A functional collagen adhesin gene, acm, in clinical isolates of Enterococcus faecium correlates with the recent success of this emerging nosocomial pathogen
Enterococcus faecium recently evolved from a generally avirulent commensal into a multidrug-resistant health care-associated pathogen causing difficult-to-treat infections, but little is known about the factors responsible for this change. We previously showed that some E. faecium strains express a...
Uloženo v:
| Vydáno v: | Infection and immunity Ročník 76; číslo 9; s. 4110 |
|---|---|
| Hlavní autoři: | , , , |
| Médium: | Journal Article |
| Jazyk: | angličtina |
| Vydáno: |
United States
01.09.2008
|
| Témata: | |
| ISSN: | 1098-5522, 1098-5522 |
| On-line přístup: | Zjistit podrobnosti o přístupu |
| Tagy: |
Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
|
| Abstract | Enterococcus faecium recently evolved from a generally avirulent commensal into a multidrug-resistant health care-associated pathogen causing difficult-to-treat infections, but little is known about the factors responsible for this change. We previously showed that some E. faecium strains express a cell wall-anchored collagen adhesin, Acm. Here we analyzed 90 E. faecium isolates (99% acm(+)) and found that the Acm protein was detected predominantly in clinically derived isolates, while the acm gene was present as a transposon-interrupted pseudogene in 12 of 47 isolates of nonclinical origin. A highly significant association between clinical (versus fecal or food) origin and collagen adherence (P <or= 0.0003) was also demonstrated, and levels of adherence were highly correlated (r = 0.879) with the amount of cell surface Acm detected by whole-cell enzyme-linked immunosorbent assay and flow cytometry. Thirty-seven of 41 sera from patients with E. faecium infections showed reactivity with recombinant Acm, while only 4 of 30 community and hospitalized patient control group sera reacted (P <or= 0.0003); importantly, antibodies to Acm were present in all 14 E. faecium endocarditis patient sera. Although pulsed-field gel electrophoresis indicated that multiple strains expressed collagen adherence, multilocus sequence typing demonstrated that the majority of collagen-adhering isolates, as well as 16 of 17 endocarditis isolates, are part of the hospital-associated E. faecium genogroup referred to as clonal complex 17 (CC17), which has emerged globally. Taken together, our findings support the hypothesis that Acm has contributed to the emergence of E. faecium and CC17 in nosocomial infections. |
|---|---|
| AbstractList | Enterococcus faecium recently evolved from a generally avirulent commensal into a multidrug-resistant health care-associated pathogen causing difficult-to-treat infections, but little is known about the factors responsible for this change. We previously showed that some E. faecium strains express a cell wall-anchored collagen adhesin, Acm. Here we analyzed 90 E. faecium isolates (99% acm(+)) and found that the Acm protein was detected predominantly in clinically derived isolates, while the acm gene was present as a transposon-interrupted pseudogene in 12 of 47 isolates of nonclinical origin. A highly significant association between clinical (versus fecal or food) origin and collagen adherence (P <or= 0.0003) was also demonstrated, and levels of adherence were highly correlated (r = 0.879) with the amount of cell surface Acm detected by whole-cell enzyme-linked immunosorbent assay and flow cytometry. Thirty-seven of 41 sera from patients with E. faecium infections showed reactivity with recombinant Acm, while only 4 of 30 community and hospitalized patient control group sera reacted (P <or= 0.0003); importantly, antibodies to Acm were present in all 14 E. faecium endocarditis patient sera. Although pulsed-field gel electrophoresis indicated that multiple strains expressed collagen adherence, multilocus sequence typing demonstrated that the majority of collagen-adhering isolates, as well as 16 of 17 endocarditis isolates, are part of the hospital-associated E. faecium genogroup referred to as clonal complex 17 (CC17), which has emerged globally. Taken together, our findings support the hypothesis that Acm has contributed to the emergence of E. faecium and CC17 in nosocomial infections. Enterococcus faecium recently evolved from a generally avirulent commensal into a multidrug-resistant health care-associated pathogen causing difficult-to-treat infections, but little is known about the factors responsible for this change. We previously showed that some E. faecium strains express a cell wall-anchored collagen adhesin, Acm. Here we analyzed 90 E. faecium isolates (99% acm(+)) and found that the Acm protein was detected predominantly in clinically derived isolates, while the acm gene was present as a transposon-interrupted pseudogene in 12 of 47 isolates of nonclinical origin. A highly significant association between clinical (versus fecal or food) origin and collagen adherence (P <or= 0.0003) was also demonstrated, and levels of adherence were highly correlated (r = 0.879) with the amount of cell surface Acm detected by whole-cell enzyme-linked immunosorbent assay and flow cytometry. Thirty-seven of 41 sera from patients with E. faecium infections showed reactivity with recombinant Acm, while only 4 of 30 community and hospitalized patient control group sera reacted (P <or= 0.0003); importantly, antibodies to Acm were present in all 14 E. faecium endocarditis patient sera. Although pulsed-field gel electrophoresis indicated that multiple strains expressed collagen adherence, multilocus sequence typing demonstrated that the majority of collagen-adhering isolates, as well as 16 of 17 endocarditis isolates, are part of the hospital-associated E. faecium genogroup referred to as clonal complex 17 (CC17), which has emerged globally. Taken together, our findings support the hypothesis that Acm has contributed to the emergence of E. faecium and CC17 in nosocomial infections.Enterococcus faecium recently evolved from a generally avirulent commensal into a multidrug-resistant health care-associated pathogen causing difficult-to-treat infections, but little is known about the factors responsible for this change. We previously showed that some E. faecium strains express a cell wall-anchored collagen adhesin, Acm. Here we analyzed 90 E. faecium isolates (99% acm(+)) and found that the Acm protein was detected predominantly in clinically derived isolates, while the acm gene was present as a transposon-interrupted pseudogene in 12 of 47 isolates of nonclinical origin. A highly significant association between clinical (versus fecal or food) origin and collagen adherence (P <or= 0.0003) was also demonstrated, and levels of adherence were highly correlated (r = 0.879) with the amount of cell surface Acm detected by whole-cell enzyme-linked immunosorbent assay and flow cytometry. Thirty-seven of 41 sera from patients with E. faecium infections showed reactivity with recombinant Acm, while only 4 of 30 community and hospitalized patient control group sera reacted (P <or= 0.0003); importantly, antibodies to Acm were present in all 14 E. faecium endocarditis patient sera. Although pulsed-field gel electrophoresis indicated that multiple strains expressed collagen adherence, multilocus sequence typing demonstrated that the majority of collagen-adhering isolates, as well as 16 of 17 endocarditis isolates, are part of the hospital-associated E. faecium genogroup referred to as clonal complex 17 (CC17), which has emerged globally. Taken together, our findings support the hypothesis that Acm has contributed to the emergence of E. faecium and CC17 in nosocomial infections. |
| Author | Murray, Barbara E Nallapareddy, Sreedhar R Singh, Kavindra V Okhuysen, Pablo C |
| Author_xml | – sequence: 1 givenname: Sreedhar R surname: Nallapareddy fullname: Nallapareddy, Sreedhar R organization: Department of Internal Medicine, Division of Infectious Diseases, Center for the Study of Emerging and Re-Emerging Pathogens, University of Texas Medical School, Houston, Texas 77030, USA – sequence: 2 givenname: Kavindra V surname: Singh fullname: Singh, Kavindra V – sequence: 3 givenname: Pablo C surname: Okhuysen fullname: Okhuysen, Pablo C – sequence: 4 givenname: Barbara E surname: Murray fullname: Murray, Barbara E |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/18591238$$D View this record in MEDLINE/PubMed |
| BookMark | eNpNkE1LxDAQhoMoft88S06etmvSNG16XMSPhQUvei7pdLKNtMnapIg_xv9qcBU8zTvDO88M7xk5dN4hIVecLTnP1e16tV4yJiqZMXVATjmrVSZlnh_-0yfkLIQ3xnhRFOqYnHAla54LdUq-VtTMDqL1Tg8U_DDoLTqqux6DdTRpXFAN44KmDgbrLCSfDX7QEQP1ht67iJMHDzAHajSCnccEmibcWz5s7GnskU4I6CINMwCGn9XY20BxxGlr3ZY6HxJmtIm_07H36fYFOTJ6CHj5W8_J68P9y91Ttnl-XN-tNhlIKWNmjNKiVCVnnap0W3KhuUDRGaylwJpBi2kCCkxV61YWUunCCJn-KLGuhM7Pyc2eu5v8-4whNqMNgCkMh34OTVkXRc6rOhmvf41zO2LX7CY76umz-Qs0_waJGnz1 |
| CitedBy_id | crossref_primary_10_1128_CMR_00058_18 crossref_primary_10_1128_AEM_03661_14 crossref_primary_10_1016_j_tim_2009_06_004 crossref_primary_10_1007_s10096_013_2029_z crossref_primary_10_1016_j_micpath_2025_107381 crossref_primary_10_1128_JCM_02526_09 crossref_primary_10_1016_j_cmi_2015_08_008 crossref_primary_10_1128_AAC_02763_16 crossref_primary_10_3390_antibiotics14090858 crossref_primary_10_1093_jac_dkaa041 crossref_primary_10_1016_j_bbadva_2025_100156 crossref_primary_10_1099_mgen_0_000938 crossref_primary_10_1111_j_1469_0691_2010_03213_x crossref_primary_10_3389_fimmu_2017_00088 crossref_primary_10_1016_j_ijfoodmicro_2011_08_014 crossref_primary_10_1089_sur_2020_188 crossref_primary_10_1186_s13028_023_00668_z crossref_primary_10_1007_s00432_015_2084_1 crossref_primary_10_2217_fmb_09_82 crossref_primary_10_1093_infdis_jiq160 crossref_primary_10_1371_journal_pone_0030319 crossref_primary_10_2217_fmb_13_66 crossref_primary_10_3389_fmicb_2019_01108 crossref_primary_10_1186_s12866_015_0468_7 crossref_primary_10_1016_j_tim_2011_11_001 crossref_primary_10_1016_j_lwt_2016_10_026 crossref_primary_10_1038_s41598_019_45441_3 crossref_primary_10_1128_spectrum_04204_22 crossref_primary_10_1038_nrmicro2761 crossref_primary_10_1016_j_biotechadv_2024_108492 crossref_primary_10_1111_j_1574_695X_2012_00997_x crossref_primary_10_1186_1471_2334_11_80 crossref_primary_10_1186_s12896_015_0151_y crossref_primary_10_1016_j_micpath_2022_105860 crossref_primary_10_1128_spectrum_01018_22 crossref_primary_10_3390_microorganisms9091900 crossref_primary_10_1016_j_micpath_2022_105745 crossref_primary_10_1097_IPC_0000000000000397 crossref_primary_10_1371_journal_pone_0103274 crossref_primary_10_3389_fmicb_2023_1254896 crossref_primary_10_1111_j_1574_6968_2009_01806_x crossref_primary_10_1099_jmm_0_016238_0 crossref_primary_10_3389_fmicb_2015_00782 crossref_primary_10_1016_j_jgar_2025_07_024 crossref_primary_10_1128_IAI_00275_09 crossref_primary_10_1128_IAI_00885_15 crossref_primary_10_1128_JCM_00319_19 crossref_primary_10_1016_j_ijantimicag_2009_10_012 crossref_primary_10_3390_antibiotics13090829 crossref_primary_10_1016_j_diagmicrobio_2012_09_006 crossref_primary_10_1016_j_micpath_2021_105380 crossref_primary_10_1016_j_idairyj_2014_06_009 crossref_primary_10_1038_s41579_024_01058_6 crossref_primary_10_1128_JCM_02346_09 crossref_primary_10_1186_s13059_019_1879_9 crossref_primary_10_1016_j_idairyj_2023_105817 crossref_primary_10_1186_1471_2180_12_135 crossref_primary_10_1016_j_idc_2016_02_006 crossref_primary_10_1111_j_1574_6976_2012_00340_x crossref_primary_10_1128_AEM_03859_13 crossref_primary_10_1016_j_micinf_2011_08_006 crossref_primary_10_1128_JCM_02283_08 crossref_primary_10_3390_antibiotics12020231 crossref_primary_10_1186_s12866_019_1539_y crossref_primary_10_1016_j_jes_2019_01_001 crossref_primary_10_1007_s10482_011_9684_9 crossref_primary_10_1111_j_1462_2920_2010_02355_x crossref_primary_10_1128_IAI_01911_14 crossref_primary_10_1128_JB_02288_14 crossref_primary_10_1111_jfpp_16423 crossref_primary_10_2903_j_efsa_2008_923 crossref_primary_10_1111_1574_6968_12418 crossref_primary_10_3390_antibiotics11070857 crossref_primary_10_1111_j_1469_0691_2010_03226_x crossref_primary_10_1128_IAI_00376_08 crossref_primary_10_2903_j_efsa_2009_1431 |
| ContentType | Journal Article |
| DBID | CGR CUY CVF ECM EIF NPM 7X8 |
| DOI | 10.1128/IAI.00375-08 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | no_fulltext_linktorsrc |
| Discipline | Medicine Biology |
| EISSN | 1098-5522 |
| ExternalDocumentID | 18591238 |
| Genre | Journal Article Research Support, N.I.H., Extramural |
| GrantInformation_xml | – fundername: NIAID NIH HHS grantid: R01 AI067861 – fundername: NIAID NIH HHS grantid: R01 AI 67861 – fundername: NCRR NIH HHS grantid: UL1 RR024148 |
| GroupedDBID | --- -DZ -~X .55 .GJ 0R~ 18M 29I 2WC 39C 3O- 4.4 41~ 53G 5GY 5RE 5VS 85S ABOCM ACGFO ADBBV AENEX AGCDD AGVNZ AI. ALMA_UNASSIGNED_HOLDINGS AOIJS BAWUL BTFSW C1A CGR CS3 CUY CVF D0S DIK DU5 E3Z EBS ECM EIF EJD F5P FRP GX1 H13 HYE HZ~ H~9 IH2 J5H KQ8 L7B MVM NEJ NPM O9- OHT OK1 P2P RHI RNS RPM RSF SJN TR2 TWZ UPT VH1 W2D W8F WH7 WHG WOQ X7M Y6R ZGI ZXP ~KM 7X8 AAGFI |
| ID | FETCH-LOGICAL-c555t-ff8a368610d87ab613a13e3dfe953e90cbe3a1c8cf79ab5458a4f35cce6e973a2 |
| IEDL.DBID | 7X8 |
| ISICitedReferencesCount | 92 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000258667500031&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 1098-5522 |
| IngestDate | Fri Sep 05 06:35:39 EDT 2025 Thu Apr 03 06:57:42 EDT 2025 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 9 |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c555t-ff8a368610d87ab613a13e3dfe953e90cbe3a1c8cf79ab5458a4f35cce6e973a2 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| OpenAccessLink | http://doi.org/10.1128/IAI.00375-08 |
| PMID | 18591238 |
| PQID | 69442179 |
| PQPubID | 23479 |
| ParticipantIDs | proquest_miscellaneous_69442179 pubmed_primary_18591238 |
| PublicationCentury | 2000 |
| PublicationDate | 2008-09-01 |
| PublicationDateYYYYMMDD | 2008-09-01 |
| PublicationDate_xml | – month: 09 year: 2008 text: 2008-09-01 day: 01 |
| PublicationDecade | 2000 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States |
| PublicationTitle | Infection and immunity |
| PublicationTitleAlternate | Infect Immun |
| PublicationYear | 2008 |
| SSID | ssj0014448 |
| Score | 2.251387 |
| Snippet | Enterococcus faecium recently evolved from a generally avirulent commensal into a multidrug-resistant health care-associated pathogen causing... |
| SourceID | proquest pubmed |
| SourceType | Aggregation Database Index Database |
| StartPage | 4110 |
| SubjectTerms | Adhesins, Bacterial - genetics Adhesins, Bacterial - metabolism Animals Antibodies, Bacterial - blood Bacterial Adhesion Bacterial Typing Techniques Cattle Chickens Cluster Analysis Collagen - metabolism Cross Infection - microbiology DNA Fingerprinting DNA Transposable Elements DNA, Bacterial - genetics Electrophoresis, Gel, Pulsed-Field Endocarditis, Bacterial - immunology Enterococcus faecium - classification Enterococcus faecium - genetics Enterococcus faecium - isolation & purification Enterococcus faecium - pathogenicity Feces - microbiology Food Microbiology Genes, Bacterial Genotype Gram-Positive Bacterial Infections - microbiology Humans Pseudogenes Sequence Analysis, DNA Swine Turkeys |
| Title | A functional collagen adhesin gene, acm, in clinical isolates of Enterococcus faecium correlates with the recent success of this emerging nosocomial pathogen |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/18591238 https://www.proquest.com/docview/69442179 |
| Volume | 76 |
| WOSCitedRecordID | wos000258667500031&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1LS8QwEB5848X3-zUHj0Z3t2mbgCCLKAq6eFDY25KmCe5hW7Wu4I_xvzqTbvEkHryUtrRNYSYzX-aRD-A44WQm-WVhUyWF9F6JrJ1FIjaJ1cZLWiKE3fXv0l5P9fv6YQrOm14YLqtsbGIw1HlpOUZ-lmgpCT7ri5dXwZxRnFudEGhMw2xEQIYLutL-Tw5BSlk3wmklYoIZTdl7R53ddm9PA_uraKnfoWVwMdfL__u5FViaQEvs1rqwClOuWIP5mmzycw0W7idp9HX46iL7szoMiEEVSI_Q5M-uGhZI5-4EjR2dIF01vZM4JDVlZIqlx1BLUJIxteMKvXF2OB7Rh95Caww9wuFdJGyJZFDJrWE1DsSM_CozICO3JTM9EhYlqUc5olmATI5c0tgb8HR99Xh5IyY8DcLGcfwuSMAmShQBsVylJiOAYDi2mnun48jpls0c3bHK-lSbjDN1RvoopnETp9PIdDZhpigLtw3ovW-TgiSZl7QubDnN-JRsiulYRiv5Dhw1AhjQPODkhilcOa4GjQh2YKuW4eCl3q5j0OYt-giZ7P757h4sNuUgrfY-zHqyAO4A5uzH-7B6OwzqRcfew_03CZvc_g |
| linkProvider | ProQuest |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=A+functional+collagen+adhesin+gene%2C+acm%2C+in+clinical+isolates+of+Enterococcus+faecium+correlates+with+the+recent+success+of+this+emerging+nosocomial+pathogen&rft.jtitle=Infection+and+immunity&rft.au=Nallapareddy%2C+Sreedhar+R&rft.au=Singh%2C+Kavindra+V&rft.au=Okhuysen%2C+Pablo+C&rft.au=Murray%2C+Barbara+E&rft.date=2008-09-01&rft.issn=1098-5522&rft.eissn=1098-5522&rft.volume=76&rft.issue=9&rft.spage=4110&rft_id=info:doi/10.1128%2FIAI.00375-08&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1098-5522&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1098-5522&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1098-5522&client=summon |