full text
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