Efficient production of astaxanthin in Yarrowia lipolytica through metabolic and enzyme engineering

Astaxanthin is a natural red carotenoid, commonly used as an additive in the pharmaceutical industry and as a nutritional supplement owing to its notable antioxidant benefits. However, a complex biosynthetic pathway poses a challenge to de novo biosynthesis of astaxanthin. Here, Yarrowia lipolytica...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Synthetic and systems biotechnology Jg. 10; H. 3; S. 737 - 750
Hauptverfasser: Abdullah, Chalak Najat, Liu, Mengsu, Chen, Qihang, Gao, Song, Zhang, Changtai, Liu, Shike, Zhou, Jingwen
Format: Journal Article
Sprache:Englisch
Veröffentlicht: China Elsevier B.V 01.09.2025
KeAi Publishing Communications Ltd
KeAi Publishing
KeAi Communications Co., Ltd
Schlagworte:
Algae
Antioxidants
Astaxanthin
Bacteria
Batch culture
Biosynthesis
Carotene
Carotenoids
Catalytic activity
CRISPR
Dietary supplements
E coli
Enzyme engineering
Enzymes
Fermentation
Genes
Genomes
Glucose
Metabolic engineering
Modular enzyme assembly
Organelles
Original
Pharmaceutical industry
Plasmids
Productivity
Subcellular organelles
Yarrowia lipolytica
Yeast
β-Carotene
China
Shanghai China
Modular enzyme assembly
Subcellular organelles
Enzyme engineering
Astaxanthin
Yarrowia lipolytica
ISSN:2405-805X, 2405-805X
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Astaxanthin is a natural red carotenoid, commonly used as an additive in the pharmaceutical industry and as a nutritional supplement owing to its notable antioxidant benefits. However, a complex biosynthetic pathway poses a challenge to de novo biosynthesis of astaxanthin. Here, Yarrowia lipolytica was engineered through multiple strategies for high level production of astaxanthin using a cheap mineral medium. For the production of β-carotene, a platform strain was constructed in which 411.7 mg/L of β-carotene was produced at a shake-flask level. Integration of algal β-carotene ketolase and β-carotene hydroxylase led to the production of 12.3 mg/L of astaxanthin. Furthermore, construction of HpBKT and HpCrtZ as a single enzyme complex along with the enhanced catalytic activity of the enzymes led to the accumulation of 41.0 mg/L of astaxanthin. Iterative gene integration into the genome and direction of the astaxanthin production pathway into sub-organelles substantially increased astaxanthin production (172.1 mg/L). Finally, restoration of the auxotrophic markers and medium optimization further improved astaxanthin production to 237.3 mg/L. The aforementioned approaches were employed in fed-batch fermentation to produce 2820 mg/L of astaxanthin (229-fold improvement regarding the starter strain), with an average productivity of 434 mg/L/d and a yield of 5.6 mg/g glucose, which is the highest reported productivity in Y. lipolytica. [Display omitted]
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ISSN:2405-805X
2405-805X
DOI:10.1016/j.synbio.2025.02.014