Ribosomal protein L5 and transcription factor IIIA from Arabidopsis thaliana bind in vitro specifically Potato spindle tuber viroid RNA

Potato spindle tuber viroid (PSTVd) contains an element of tertiary structure -loop E- also present in eukaryotic 5S rRNA. The ribosomal protein L5 and transcription factor IIIA (TFIIIA) from Arabidopsis thaliana bind 5S rRNA in vitro and in vivo, mediating different functions that include nucleocyt...

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Vydané v:	Archives of virology Ročník 156; číslo 3; s. 529 - 533
Hlavní autori:	Eiras, Marcelo, Nohales, María Angeles, Kitajima, Elliot W, Flores, Ricardo, Daròs, José Antonio
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	Vienna Vienna : Springer Vienna 01.03.2011 Springer Vienna Springer Springer Nature B.V
Predmet:	Arabidopsis Arabidopsis - virology Arabidopsis thaliana Biological and medical sciences Biomedical and Life Sciences Biomedicine Brief Report Fundamental and applied biological sciences. Psychology genetics Infectious Diseases Medical Microbiology metabolism Microbiology Miscellaneous nucleocytoplasmic transport Phytopathology. Animal pests. Plant and forest protection Plant Proteins Plant Proteins - metabolism Plant viruses and viroids Potato spindle tuber viroid Protein Binding Proteins ribosomal proteins Ribosomal Proteins - metabolism ribosomal RNA RNA, Ribosomal, 5S RNA, Ribosomal, 5S - metabolism RNA, Viral RNA, Viral - metabolism Solanum tuberosum Solanum tuberosum - virology transcription (genetics) Transcription Factor TFIIIA Transcription Factor TFIIIA - metabolism Transcription factors transcriptional activation Viroids Viroids - genetics Virology Yeast Brazil Potato Spindle Tuber Viroid Label Transfer Central Conserve Region Electrophoretic Mobility Shift Assay Infectious Cycle Pospiviroid Plant pathogen Pospiviroidae In vitro Ribosomal protein Virus Arabidopsis thaliana Cruciferae Dicotyledones Viroid Angiospermae Spermatophyta Experimental plant Transcription factor Potato spindle tuber viroid
ISSN:	0304-8608, 1432-8798, 1432-8798
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Shrnutí:	Potato spindle tuber viroid (PSTVd) contains an element of tertiary structure -loop E- also present in eukaryotic 5S rRNA. The ribosomal protein L5 and transcription factor IIIA (TFIIIA) from Arabidopsis thaliana bind 5S rRNA in vitro and in vivo, mediating different functions that include nucleocytoplasmic transport and transcription activation, respectively. We show that A. thaliana L5 and TFIIIA also bind PSTVd (+) RNA in vitro with the same affinity as they bind 5S rRNA, whereas the affinity for a chloroplastic viroid is significantly lower. These two proteins might participate in the synthesis and delivery of PSTVd RNA in vivo.
Bibliografia:	http://dx.doi.org/10.1007/s00705-010-0867-x ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 14 ObjectType-Article-1 ObjectType-Feature-2 content type line 23
ISSN:	0304-8608 1432-8798 1432-8798
DOI:	10.1007/s00705-010-0867-x