Validation of RNAi Silencing Efficiency Using Gene Array Data shows 18.5% Failure Rate across 429 Independent Experiments

No independent cross-validation of success rate for studies utilizing small interfering RNA (siRNA) for gene silencing has been completed before. To assess the influence of experimental parameters like cell line, transfection technique, validation method, and type of control, we have to validate the...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Molecular therapy. Nucleic acids Jg. 5; H. 9; S. e366
Hauptverfasser:	Munkácsy, Gyöngyi, Sztupinszki, Zsófia, Herman, Péter, Bán, Bence, Pénzváltó, Zsófia, Szarvas, Nóra, Győrffy, Balázs
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States Elsevier Inc 2016 Elsevier Limited Nature Publishing Group Elsevier
Schlagworte:	cancer cell line Experiments gene silencing Gene therapy microarrays Original Ribonucleic acid RNA RNA interference transfection gene silencing cancer RNA interference cell line transfection microarrays
ISSN:	2162-2531, 2162-2531
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Abstract	No independent cross-validation of success rate for studies utilizing small interfering RNA (siRNA) for gene silencing has been completed before. To assess the influence of experimental parameters like cell line, transfection technique, validation method, and type of control, we have to validate these in a large set of studies. We utilized gene chip data published for siRNA experiments to assess success rate and to compare methods used in these experiments. We searched NCBI GEO for samples with whole transcriptome analysis before and after gene silencing and evaluated the efficiency for the target and off-target genes using the array-based expression data. Wilcoxon signed-rank test was used to assess silencing efficacy and Kruskal–Wallis tests and Spearman rank correlation were used to evaluate study parameters. All together 1,643 samples representing 429 experiments published in 207 studies were evaluated. The fold change (FC) of down-regulation of the target gene was above 0.7 in 18.5% and was above 0.5 in 38.7% of experiments. Silencing efficiency was lowest in MCF7 and highest in SW480 cells (FC = 0.59 and FC = 0.30, respectively, P = 9.3E−06). Studies utilizing Western blot for validation performed better than those with quantitative polymerase chain reaction (qPCR) or microarray (FC = 0.43, FC = 0.47, and FC = 0.55, respectively, P = 2.8E−04). There was no correlation between type of control, transfection method, publication year, and silencing efficiency. Although gene silencing is a robust feature successfully cross-validated in the majority of experiments, efficiency remained insufficient in a significant proportion of studies. Selection of cell line model and validation method had the highest influence on silencing proficiency.
AbstractList	No independent cross-validation of success rate for studies utilizing small interfering RNA (siRNA) for gene silencing has been completed before. To assess the influence of experimental parameters like cell line, transfection technique, validation method, and type of control, we have to validate these in a large set of studies. We utilized gene chip data published for siRNA experiments to assess success rate and to compare methods used in these experiments. We searched NCBI GEO for samples with whole transcriptome analysis before and after gene silencing and evaluated the efficiency for the target and off-target genes using the array-based expression data. Wilcoxon signed-rank test was used to assess silencing efficacy and Kruskal–Wallis tests and Spearman rank correlation were used to evaluate study parameters. All together 1,643 samples representing 429 experiments published in 207 studies were evaluated. The fold change (FC) of down-regulation of the target gene was above 0.7 in 18.5% and was above 0.5 in 38.7% of experiments. Silencing efficiency was lowest in MCF7 and highest in SW480 cells (FC = 0.59 and FC = 0.30, respectively, P = 9.3E−06). Studies utilizing Western blot for validation performed better than those with quantitative polymerase chain reaction (qPCR) or microarray (FC = 0.43, FC = 0.47, and FC = 0.55, respectively, P = 2.8E−04). There was no correlation between type of control, transfection method, publication year, and silencing efficiency. Although gene silencing is a robust feature successfully cross-validated in the majority of experiments, efficiency remained insufficient in a significant proportion of studies. Selection of cell line model and validation method had the highest influence on silencing proficiency. No independent cross-validation of success rate for studies utilizing small interfering RNA (siRNA) for gene silencing has been completed before. To assess the influence of experimental parameters like cell line, transfection technique, validation method, and type of control, we have to validate these in a large set of studies. We utilized gene chip data published for siRNA experiments to assess success rate and to compare methods used in these experiments. We searched NCBI GEO for samples with whole transcriptome analysis before and after gene silencing and evaluated the efficiency for the target and off-target genes using the array-based expression data. Wilcoxon signed-rank test was used to assess silencing efficacy and Kruskal-Wallis tests and Spearman rank correlation were used to evaluate study parameters. All together 1,643 samples representing 429 experiments published in 207 studies were evaluated. The fold change (FC) of down-regulation of the target gene was above 0.7 in 18.5% and was above 0.5 in 38.7% of experiments. Silencing efficiency was lowest in MCF7 and highest in SW480 cells (FC = 0.59 and FC = 0.30, respectively, P = 9.3E-06). Studies utilizing Western blot for validation performed better than those with quantitative polymerase chain reaction (qPCR) or microarray (FC = 0.43, FC = 0.47, and FC = 0.55, respectively, P = 2.8E-04). There was no correlation between type of control, transfection method, publication year, and silencing efficiency. Although gene silencing is a robust feature successfully cross-validated in the majority of experiments, efficiency remained insufficient in a significant proportion of studies. Selection of cell line model and validation method had the highest influence on silencing proficiency. No independent cross-validation of success rate for studies utilizing small interfering RNA (siRNA) for gene silencing has been completed before. To assess the inuence of experimental parameters like cell line, transfection technique, validation method, and type of control, we have to validate these in a large set of studies. We utilized gene chip data published for siRNA experiments to assess success rate and to compare methods used in these experiments. We searched NCBI GEO for samples with whole transcriptome analysis before and after gene silencing and evaluated the efciency for the target and off-target genes using the array-based expression data. Wilcoxon signed-rank test was used to assess silencing efcacy and KruskalWallis tests and Spearman rank correlation were used to evaluate study parameters. All together 1,643 samples representing 429 experiments published in 207 studies were evaluated. The fold change (FC) of down-regulation of the target gene was above 0.7 in 18.5% and was above 0.5 in 38.7% of experiments. Silencing efciency was lowest in MCF7 and highest in SW480 cells (FC = 0.59 and FC = 0.30, respectively, P = 9.3E06). Studies utilizing Western blot for validation performed better than those with quantitative polymerase chain reaction (qPCR) or microarray (FC = 0.43, FC = 0.47, and FC = 0.55, respectively, P = 2.8E04). There was no correlation between type of control, transfection method, publication year, and silencing efciency. Although gene silencing is a robust feature successfully cross-validated in the majority of experiments, efciency remained insufcient in a signicant proportion of studies. Selection of cell line model and validation method had the highest inuence on silencing prociency. Molecular TherapyNucleic Acids (2016) 5, e366; doi:10.1038/mtna.2016.66; published online 27 September 2016Subject Category: siRNA, shRNAs and miRNAS
ArticleNumber	e366
Author	Győrffy, Balázs Sztupinszki, Zsófia Szarvas, Nóra Herman, Péter Pénzváltó, Zsófia Munkácsy, Gyöngyi Bán, Bence
Author_xml	– sequence: 1 givenname: Gyöngyi surname: Munkácsy fullname: Munkácsy, Gyöngyi organization: MTA TTK Lendület Cancer Biomarker Research Group, Budapest, Hungary – sequence: 2 givenname: Zsófia surname: Sztupinszki fullname: Sztupinszki, Zsófia organization: Second Department of Pediatrics, Semmelweis University, Budapest, Hungary – sequence: 3 givenname: Péter surname: Herman fullname: Herman, Péter organization: MTA TTK Lendület Cancer Biomarker Research Group, Budapest, Hungary – sequence: 4 givenname: Bence surname: Bán fullname: Bán, Bence organization: Second Department of Pediatrics, Semmelweis University, Budapest, Hungary – sequence: 5 givenname: Zsófia surname: Pénzváltó fullname: Pénzváltó, Zsófia organization: MTA TTK Lendület Cancer Biomarker Research Group, Budapest, Hungary – sequence: 6 givenname: Nóra surname: Szarvas fullname: Szarvas, Nóra organization: MTA TTK Lendület Cancer Biomarker Research Group, Budapest, Hungary – sequence: 7 givenname: Balázs surname: Győrffy fullname: Győrffy, Balázs email: gyorffy.balazs@ttk.mta.hu organization: MTA TTK Lendület Cancer Biomarker Research Group, Budapest, Hungary
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/27673562$$D View this record in MEDLINE/PubMed
BookMark	eNqNkktvEzEUhUeoiJbSHWtkCSGxIMH2-DHeIEUlLZEqkAplazn2ndTRxA72pJB_jycpVVuBhBd-fvf42vc8rw5CDFBVLwkeE1w371d9MGOKiRgL8aQ6okTQEeU1Obg3P6xOcl7i0gQmVNBn1SGVQtZc0KNq-9103pnex4Biiy4_Tzz66jsI1ocFmratt74stugqDxvnEABNUjJb9NH0BuXr-DMj0oz5G3RmfLdJgC5ND8jYFHNGjCo0Cw7WULrQo-mvNSS_KtP8onrami7Dye14XF2dTb-dfhpdfDmfnU4uRpbzph_ZOcUWDEjJsBFKSOVa2TZKOWka5Ry0jRGCWAwFELZuKefU8taBNaAcrY-r2V7XRbPU63K7SVsdjde7jZgW2qTe2w40MYzaefm31szZvJGK1a4GwaVjdQ2cFK0Pe631Zr4CZ8s7kukeiD48Cf5aL-KN5pgLpXAReHsrkOKPDeRer3y20HUmQNxkTRomGBFEiv9AKeNMSKYK-voRuoybFMqvDhRntVT1kPyr-8nfZf3HDAV4twd2tUvQ3iEE68FuerCbHuymxZAgfYRb3--cVJ7uu38FiX0QlJLfeEg67wwGziewfamJ_3vgb-JQ6mQ
CitedBy_id	crossref_primary_10_1016_j_jtbi_2022_111055 crossref_primary_10_1016_j_jgg_2017_09_003 crossref_primary_10_1038_s41598_018_36122_8 crossref_primary_10_3389_fonc_2022_912942 crossref_primary_10_1371_journal_pone_0185943 crossref_primary_10_1007_s11103_017_0602_z crossref_primary_10_1038_s12276_019_0233_3
Cites_doi	10.1038/nrd3010 10.1038/nbt831 10.1038/nbt1239 10.1371/journal.pone.0005645 10.1261/rna.30706 10.1186/1471-2105-12-474 10.1152/ajplung.zh5-6122-retr.2012 10.1073/pnas.1111383108 10.3892/mmr.2015.4107 10.18632/oncotarget.4269 10.2174/138920210791110988 10.1038/nature08956 10.1016/S0092-8674(00)80620-0 10.1007/978-1-62703-119-6_3 10.1556/oh.2007.28199 10.1038/nbt1151 10.1093/nar/gks797 10.1186/1758-907X-1-14 10.1093/nar/gkq1039 10.1186/s12864-015-2267-9 10.1186/1471-2105-10-392 10.1093/nar/30.8.1757 10.1101/gr.082701.108 10.1038/sj.bjc.6606002 10.1093/embo-reports/kve230 10.1038/sj.bjc.6605478 10.1158/2159-8290.CD-12-0429 10.1016/j.cell.2005.07.031 10.1038/sj.gt.3302641 10.1007/s10549-011-1676-y 10.1038/468487a
ContentType	Journal Article
Copyright	2016 Official journal of the American Society of Gene & Cell Therapy Copyright Nature Publishing Group Sep 2016 Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy 2016 Official journal of the American Society of Gene & Cell Therapy
Copyright_xml	– notice: 2016 Official journal of the American Society of Gene & Cell Therapy – notice: Copyright Nature Publishing Group Sep 2016 – notice: Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy 2016 Official journal of the American Society of Gene & Cell Therapy
DBID	6I. AAFTH AAYXX CITATION NPM 3V. 7X7 7XB 88A 88I 8FE 8FH 8FI 8FJ 8FK ABUWG AFKRA AZQEC BBNVY BENPR BHPHI CCPQU DWQXO FYUFA GHDGH GNUQQ HCIFZ K9. LK8 M0S M2P M7P PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI PRINS Q9U 7X8 7TM 5PM DOA
DOI	10.1038/mtna.2016.66
DatabaseName	ScienceDirect Open Access Titles Elsevier:ScienceDirect:Open Access CrossRef PubMed ProQuest Central (Corporate) Health & Medical Collection ProQuest Central (purchase pre-March 2016) Biology Database (Alumni Edition) Science Database (Alumni Edition) ProQuest SciTech Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni) ProQuest Central UK/Ireland ProQuest Central Essentials Biological Science Collection ProQuest Central Natural Science Collection ProQuest One Community College ProQuest Central Korea Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) ProQuest Biological Science Collection Health & Medical Collection (Alumni Edition) Science Database Biological Science Database ProQuest Central Premium ProQuest One Academic (New) Publicly Available Content Database ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic (retired) ProQuest One Academic UKI Edition ProQuest Central China ProQuest Central Basic MEDLINE - Academic Nucleic Acids Abstracts PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals
DatabaseTitle	CrossRef PubMed Publicly Available Content Database ProQuest Central Student ProQuest One Academic Middle East (New) ProQuest Central Essentials ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest One Health & Nursing ProQuest Natural Science Collection ProQuest Central China ProQuest Biology Journals (Alumni Edition) ProQuest Central ProQuest One Applied & Life Sciences ProQuest Health & Medical Research Collection Health Research Premium Collection Health and Medicine Complete (Alumni Edition) Natural Science Collection ProQuest Central Korea Biological Science Collection ProQuest Central (New) ProQuest Science Journals (Alumni Edition) ProQuest Biological Science Collection ProQuest Central Basic ProQuest Science Journals ProQuest One Academic Eastern Edition ProQuest Hospital Collection Health Research Premium Collection (Alumni) Biological Science Database ProQuest SciTech Collection ProQuest Hospital Collection (Alumni) ProQuest Health & Medical Complete ProQuest One Academic UKI Edition ProQuest One Academic ProQuest One Academic (New) ProQuest Central (Alumni) MEDLINE - Academic Nucleic Acids Abstracts
DatabaseTitleList	Nucleic Acids Abstracts PubMed Publicly Available Content Database MEDLINE - Academic
Database_xml	– sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: PIMPY name: Publicly Available Content Database url: http://search.proquest.com/publiccontent sourceTypes: Aggregation Database
DeliveryMethod	fulltext_linktorsrc
Discipline	Anatomy & Physiology
EISSN	2162-2531
EndPage	e366
ExternalDocumentID	oai_doaj_org_article_1a42cb162fab4b87943d3e657d433e51 PMC5056990 4202808011 27673562 10_1038_mtna_2016_66 S2162253117300859
Genre	Journal Article
GroupedDBID	0R~ 0SF 3V. 53G 5VS 6I. 7X7 88A 88I 8FE 8FH 8FI 8FJ AACTN AAEDW AAFTH AALRI AAXUO ABMAC ABUWG ACGFS ADBBV ADRAZ AEXQZ AFKRA AFTJW AITUG ALMA_UNASSIGNED_HOLDINGS AMRAJ AOIJS AZQEC BBNVY BCNDV BENPR BHPHI BPHCQ BVXVI CCPQU DIK DWQXO EBS EJD FDB FYUFA GNUQQ GROUPED_DOAJ HCIFZ HMCUK HYE IPNFZ KQ8 LK8 M0L M2P M41 M48 M7P M~E NCXOZ O9- OK1 PIMPY PQQKQ PROAC RIG RNTTT ROL RPM SSZ UKHRP AAMRU AAYWO AAYXX ADVLN AFFHD APXCP CITATION PHGZM PHGZT PQGLB ALIPV NPM 7XB 8FK K9. PJZUB PKEHL PPXIY PQEST PQUKI PRINS Q9U 7X8 PUEGO 7TM 5PM
ID	FETCH-LOGICAL-c558t-cb20ceae7740a69679df7f899d7a89ddef8a661c0e7406c3f2552c5fdecae9d23
IEDL.DBID	DOA
ISICitedReferencesCount	8
ISICitedReferencesURI	http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000395780600001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN	2162-2531
IngestDate	Fri Oct 03 12:50:48 EDT 2025 Tue Nov 04 01:53:42 EST 2025 Thu Oct 02 04:37:21 EDT 2025 Wed Oct 01 14:44:17 EDT 2025 Tue Oct 07 06:58:01 EDT 2025 Thu Apr 03 07:07:23 EDT 2025 Wed Nov 05 20:51:53 EST 2025 Tue Nov 18 21:31:37 EST 2025 Fri Feb 23 02:40:32 EST 2024
IsDoiOpenAccess	true
IsOpenAccess	true
IsPeerReviewed	true
IsScholarly	true
Issue	9
Keywords	gene silencing cancer RNA interference cell line transfection microarrays
Language	English
License	http://creativecommons.org/licenses/by-nc-nd/4.0 This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c558t-cb20ceae7740a69679df7f899d7a89ddef8a661c0e7406c3f2552c5fdecae9d23
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 The first two authors contributed equally to this work.
OpenAccessLink	https://doaj.org/article/1a42cb162fab4b87943d3e657d433e51
PMID	27673562
PQID	1825437931
PQPubID	2041944
ParticipantIDs	doaj_primary_oai_doaj_org_article_1a42cb162fab4b87943d3e657d433e51 pubmedcentral_primary_oai_pubmedcentral_nih_gov_5056990 proquest_miscellaneous_1846416176 proquest_miscellaneous_1824546749 proquest_journals_1825437931 pubmed_primary_27673562 crossref_primary_10_1038_mtna_2016_66 crossref_citationtrail_10_1038_mtna_2016_66 elsevier_sciencedirect_doi_10_1038_mtna_2016_66
PublicationCentury	2000
PublicationDate	2016-00-00
PublicationDateYYYYMMDD	2016-01-01
PublicationDate_xml	– year: 2016 text: 2016-00-00
PublicationDecade	2010
PublicationPlace	United States
PublicationPlace_xml	– name: United States – name: Milwaukee
PublicationTitle	Molecular therapy. Nucleic acids
PublicationTitleAlternate	Mol Ther Nucleic Acids
PublicationYear	2016
Publisher	Elsevier Inc Elsevier Limited Nature Publishing Group Elsevier
Publisher_xml	– name: Elsevier Inc – name: Elsevier Limited – name: Nature Publishing Group – name: Elsevier
References	Roos (bib17) 2011; 104 Friedman, Farh, Burge, Bartel (bib2) 2009; 19 Jackson, Bartz, Schelter, Kobayashi, Burchard, Mao (bib8) 2003; 21 Ledford (bib14) 2010; 468 Boudreau, Spengler, Hylock, Kusenda, Davis, Eichmann (bib11) 2013; 41 Vaishnaw, Gollob, Gamba-Vitalo, Hutabarat, Sah, Meyers (bib26) 2010; 1 (bib16) 2012; 302 Munkácsy, Tulassay, Gyorffy (bib4) 2007; 148 (bib15) 2015; 12 Raof, Rajamani, Chu, Gurav, Johnson, LoGerfo (bib22) 2016; 17 Lipardi, Paterson (bib18) 2011; 108 Davis, Zuckerman, Choi, Seligson, Tolcher, Alabi (bib27) 2010; 464 Munkácsy, Abdul-Ghani, Mihály, Tegze, Tchernitsa, Surowiak (bib19) 2010; 102 Shi (bib21) 2006; 24 Naito, Yoshimura, Morishita, Ui-Tei (bib12) 2009; 10 Shen, Samul, Silva, Akiyama, Liu, Saishin (bib25) 2006; 13 Li, Birkbak, Gyorffy, Szallasi, Eklund (bib20) 2011; 12 Tabernero, Shapiro, LoRusso, Cervantes, Schwartz, Weiss (bib28) 2013; 3 Gyorffy, Molnar, Lage, Szallasi, Eklund (bib29) 2009; 4 Holen, Amarzguioui, Wiiger, Babaie, Prydz (bib6) 2002; 30 Bagga, Bracht, Hunter, Massirer, Holtz, Eachus (bib3) 2005; 122 Zamore, Tuschl, Sharp, Bartel (bib5) 2000; 101 Patzel, Rutz, Dietrich, Köberle, Scheffold, Kaufmann (bib9) 2005; 23 Naito, Ui-Tei (bib10) 2013; 942 Jackson, Burchard, Leake, Reynolds, Schelter, Guo (bib13) 2006; 12 Li, Liu, Yang, Liu, Wang, Dong (bib24) 2015; 6 Győrffy, Benke, Lánczky, Balázs, Szállási, Timár (bib30) 2012; 132 Jackson, Linsley (bib7) 2010; 9 Cerami, Gross, Demir, Rodchenkov, Babur, Anwar (bib31) 2011; 39 Boettcher, Hoheisel (bib23) 2010; 11 Mattick (bib1) 2001; 2 Boudreau (10.1038/mtna.2016.66_bib11) 2013; 41 (10.1038/mtna.2016.66_bib16) 2012; 302 Shen (10.1038/mtna.2016.66_bib25) 2006; 13 Shi (10.1038/mtna.2016.66_bib21) 2006; 24 (10.1038/mtna.2016.66_bib15) 2015; 12 Munkácsy (10.1038/mtna.2016.66_bib19) 2010; 102 Jackson (10.1038/mtna.2016.66_bib13) 2006; 12 Li (10.1038/mtna.2016.66_bib20) 2011; 12 Gyorffy (10.1038/mtna.2016.66_bib29) 2009; 4 Jackson (10.1038/mtna.2016.66_bib8) 2003; 21 Bagga (10.1038/mtna.2016.66_bib3) 2005; 122 Zamore (10.1038/mtna.2016.66_bib5) 2000; 101 Cerami (10.1038/mtna.2016.66_bib31) 2011; 39 Lipardi (10.1038/mtna.2016.66_bib18) 2011; 108 Vaishnaw (10.1038/mtna.2016.66_bib26) 2010; 1 Mattick (10.1038/mtna.2016.66_bib1) 2001; 2 Roos (10.1038/mtna.2016.66_bib17) 2011; 104 Friedman (10.1038/mtna.2016.66_bib2) 2009; 19 Munkácsy (10.1038/mtna.2016.66_bib4) 2007; 148 Davis (10.1038/mtna.2016.66_bib27) 2010; 464 Tabernero (10.1038/mtna.2016.66_bib28) 2013; 3 Naito (10.1038/mtna.2016.66_bib12) 2009; 10 Boettcher (10.1038/mtna.2016.66_bib23) 2010; 11 Ledford (10.1038/mtna.2016.66_bib14) 2010; 468 Li (10.1038/mtna.2016.66_bib24) 2015; 6 Jackson (10.1038/mtna.2016.66_bib7) 2010; 9 Patzel (10.1038/mtna.2016.66_bib9) 2005; 23 Holen (10.1038/mtna.2016.66_bib6) 2002; 30 Győrffy (10.1038/mtna.2016.66_bib30) 2012; 132 Naito (10.1038/mtna.2016.66_bib10) 2013; 942 Raof (10.1038/mtna.2016.66_bib22) 2016; 17 11937629 - Nucleic Acids Res. 2002 Apr 15;30(8):1757-66 22172014 - BMC Bioinformatics. 2011 Dec 15;12:474 23358650 - Cancer Discov. 2013 Apr;3(4):406-17 19461970 - PLoS One. 2009 May 21;4(5):e5645 16964229 - Nat Biotechnol. 2006 Sep;24(9):1151-61 21071392 - Nucleic Acids Res. 2011 Jan;39(Database issue):D685-90 16682562 - RNA. 2006 Jul;12(7):1197-205 21773767 - Breast Cancer Res Treat. 2012 Apr;132(3):1025-34 16258545 - Nat Biotechnol. 2005 Nov;23(11):1440-4 22941647 - Nucleic Acids Res. 2013 Jan 7;41(1):e9 18003582 - Orv Hetil. 2007 Nov 25;148(47):2235-40 21037854 - Curr Genomics. 2010 May;11(3):162-7 26238072 - Mol Med Rep. 2015 Oct;12(4):5601 20305636 - Nature. 2010 Apr 15;464(7291):1067-70 18955434 - Genome Res. 2009 Jan;19(1):92-105 11713189 - EMBO Rep. 2001 Nov;2(11):986-91 21821790 - Proc Natl Acad Sci U S A. 2011 Sep 6;108(36):15010 23027045 - Methods Mol Biol. 2013;942:57-68 19948054 - BMC Bioinformatics. 2009 Nov 30;10:392 20615220 - Silence. 2010 Jul 08;1(1):14 21107398 - Nature. 2010 Nov 25;468(7323):487 22549970 - Am J Physiol Lung Cell Mol Physiol. 2012 May 1;302(9):L976 16195704 - Gene Ther. 2006 Feb;13(3):225-34 21206499 - Br J Cancer. 2011 Jan 4;104(1):227 20043028 - Nat Rev Drug Discov. 2010 Jan;9(1):57-67 26728506 - BMC Genomics. 2016 Jan 05;17 :20 26057633 - Oncotarget. 2015 Aug 28;6(25):21603-13 10778853 - Cell. 2000 Mar 31;101(1):25-33 16122423 - Cell. 2005 Aug 26;122(4):553-63 20010949 - Br J Cancer. 2010 Jan 19;102(2):361-8 12754523 - Nat Biotechnol. 2003 Jun;21(6):635-7
References_xml	– volume: 12 start-page: 1197 year: 2006 end-page: 1205 ident: bib13 article-title: Position-specific chemical modification of siRNAs reduces “off-target” transcript silencing publication-title: RNA – volume: 2 start-page: 986 year: 2001 end-page: 991 ident: bib1 article-title: Non-coding RNAs: the architects of eukaryotic complexity publication-title: EMBO Rep – volume: 12 start-page: 474 year: 2011 ident: bib20 article-title: Jetset: selecting the optimal microarray probe set to represent a gene publication-title: BMC Bioinformatics – volume: 9 start-page: 57 year: 2010 end-page: 67 ident: bib7 article-title: Recognizing and avoiding siRNA off-target effects for target identification and therapeutic application publication-title: Nat Rev Drug Discov – volume: 10 start-page: 392 year: 2009 ident: bib12 article-title: siDirect 2.0: updated software for designing functional siRNA with reduced seed-dependent off-target effect publication-title: BMC Bioinformatics – volume: 11 start-page: 162 year: 2010 end-page: 167 ident: bib23 article-title: Pooled RNAi screens–technical and biological aspects publication-title: Curr Genomics – volume: 39 start-page: D685 year: 2011 end-page: D690 ident: bib31 article-title: Pathway commons, a web resource for biological pathway data publication-title: Nucleic Acids Res – volume: 1 start-page: 14 year: 2010 ident: bib26 article-title: A status report on RNAi therapeutics publication-title: Silence – volume: 132 start-page: 1025 year: 2012 end-page: 1034 ident: bib30 article-title: Recurrence Online: an online analysis tool to determine breast cancer recurrence and hormone receptor status using microarray data publication-title: Breast Cancer Res Treat – volume: 104 start-page: 227 year: 2011 ident: bib17 article-title: Retraction. Effect of the chemokine receptor CXCR7 on proliferation of carcinoma cells in vitro and in vivo publication-title: Br J Cancer – volume: 3 start-page: 406 year: 2013 end-page: 417 ident: bib28 article-title: First-in-humans trial of an RNA interference therapeutic targeting VEGF and KSP in cancer patients with liver involvement publication-title: Cancer Discov – volume: 148 start-page: 2235 year: 2007 end-page: 2240 ident: bib4 article-title: [RNA interference and its clinical applications] publication-title: Orv Hetil – volume: 468 start-page: 487 year: 2010 ident: bib14 article-title: Drug giants turn their backs on RNA interference publication-title: Nature – volume: 4 start-page: e5645 year: 2009 ident: bib29 article-title: Evaluation of microarray preprocessing algorithms based on concordance with RT-PCR in clinical samples publication-title: PLoS One – volume: 13 start-page: 225 year: 2006 end-page: 234 ident: bib25 article-title: Suppression of ocular neovascularization with siRNA targeting VEGF receptor 1 publication-title: Gene Ther – volume: 122 start-page: 553 year: 2005 end-page: 563 ident: bib3 article-title: Regulation by let-7 and lin-4 miRNAs results in target mRNA degradation publication-title: Cell – volume: 24 start-page: 1151 year: 2006 end-page: 1161 ident: bib21 article-title: The MicroArray Quality Control (MAQC) project shows inter- and intraplatform reproducibility of gene expression measurements publication-title: Nat Biotechnol – volume: 302 start-page: L976 year: 2012 ident: bib16 article-title: Retraction publication-title: American journal of physiology. Lung cellular and molecular physiology – volume: 102 start-page: 361 year: 2010 end-page: 368 ident: bib19 article-title: PSMB7 is associated with anthracycline resistance and is a prognostic biomarker in breast cancer publication-title: Br J Cancer – volume: 108 start-page: 15010 year: 2011 ident: bib18 article-title: Retraction for Lipardi and Paterson, “Identification of an RNA-dependent RNA polymerase in Drosophila involved in RNAi and transposon suppression” publication-title: Proc Natl Acad Sci USA – volume: 17 start-page: 20 year: 2016 ident: bib22 article-title: The effects of transfection reagent polyethyleneimine (PEI) and non-targeting control siRNAs on global gene expression in human aortic smooth muscle cells publication-title: BMC Genomics – volume: 23 start-page: 1440 year: 2005 end-page: 1444 ident: bib9 article-title: Design of siRNAs producing unstructured guide-RNAs results in improved RNA interference efficiency publication-title: Nat Biotechnol – volume: 12 start-page: 5601 year: 2015 ident: bib15 article-title: [Retraction] Interleukin-11 induces the expression of matrix metalloproteinase 13 in gastric cancer SCH cells partly via the PI3K-AKT and JAK-STAT3 pathways publication-title: Molecular Medicine Reports – volume: 6 start-page: 21603 year: 2015 end-page: 21613 ident: bib24 article-title: siRNAs with decreased off-target effect facilitate the identification of essential genes in cancer cells publication-title: Oncotarget – volume: 19 start-page: 92 year: 2009 end-page: 105 ident: bib2 article-title: Most mammalian mRNAs are conserved targets of microRNAs publication-title: Genome Res – volume: 30 start-page: 1757 year: 2002 end-page: 1766 ident: bib6 article-title: Positional effects of short interfering RNAs targeting the human coagulation trigger Tissue Factor publication-title: Nucleic Acids Res – volume: 101 start-page: 25 year: 2000 end-page: 33 ident: bib5 article-title: RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals publication-title: Cell – volume: 21 start-page: 635 year: 2003 end-page: 637 ident: bib8 article-title: Expression profiling reveals off-target gene regulation by RNAi publication-title: Nat Biotechnol – volume: 41 start-page: e9 year: 2013 ident: bib11 article-title: siSPOTR: a tool for designing highly specific and potent siRNAs for human and mouse publication-title: Nucleic Acids Res – volume: 464 start-page: 1067 year: 2010 end-page: 1070 ident: bib27 article-title: Evidence of RNAi in humans from systemically administered siRNA via targeted nanoparticles publication-title: Nature – volume: 942 start-page: 57 year: 2013 end-page: 68 ident: bib10 article-title: Designing functional siRNA with reduced off-target effects publication-title: Methods Mol Biol – volume: 9 start-page: 57 year: 2010 ident: 10.1038/mtna.2016.66_bib7 article-title: Recognizing and avoiding siRNA off-target effects for target identification and therapeutic application publication-title: Nat Rev Drug Discov doi: 10.1038/nrd3010 – volume: 21 start-page: 635 year: 2003 ident: 10.1038/mtna.2016.66_bib8 article-title: Expression profiling reveals off-target gene regulation by RNAi publication-title: Nat Biotechnol doi: 10.1038/nbt831 – volume: 24 start-page: 1151 year: 2006 ident: 10.1038/mtna.2016.66_bib21 article-title: The MicroArray Quality Control (MAQC) project shows inter- and intraplatform reproducibility of gene expression measurements publication-title: Nat Biotechnol doi: 10.1038/nbt1239 – volume: 4 start-page: e5645 year: 2009 ident: 10.1038/mtna.2016.66_bib29 article-title: Evaluation of microarray preprocessing algorithms based on concordance with RT-PCR in clinical samples publication-title: PLoS One doi: 10.1371/journal.pone.0005645 – volume: 12 start-page: 1197 year: 2006 ident: 10.1038/mtna.2016.66_bib13 article-title: Position-specific chemical modification of siRNAs reduces “off-target” transcript silencing publication-title: RNA doi: 10.1261/rna.30706 – volume: 12 start-page: 474 year: 2011 ident: 10.1038/mtna.2016.66_bib20 article-title: Jetset: selecting the optimal microarray probe set to represent a gene publication-title: BMC Bioinformatics doi: 10.1186/1471-2105-12-474 – volume: 302 start-page: L976 year: 2012 ident: 10.1038/mtna.2016.66_bib16 article-title: Retraction publication-title: American journal of physiology. Lung cellular and molecular physiology doi: 10.1152/ajplung.zh5-6122-retr.2012 – volume: 108 start-page: 15010 year: 2011 ident: 10.1038/mtna.2016.66_bib18 article-title: Retraction for Lipardi and Paterson, “Identification of an RNA-dependent RNA polymerase in Drosophila involved in RNAi and transposon suppression” publication-title: Proc Natl Acad Sci USA doi: 10.1073/pnas.1111383108 – volume: 12 start-page: 5601 year: 2015 ident: 10.1038/mtna.2016.66_bib15 article-title: [Retraction] Interleukin-11 induces the expression of matrix metalloproteinase 13 in gastric cancer SCH cells partly via the PI3K-AKT and JAK-STAT3 pathways publication-title: Molecular Medicine Reports doi: 10.3892/mmr.2015.4107 – volume: 6 start-page: 21603 year: 2015 ident: 10.1038/mtna.2016.66_bib24 article-title: siRNAs with decreased off-target effect facilitate the identification of essential genes in cancer cells publication-title: Oncotarget doi: 10.18632/oncotarget.4269 – volume: 11 start-page: 162 year: 2010 ident: 10.1038/mtna.2016.66_bib23 article-title: Pooled RNAi screens–technical and biological aspects publication-title: Curr Genomics doi: 10.2174/138920210791110988 – volume: 464 start-page: 1067 year: 2010 ident: 10.1038/mtna.2016.66_bib27 article-title: Evidence of RNAi in humans from systemically administered siRNA via targeted nanoparticles publication-title: Nature doi: 10.1038/nature08956 – volume: 101 start-page: 25 year: 2000 ident: 10.1038/mtna.2016.66_bib5 article-title: RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals publication-title: Cell doi: 10.1016/S0092-8674(00)80620-0 – volume: 942 start-page: 57 year: 2013 ident: 10.1038/mtna.2016.66_bib10 article-title: Designing functional siRNA with reduced off-target effects publication-title: Methods Mol Biol doi: 10.1007/978-1-62703-119-6_3 – volume: 148 start-page: 2235 year: 2007 ident: 10.1038/mtna.2016.66_bib4 article-title: [RNA interference and its clinical applications] publication-title: Orv Hetil doi: 10.1556/oh.2007.28199 – volume: 23 start-page: 1440 year: 2005 ident: 10.1038/mtna.2016.66_bib9 article-title: Design of siRNAs producing unstructured guide-RNAs results in improved RNA interference efficiency publication-title: Nat Biotechnol doi: 10.1038/nbt1151 – volume: 41 start-page: e9 year: 2013 ident: 10.1038/mtna.2016.66_bib11 article-title: siSPOTR: a tool for designing highly specific and potent siRNAs for human and mouse publication-title: Nucleic Acids Res doi: 10.1093/nar/gks797 – volume: 1 start-page: 14 year: 2010 ident: 10.1038/mtna.2016.66_bib26 article-title: A status report on RNAi therapeutics publication-title: Silence doi: 10.1186/1758-907X-1-14 – volume: 39 start-page: D685 issue: Database issue year: 2011 ident: 10.1038/mtna.2016.66_bib31 article-title: Pathway commons, a web resource for biological pathway data publication-title: Nucleic Acids Res doi: 10.1093/nar/gkq1039 – volume: 17 start-page: 20 year: 2016 ident: 10.1038/mtna.2016.66_bib22 article-title: The effects of transfection reagent polyethyleneimine (PEI) and non-targeting control siRNAs on global gene expression in human aortic smooth muscle cells publication-title: BMC Genomics doi: 10.1186/s12864-015-2267-9 – volume: 10 start-page: 392 year: 2009 ident: 10.1038/mtna.2016.66_bib12 article-title: siDirect 2.0: updated software for designing functional siRNA with reduced seed-dependent off-target effect publication-title: BMC Bioinformatics doi: 10.1186/1471-2105-10-392 – volume: 30 start-page: 1757 year: 2002 ident: 10.1038/mtna.2016.66_bib6 article-title: Positional effects of short interfering RNAs targeting the human coagulation trigger Tissue Factor publication-title: Nucleic Acids Res doi: 10.1093/nar/30.8.1757 – volume: 19 start-page: 92 year: 2009 ident: 10.1038/mtna.2016.66_bib2 article-title: Most mammalian mRNAs are conserved targets of microRNAs publication-title: Genome Res doi: 10.1101/gr.082701.108 – volume: 104 start-page: 227 year: 2011 ident: 10.1038/mtna.2016.66_bib17 article-title: Retraction. Effect of the chemokine receptor CXCR7 on proliferation of carcinoma cells in vitro and in vivo publication-title: Br J Cancer doi: 10.1038/sj.bjc.6606002 – volume: 2 start-page: 986 year: 2001 ident: 10.1038/mtna.2016.66_bib1 article-title: Non-coding RNAs: the architects of eukaryotic complexity publication-title: EMBO Rep doi: 10.1093/embo-reports/kve230 – volume: 102 start-page: 361 year: 2010 ident: 10.1038/mtna.2016.66_bib19 article-title: PSMB7 is associated with anthracycline resistance and is a prognostic biomarker in breast cancer publication-title: Br J Cancer doi: 10.1038/sj.bjc.6605478 – volume: 3 start-page: 406 year: 2013 ident: 10.1038/mtna.2016.66_bib28 article-title: First-in-humans trial of an RNA interference therapeutic targeting VEGF and KSP in cancer patients with liver involvement publication-title: Cancer Discov doi: 10.1158/2159-8290.CD-12-0429 – volume: 122 start-page: 553 year: 2005 ident: 10.1038/mtna.2016.66_bib3 article-title: Regulation by let-7 and lin-4 miRNAs results in target mRNA degradation publication-title: Cell doi: 10.1016/j.cell.2005.07.031 – volume: 13 start-page: 225 year: 2006 ident: 10.1038/mtna.2016.66_bib25 article-title: Suppression of ocular neovascularization with siRNA targeting VEGF receptor 1 publication-title: Gene Ther doi: 10.1038/sj.gt.3302641 – volume: 132 start-page: 1025 year: 2012 ident: 10.1038/mtna.2016.66_bib30 article-title: Recurrence Online: an online analysis tool to determine breast cancer recurrence and hormone receptor status using microarray data publication-title: Breast Cancer Res Treat doi: 10.1007/s10549-011-1676-y – volume: 468 start-page: 487 year: 2010 ident: 10.1038/mtna.2016.66_bib14 article-title: Drug giants turn their backs on RNA interference publication-title: Nature doi: 10.1038/468487a – reference: 20043028 - Nat Rev Drug Discov. 2010 Jan;9(1):57-67 – reference: 18955434 - Genome Res. 2009 Jan;19(1):92-105 – reference: 16964229 - Nat Biotechnol. 2006 Sep;24(9):1151-61 – reference: 16122423 - Cell. 2005 Aug 26;122(4):553-63 – reference: 16682562 - RNA. 2006 Jul;12(7):1197-205 – reference: 19461970 - PLoS One. 2009 May 21;4(5):e5645 – reference: 21773767 - Breast Cancer Res Treat. 2012 Apr;132(3):1025-34 – reference: 18003582 - Orv Hetil. 2007 Nov 25;148(47):2235-40 – reference: 21107398 - Nature. 2010 Nov 25;468(7323):487 – reference: 20010949 - Br J Cancer. 2010 Jan 19;102(2):361-8 – reference: 16195704 - Gene Ther. 2006 Feb;13(3):225-34 – reference: 10778853 - Cell. 2000 Mar 31;101(1):25-33 – reference: 16258545 - Nat Biotechnol. 2005 Nov;23(11):1440-4 – reference: 22941647 - Nucleic Acids Res. 2013 Jan 7;41(1):e9 – reference: 20615220 - Silence. 2010 Jul 08;1(1):14 – reference: 22549970 - Am J Physiol Lung Cell Mol Physiol. 2012 May 1;302(9):L976 – reference: 21071392 - Nucleic Acids Res. 2011 Jan;39(Database issue):D685-90 – reference: 23358650 - Cancer Discov. 2013 Apr;3(4):406-17 – reference: 26728506 - BMC Genomics. 2016 Jan 05;17 :20 – reference: 12754523 - Nat Biotechnol. 2003 Jun;21(6):635-7 – reference: 26238072 - Mol Med Rep. 2015 Oct;12(4):5601 – reference: 11713189 - EMBO Rep. 2001 Nov;2(11):986-91 – reference: 21037854 - Curr Genomics. 2010 May;11(3):162-7 – reference: 26057633 - Oncotarget. 2015 Aug 28;6(25):21603-13 – reference: 23027045 - Methods Mol Biol. 2013;942:57-68 – reference: 21821790 - Proc Natl Acad Sci U S A. 2011 Sep 6;108(36):15010 – reference: 21206499 - Br J Cancer. 2011 Jan 4;104(1):227 – reference: 11937629 - Nucleic Acids Res. 2002 Apr 15;30(8):1757-66 – reference: 20305636 - Nature. 2010 Apr 15;464(7291):1067-70 – reference: 22172014 - BMC Bioinformatics. 2011 Dec 15;12:474 – reference: 19948054 - BMC Bioinformatics. 2009 Nov 30;10:392
SSID	ssj0000601262
Score	2.0919573
Snippet	No independent cross-validation of success rate for studies utilizing small interfering RNA (siRNA) for gene silencing has been completed before. To assess the...
SourceID	doaj pubmedcentral proquest pubmed crossref elsevier
SourceType	Open Website Open Access Repository Aggregation Database Index Database Enrichment Source Publisher
StartPage	e366
SubjectTerms	cancer cell line Experiments gene silencing Gene therapy microarrays Original Ribonucleic acid RNA RNA interference transfection
SummonAdditionalLinks	– databaseName: Science Database dbid: M2P link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lb9QwELagcODCq1BSCjIS5YLSTeLEcU5ogV3BgVVVHuotcvygkWjSblLQ_ntmHG_KgtoL12TkxPbY8814_A0hL8FoCRlVLGTcXcmJVCi1lWGVABaRcaRtpFyxiXyxEMfHxaEPuHU-rXK9J7qNWrcKY-STGF0ZBtoUvzk7D7FqFJ6u-hIaN8ktQDYxpnR9Sg7HGAtyjSQ88fnuEROT075BsqGYHzhaxEtL5Aj7NwzSv4Dz77zJPwzR_N7_duE-ueshKJ0OOvOA3DDNQ7I9bcD9Pl3RV9Qlhbpo-zZZfQOcPpRdoq2lR4tpTT_XeFEJTB6dOf4JvLxJXeoBRRJraHgpV_S97CXtTtpfHY3FQbZP57LGFHh6BOiWSjcmFMwU_TgW4u3pbKw30D0iX-ezL-8-hL5aQ6iyTPShqpJIGWkAT0aSFzwvtM0tuHM6l6KAXdQKCWBARQYEuGIWnJlEZVYbJU2hE_aYbDVtY54QanURmUKk0ABLszwHn0wrCXbUmkoBogvI6_XMlcpTmWNFjR-lO1JnosR5LnGeS84Dsj9Knw0UHlfIvUUlGGWQeNs9aJffS7-Oy1imiapinlhZpZVAej3NDM9ynTJmsjggk7UKlR7DDNgEmqqv-OzeWltKv3905aWqBOTF-BpWPh7nyMa0F04mzbBYTHGdTMqdCwuf2RmUd-xfkvOcAfwNSL6h1hsDsPmmqU8cAznCZoAxu9f_-lNyB_s4BKz2yFa_vDDPyG31s6-75XO3VH8DptBKlQ priority: 102 providerName: ProQuest
Title	Validation of RNAi Silencing Efficiency Using Gene Array Data shows 18.5% Failure Rate across 429 Independent Experiments
URI	https://dx.doi.org/10.1038/mtna.2016.66 https://www.ncbi.nlm.nih.gov/pubmed/27673562 https://www.proquest.com/docview/1825437931 https://www.proquest.com/docview/1824546749 https://www.proquest.com/docview/1846416176 https://pubmed.ncbi.nlm.nih.gov/PMC5056990 https://doaj.org/article/1a42cb162fab4b87943d3e657d433e51
Volume	5
WOSCitedRecordID	wos000395780600001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
journalDatabaseRights	– providerCode: PRVAON databaseName: DOAJ Directory of Open Access Journals customDbUrl: eissn: 2162-2531 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000601262 issn: 2162-2531 databaseCode: DOA dateStart: 20120101 isFulltext: true titleUrlDefault: https://www.doaj.org/ providerName: Directory of Open Access Journals – providerCode: PRVHPJ databaseName: ROAD: Directory of Open Access Scholarly Resources customDbUrl: eissn: 2162-2531 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000601262 issn: 2162-2531 databaseCode: M~E dateStart: 20120101 isFulltext: true titleUrlDefault: https://road.issn.org providerName: ISSN International Centre – providerCode: PRVPQU databaseName: Biological Science Database customDbUrl: eissn: 2162-2531 dateEnd: 20191206 omitProxy: false ssIdentifier: ssj0000601262 issn: 2162-2531 databaseCode: M7P dateStart: 20120101 isFulltext: true titleUrlDefault: http://search.proquest.com/biologicalscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: Health & Medical Collection customDbUrl: eissn: 2162-2531 dateEnd: 20191206 omitProxy: false ssIdentifier: ssj0000601262 issn: 2162-2531 databaseCode: 7X7 dateStart: 20120101 isFulltext: true titleUrlDefault: https://search.proquest.com/healthcomplete providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Central customDbUrl: eissn: 2162-2531 dateEnd: 20191206 omitProxy: false ssIdentifier: ssj0000601262 issn: 2162-2531 databaseCode: BENPR dateStart: 20120101 isFulltext: true titleUrlDefault: https://www.proquest.com/central providerName: ProQuest – providerCode: PRVPQU databaseName: Publicly Available Content Database customDbUrl: eissn: 2162-2531 dateEnd: 20191206 omitProxy: false ssIdentifier: ssj0000601262 issn: 2162-2531 databaseCode: PIMPY dateStart: 20120101 isFulltext: true titleUrlDefault: http://search.proquest.com/publiccontent providerName: ProQuest – providerCode: PRVPQU databaseName: Science Database customDbUrl: eissn: 2162-2531 dateEnd: 20191206 omitProxy: false ssIdentifier: ssj0000601262 issn: 2162-2531 databaseCode: M2P dateStart: 20120101 isFulltext: true titleUrlDefault: https://search.proquest.com/sciencejournals providerName: ProQuest
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lj9MwELZglwMXBCyPwlIZieWCsnXsxHaOXWjFHraqykPlFDl-aCOxKWqyoP57xk4aWtDChYsPzdRJxpOZb5LxNwi9gqAlFSlYxHjYkkN0pIxTUUEBi6iYGEd0aDYhZjO5XGbznVZfviaspQduFTeKVUJ1EXPqVJEU0vOZGWZ5KkzCmA2bpykR2U4y1fpgcLyhmyiFv0YULK2reidMjq6aylMOxfw0kCP-ikeBtn8vLP0JO3-vntwJR9P76F6HI_G4vf4H6JatHqKjcQU59NUGv8ahsjO8Mj9Cm88AttveSXjl8GI2LvGH0u82griFJ4FEwu_AxKF-AHsmaph4rTb4nWoUri9XP2ocy9P0BE9V6evY8QIgKlbhljDEGnzed9Nt8KRvGlA_Qp-mk49v30ddy4VIp6lsIl1Qoq2yAAqJ4hkXmXHCQU5mhJIZuEInFUR0TSwIcM0cZCRUp85YrWxmKHuMDqpVZZ8i7ExGbCYTmIAlqRCQWBmtIBg6W2iAZQP0Zqv4XHd85L4txtc8fBdnMvfLlPtlyjkfoJNe-lvLw3GD3Jlfw17Gs2eHH8Cm8s6m8n_Z1ACNthaQd0CkBRgwVXnDaY-3hpJ3TqDOY599M3CAMOHL_jA8vv6bjKrs6jrIJKnv-JL9TSbhIQ-F0zxpba-_Pyq4YIBhB0jsWeWeAvaPVOVloBH32BewyLP_obHn6K7XRPtu6hgdNOtr-wLd0d-bsl4P0W2xFGGUQ3R4NpnNF8PwvMJ4Qed-FDAezs8v5l9-AidDRQM
linkProvider	Directory of Open Access Journals
linkToHtml	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Lb9QwELaqggQXXuWxUMBILBeUbuIkTnJAaKG7atWyqkpBvbmOY9NINCmblGr_FL-RGedRFtTeeuCajJzEmcc39vgbQl5D0Iqlm_qOz-2RHFc5MjPSSRlgEem5mXGVbTYRzWbx4WGyt0J-dWdhsKyy84nWUWelwjXykYepjA_a5L0__eFg1yjcXe1aaDRqsaMX55CyVe-2N-H_DhmbTg4-bjltVwFHhWFcOyplrtJSA-5xJU94lGQmMpB2ZJGME7B2E0sIWsrVIMCVbwB0MxWaTCupkwyJDsDl3wiQWQxLBdlev6aD3CaMs7a-3vXj0UldILmRxzcsDeNF5LMNApYC4L8A9-86zT8C3_Tu_zZl98idFmLTcWMT98mKLh6QtXEh6_JkQd9QW_RqdxPWyOIr5CFNWylaGro_G-f0c44HsSCk04nl18DDqdSWVlAk6YaB53JBN2UtaXVcnlfUizfCIZ3KHEv86T6gdyrtP6AQhul232i4ppO-n0L1kHy5lkl4RFaLstBPCDVZ4uokDmAAPwijCHLOTEnACUanChDrgLztNEWolqodO4Z8F7ZkwI8F6pVAvRKcD8iwlz5tKEoukfuAStfLILG4vVDOv4nWTwlPBkylHmdGpkEaI31g5mseRlng-zr0BmTUqaxoMVqDvWCo_JLHrnfaKVr_WIkL1RyQV_1t8Gy4XSULXZ5ZmSDEZjjJVTIBtyk6POZxYyz997GIRz7A-wGJlsxoaQKW7xT5sWVYx7QAYNrTq1_9Jbm1dfBpV-xuz3aekdv4vc3i3DpZredn-jm5qX7WeTV_Yd0EJUfXbWS_AbTsqnQ
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Validation+of+RNAi+Silencing+Efficiency+Using+Gene+Array+Data+shows+18.5%25+Failure+Rate+across+429+Independent+Experiments&rft.jtitle=Molecular+therapy.+Nucleic+acids&rft.au=Munk%C3%A1csy%2C+Gy%C3%B6ngyi&rft.au=Sztupinszki%2C+Zs%C3%B3fia&rft.au=Herman%2C+P%C3%A9ter&rft.au=B%C3%A1n%2C+Bence&rft.date=2016&rft.pub=Nature+Publishing+Group&rft.eissn=2162-2531&rft.volume=5&rft.issue=9&rft.spage=e366&rft_id=info:doi/10.1038%2Fmtna.2016.66&rft_id=info%3Apmid%2F27673562&rft.externalDocID=PMC5056990
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2162-2531&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2162-2531&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2162-2531&client=summon