Adenovirus and mycoplasma infection in an ornate box turtle ( Terrapene ornata ornata) in Hungary

A female, adult ornate box turtle ( Terrapene ornata ornata) with fatty liver was submitted for virologic examination in Hungary. Signs of an adenovirus infection including degeneration of the liver cells, enlarged nuclei and intranuclear inclusion bodies were detected by light microscopic examinati...

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Vydáno v:	Veterinary microbiology Ročník 138; číslo 1; s. 169 - 173
Hlavní autoři:	Farkas, Szilvia L., Gál, János
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Amsterdam Elsevier B.V 02.07.2009 Amsterdam; New York: Elsevier Elsevier
Témata:	Adenoviridae Adenoviridae - isolation & purification Adenoviridae Infections Adenoviridae Infections - classification Adenoviridae Infections - diagnosis Adenoviridae Infections - pathology Adenoviridae Infections - veterinary Adenovirus animal diseases animal pathology Animals Bacteriology Biological and medical sciences case studies classification diagnosis disease diagnosis DNA, Bacterial DNA, Bacterial - genetics DNA, Bacterial - isolation & purification DNA, Viral DNA, Viral - genetics DNA, Viral - isolation & purification emerging diseases fatty liver Female Fundamental and applied biological sciences. Psychology genetics hepatitis Hungary inclusion bodies Inclusion Bodies - microbiology Inclusion Bodies - pathology Inclusion Bodies, Viral Inclusion Bodies, Viral - pathology isolation & purification liver Microbiology Miscellaneous mixed infection molecular epidemiology Mycoplasma Mycoplasma - isolation & purification Mycoplasma agassizii Mycoplasma Infections Mycoplasma Infections - classification Mycoplasma Infections - diagnosis Mycoplasma Infections - pathology Mycoplasma Infections - veterinary mycoplasmosis necropsy Ornate box turtle pathology pathotypes Phylogeny Polymerase Chain Reaction sequence analysis strains Terrapene carolina carolina Terrapene ornata ornata Testudines turtles Turtles - microbiology Turtles - virology veterinary viral diseases of animals and humans Virology Hungary Europe Ornate box turtle Mycoplasma Terrapene ornata ornata Adenovirus Mollicutes Virus Mycoplasmatales Adenoviridae Microbiology Bacteria Veterinary Mycoplasmataceae
ISSN:	0378-1135, 1873-2542, 1873-2542
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Popis
Shrnutí:	A female, adult ornate box turtle ( Terrapene ornata ornata) with fatty liver was submitted for virologic examination in Hungary. Signs of an adenovirus infection including degeneration of the liver cells, enlarged nuclei and intranuclear inclusion bodies were detected by light microscopic examination. The presence of an adenovirus was later confirmed by obtaining partial sequence data from the adenoviral DNA-dependent DNA-polymerase. Phylogenetic analyses revealed that this novel chelonian adenovirus was distinct from previously described reptilian adenoviruses, not belonging to any of the recognized genera of the family Adenoviridae. As a part of the routine diagnostic procedure for chelonians the detection of herpes-, rana- and iridoviruses together with Mycoplasma spp. was attempted. Amplicons were generated by a general mycoplasma polymerase chain reaction (PCR) targeting the 16S/23S ribosomal RNA (rRNA) intergenic spacer region, as well as, a specific Mycoplasma agassizii PCR targeting the 16S rRNA gene. Based on the analyses of partial sequences of the 16S rRNA gene, the Mycoplasma sp. of the ornate box turtle seemed to be identical with the recently described eastern box turtle ( Terrapene carolina carolina) Mycoplasma sp. This is the first report of a novel chelonian adenovirus and a mycoplasma infection in an ornate box turtle ( T. ornata ornata) in Europe.
Bibliografie:	http://dx.doi.org/10.1016/j.vetmic.2009.03.016 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Case Study-2 ObjectType-Feature-4 ObjectType-Report-1 ObjectType-Article-3
ISSN:	0378-1135 1873-2542 1873-2542
DOI:	10.1016/j.vetmic.2009.03.016