Simultaneous Determination of Flavonoids in Different Parts of Citrus reticulata ‘Chachi’ Fruit by High Performance Liquid Chromatography—Photodiode Array Detection

Flavonoids are important polyphenolic secondary metabolites in plant. Citrus reticulata ‘Chachi’ fruit are rich in flavonoids and are being used as functional antioxidant ingredients for the treatment of atherosclerosis and cancer, etc. A high performance liquid chromatography-photodiode array detec...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Vol. 15; no. 8; pp. 5378 - 5388
Main Authors: Sun, Yinshi, Wang, Jianhua, Gu, Shubo, Liu, Zhengbo, Zhang, Yujie, Zhang, Xiaoxia
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 05.08.2010
MDPI
Subjects:
Agricultural production
Calibration
Chromatography
Chromatography, High Pressure Liquid - instrumentation
Chromatography, High Pressure Liquid - methods
Citrus - chemistry
Citrus Reticulata ‘Chachi’ fruit
Flavonoids
Flavonoids - analysis
Flavonoids - chemistry
high performance liquid chromatography–photodiode array detector (HPLC–PDA)
Linear Models
Quality control
quantitative analysis
Reference Standards
simultaneous determination
Solvents
Solvents - chemistry
Spectrophotometry, Ultraviolet
Time Factors
China
ISSN:1420-3049, 1420-3049
Online Access:Get full text
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Summary:Flavonoids are important polyphenolic secondary metabolites in plant. Citrus reticulata ‘Chachi’ fruit are rich in flavonoids and are being used as functional antioxidant ingredients for the treatment of atherosclerosis and cancer, etc. A high performance liquid chromatography-photodiode array detection system was used to analyze five flavonoids, namely, naringin, hesperidin, didymin, tangeretin and nobiletin, in different parts of C. reticulata ‘Chachi’ fruit. The chromatographic analysis was performed on a C18 column with a gradient elution of acetonitrile and water at a flow rate of 1.0 mL/min. Detection was carried out using a photodiode array detector at 280 nm. The calibration curves for the determination of all analytes showed good linearity over the investigated ranges (R2 > 0.9995). Precision and reproducibility were evaluated by six replicated analyses, and the R.S.D. values were less than 0.9% and 2.7%. The recoveries were between 98.37 and 103.89%. This method is promising to improve the quality control of different parts of C. reticulata ‘Chachi’ fruit.
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ISSN:1420-3049
1420-3049
DOI:10.3390/molecules15085378