Molecular maps of the reorganization of genome-nuclear lamina interactions during differentiation

The three-dimensional organization of chromosomes within the nucleus and its dynamics during differentiation are largely unknown. To visualize this process in molecular detail, we generated high-resolution maps of genome-nuclear lamina interactions during subsequent differentiation of mouse embryoni...

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Published in:Molecular cell Vol. 38; no. 4; p. 603
Main Authors: Peric-Hupkes, Daan, Meuleman, Wouter, Pagie, Ludo, Bruggeman, Sophia W M, Solovei, Irina, Brugman, Wim, Gräf, Stefan, Flicek, Paul, Kerkhoven, Ron M, van Lohuizen, Maarten, Reinders, Marcel, Wessels, Lodewyk, van Steensel, Bas
Format: Journal Article
Language:English
Published: United States 28.05.2010
Subjects:
Animals
Astrocytes - cytology
Cell Differentiation
Cell Lineage
Chromosome Positioning
Drosophila
Embryonic Stem Cells - cytology
Genome
Humans
Mice
Neurons - cytology
Nuclear Lamina - metabolism
ISSN:1097-4164, 1097-4164
Online Access:Get more information
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Summary:The three-dimensional organization of chromosomes within the nucleus and its dynamics during differentiation are largely unknown. To visualize this process in molecular detail, we generated high-resolution maps of genome-nuclear lamina interactions during subsequent differentiation of mouse embryonic stem cells via lineage-committed neural precursor cells into terminally differentiated astrocytes. This reveals that a basal chromosome architecture present in embryonic stem cells is cumulatively altered at hundreds of sites during lineage commitment and subsequent terminal differentiation. This remodeling involves both individual transcription units and multigene regions and affects many genes that determine cellular identity. Often, genes that move away from the lamina are concomitantly activated; many others, however, remain inactive yet become unlocked for activation in a next differentiation step. These results suggest that lamina-genome interactions are widely involved in the control of gene expression programs during lineage commitment and terminal differentiation.
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ISSN:1097-4164
1097-4164
DOI:10.1016/j.molcel.2010.03.016