Ultrastructural Imaging of Activity-Dependent Synaptic Membrane-Trafficking Events in Cultured Brain Slices

Electron microscopy can resolve synapse ultrastructure with nanometer precision, but the capture of time-resolved, activity-dependent synaptic membrane-trafficking events has remained challenging, particularly in functionally distinct synapses in a tissue context. We present a method that combines o...

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Bibliographic Details
Published in:	Neuron (Cambridge, Mass.) Vol. 108; no. 5; p. 843
Main Authors:	Imig, Cordelia, López-Murcia, Francisco José, Maus, Lydia, García-Plaza, Inés Hojas, Mortensen, Lena Sünke, Schwark, Manuela, Schwarze, Valentin, Angibaud, Julie, Nägerl, U Valentin, Taschenberger, Holger, Brose, Nils, Cooper, Benjamin H
Format:	Journal Article
Language:	English
Published:	United States 09.12.2020
Subjects:	Animals Excitatory Postsynaptic Potentials - physiology Gene Knock-In Techniques - methods Hippocampus - physiology Hippocampus - ultrastructure Mice Mice, Transgenic Microscopy, Electron - methods Microtomy - methods Organ Culture Techniques Protein Transport - physiology Synaptic Membranes - physiology Synaptic Membranes - ultrastructure High-pressure freezing active zone optogenetics Electron tomography endocytosis exocytosis synapse Electron microscopy Flash-and-freeze synaptic vesicle
ISSN:	1097-4199, 1097-4199
Online Access:	Get more information
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