Differential subcompartmentation of terminal glycosylation in the Golgi apparatus of intestinal absorptive and goblet cells

Two terminal glycosyltransferases, a sialyltransferase and the blood group A alpha 1,3 N-acetylgalactosaminyltransferase, were found to exhibit differential subcompartmentation in the Golgi apparatus of intestinal goblet and absorptive cells. As expected from their role in terminal glycosylation, th...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:The Journal of biological chemistry Jg. 261; H. 30; S. 14307
Hauptverfasser: Roth, J, Taatjes, D J, Weinstein, J, Paulson, J C, Greenwell, P, Watkins, W M
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 25.10.1986
Schlagworte:
ABO Blood-Group System
Animals
Cell Compartmentation
Galactosyltransferases - analysis
Glycosylation
Golgi Apparatus - enzymology
Golgi Apparatus - ultrastructure
Histocytochemistry
Immunologic Techniques
Intestines - ultrastructure
Microscopy, Electron
N-Acetylgalactosaminyltransferases
Rats
Sialic Acids - analysis
Sialyltransferases - analysis
ISSN:0021-9258
Online-Zugang:Weitere Angaben
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Two terminal glycosyltransferases, a sialyltransferase and the blood group A alpha 1,3 N-acetylgalactosaminyltransferase, were found to exhibit differential subcompartmentation in the Golgi apparatus of intestinal goblet and absorptive cells. As expected from their role in terminal glycosylation, the two glycosyltransferases and their products, sialic acid residues and blood group A substance, were localized in the trans cisternae of the Golgi apparatus of goblet cells. In contrast, however, they were found throughout the Golgi apparatus stack of adjacent absorptive cells, with the exception of the fenestrated first cis cisterna. The results are in contrast to the general view that enzymes in the glycosylation pathway are arranged in a cis to trans gradient across the Golgi apparatus and that such polarized distributions may instead be cell type-specific.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0021-9258
DOI:10.1016/s0021-9258(18)67019-x