Generation of Blastocyst-like Structures from Mouse Embryonic and Adult Cell Cultures

A single mouse blastomere from an embryo until the 8-cell stage can generate an entire blastocyst. Whether laboratory-cultured cells retain a similar generative capacity remains unknown. Starting from a single stem cell type, extended pluripotent stem (EPS) cells, we established a 3D differentiation...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	Cell Ročník 179; číslo 3; s. 687
Hlavní autoři:	Li, Ronghui, Zhong, Cuiqing, Yu, Yang, Liu, Haisong, Sakurai, Masahiro, Yu, Leqian, Min, Zheying, Shi, Lei, Wei, Yulei, Takahashi, Yuta, Liao, Hsin-Kai, Qiao, Jie, Deng, Hongkui, Nuñez-Delicado, Estrella, Rodriguez Esteban, Concepcion, Wu, Jun, Izpisua Belmonte, Juan Carlos
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States 17.10.2019
Témata:	Animals Blastocyst - cytology Blastocyst - metabolism Cell Differentiation Cell Line Cell Lineage Cells, Cultured Cellular Reprogramming Techniques - methods Embryo Implantation Female Humans Induced Pluripotent Stem Cells - cytology Induced Pluripotent Stem Cells - metabolism Male Mice Mice, Inbred C57BL Mice, Inbred ICR Mouse Embryonic Stem Cells - cytology Mouse Embryonic Stem Cells - metabolism Research Embryo Creation - methods Transcriptome blastoids reprogramming extended pluripotent stem cells EPS-blastoid implantation blastocyst EPS cells totipotent
ISSN:	1097-4172, 1097-4172
On-line přístup:	Zjistit podrobnosti o přístupu
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Abstract	A single mouse blastomere from an embryo until the 8-cell stage can generate an entire blastocyst. Whether laboratory-cultured cells retain a similar generative capacity remains unknown. Starting from a single stem cell type, extended pluripotent stem (EPS) cells, we established a 3D differentiation system that enabled the generation of blastocyst-like structures (EPS-blastoids) through lineage segregation and self-organization. EPS-blastoids resembled blastocysts in morphology and cell-lineage allocation and recapitulated key morphogenetic events during preimplantation and early postimplantation development in vitro. Upon transfer, some EPS-blastoids underwent implantation, induced decidualization, and generated live, albeit disorganized, tissues in utero. Single-cell and bulk RNA-sequencing analysis revealed that EPS-blastoids contained all three blastocyst cell lineages and shared transcriptional similarity with natural blastocysts. We also provide proof of concept that EPS-blastoids can be generated from adult cells via cellular reprogramming. EPS-blastoids provide a unique platform for studying early embryogenesis and pave the way to creating viable synthetic embryos by using cultured cells.
AbstractList	A single mouse blastomere from an embryo until the 8-cell stage can generate an entire blastocyst. Whether laboratory-cultured cells retain a similar generative capacity remains unknown. Starting from a single stem cell type, extended pluripotent stem (EPS) cells, we established a 3D differentiation system that enabled the generation of blastocyst-like structures (EPS-blastoids) through lineage segregation and self-organization. EPS-blastoids resembled blastocysts in morphology and cell-lineage allocation and recapitulated key morphogenetic events during preimplantation and early postimplantation development in vitro. Upon transfer, some EPS-blastoids underwent implantation, induced decidualization, and generated live, albeit disorganized, tissues in utero. Single-cell and bulk RNA-sequencing analysis revealed that EPS-blastoids contained all three blastocyst cell lineages and shared transcriptional similarity with natural blastocysts. We also provide proof of concept that EPS-blastoids can be generated from adult cells via cellular reprogramming. EPS-blastoids provide a unique platform for studying early embryogenesis and pave the way to creating viable synthetic embryos by using cultured cells.A single mouse blastomere from an embryo until the 8-cell stage can generate an entire blastocyst. Whether laboratory-cultured cells retain a similar generative capacity remains unknown. Starting from a single stem cell type, extended pluripotent stem (EPS) cells, we established a 3D differentiation system that enabled the generation of blastocyst-like structures (EPS-blastoids) through lineage segregation and self-organization. EPS-blastoids resembled blastocysts in morphology and cell-lineage allocation and recapitulated key morphogenetic events during preimplantation and early postimplantation development in vitro. Upon transfer, some EPS-blastoids underwent implantation, induced decidualization, and generated live, albeit disorganized, tissues in utero. Single-cell and bulk RNA-sequencing analysis revealed that EPS-blastoids contained all three blastocyst cell lineages and shared transcriptional similarity with natural blastocysts. We also provide proof of concept that EPS-blastoids can be generated from adult cells via cellular reprogramming. EPS-blastoids provide a unique platform for studying early embryogenesis and pave the way to creating viable synthetic embryos by using cultured cells. A single mouse blastomere from an embryo until the 8-cell stage can generate an entire blastocyst. Whether laboratory-cultured cells retain a similar generative capacity remains unknown. Starting from a single stem cell type, extended pluripotent stem (EPS) cells, we established a 3D differentiation system that enabled the generation of blastocyst-like structures (EPS-blastoids) through lineage segregation and self-organization. EPS-blastoids resembled blastocysts in morphology and cell-lineage allocation and recapitulated key morphogenetic events during preimplantation and early postimplantation development in vitro. Upon transfer, some EPS-blastoids underwent implantation, induced decidualization, and generated live, albeit disorganized, tissues in utero. Single-cell and bulk RNA-sequencing analysis revealed that EPS-blastoids contained all three blastocyst cell lineages and shared transcriptional similarity with natural blastocysts. We also provide proof of concept that EPS-blastoids can be generated from adult cells via cellular reprogramming. EPS-blastoids provide a unique platform for studying early embryogenesis and pave the way to creating viable synthetic embryos by using cultured cells.
Author	Li, Ronghui Rodriguez Esteban, Concepcion Zhong, Cuiqing Izpisua Belmonte, Juan Carlos Qiao, Jie Yu, Leqian Liu, Haisong Wu, Jun Yu, Yang Takahashi, Yuta Deng, Hongkui Liao, Hsin-Kai Shi, Lei Min, Zheying Sakurai, Masahiro Wei, Yulei Nuñez-Delicado, Estrella
Author_xml	– sequence: 1 givenname: Ronghui surname: Li fullname: Li, Ronghui organization: Gene Expression Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA – sequence: 2 givenname: Cuiqing surname: Zhong fullname: Zhong, Cuiqing organization: Gene Expression Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA – sequence: 3 givenname: Yang surname: Yu fullname: Yu, Yang organization: Gene Expression Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology and Key Laboratory of Assisted Reproduction, Ministry of Education, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China; Stem Cell Research Center, Peking University Third Hospital, Beijing, 100191, China – sequence: 4 givenname: Haisong surname: Liu fullname: Liu, Haisong organization: Gene Expression Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA – sequence: 5 givenname: Masahiro surname: Sakurai fullname: Sakurai, Masahiro organization: Gene Expression Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA; Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA – sequence: 6 givenname: Leqian surname: Yu fullname: Yu, Leqian organization: Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA – sequence: 7 givenname: Zheying surname: Min fullname: Min, Zheying organization: Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology and Key Laboratory of Assisted Reproduction, Ministry of Education, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China – sequence: 8 givenname: Lei surname: Shi fullname: Shi, Lei organization: Gene Expression Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA; Universidad Catolica, San Antonio de Murcia, Campus de los Jeronimos, 135, Guadalupe 30107, Murcia, Spain – sequence: 9 givenname: Yulei surname: Wei fullname: Wei, Yulei organization: Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, 529020, China – sequence: 10 givenname: Yuta surname: Takahashi fullname: Takahashi, Yuta organization: Gene Expression Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA – sequence: 11 givenname: Hsin-Kai surname: Liao fullname: Liao, Hsin-Kai organization: Gene Expression Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA; Universidad Catolica, San Antonio de Murcia, Campus de los Jeronimos, 135, Guadalupe 30107, Murcia, Spain – sequence: 12 givenname: Jie surname: Qiao fullname: Qiao, Jie organization: Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology and Key Laboratory of Assisted Reproduction, Ministry of Education, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China – sequence: 13 givenname: Hongkui surname: Deng fullname: Deng, Hongkui organization: University of Peking, 5 Yiheyuan Rd, Haidian Qu, Beijing 100871, China – sequence: 14 givenname: Estrella surname: Nuñez-Delicado fullname: Nuñez-Delicado, Estrella organization: Universidad Catolica, San Antonio de Murcia, Campus de los Jeronimos, 135, Guadalupe 30107, Murcia, Spain – sequence: 15 givenname: Concepcion surname: Rodriguez Esteban fullname: Rodriguez Esteban, Concepcion organization: Gene Expression Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA – sequence: 16 givenname: Jun surname: Wu fullname: Wu, Jun email: jun2.wu@utsouthwestern.edu organization: Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. Electronic address: jun2.wu@utsouthwestern.edu – sequence: 17 givenname: Juan Carlos surname: Izpisua Belmonte fullname: Izpisua Belmonte, Juan Carlos email: belmonte@salk.edu organization: Gene Expression Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA. Electronic address: belmonte@salk.edu
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/31626770$$D View this record in MEDLINE/PubMed
BookMark	eNpNkE1LxDAYhIOsuB_6BzxIjl5a89m0x7Wsq7DiwfVc0uQtdG2bNUkP--_tooIwMHN4GIZZotngBkDolpKUEpo9HFIDXZcyQouUTGLFBVpQUqhEUMVm__IcLUM4EEJyKeUVmnOasUwpskAfWxjA69i6AbsGP3Y6RGdOISZd-wn4PfrRxNFDwI13PX51YwC86Wt_ckNrsB4sXtuxi7icpuBySmf4Gl02ugtw8-srtH_a7MvnZPe2fSnXu8RIKWLScGmFEHVutJTECmkaSmqmC84tGNaIjDENpra5qrOcKcEp1CAtN5SYxrIVuv-pPXr3NUKIVd-G8yd6gGloxThRlBNK1ITe_aJj3YOtjr7ttT9Vf0-wbwb4Y98
CitedBy_id	crossref_primary_10_1016_j_ceb_2020_05_010 crossref_primary_10_1016_j_stem_2022_05_001 crossref_primary_10_3390_biology13020071 crossref_primary_10_1530_REP_21_0478 crossref_primary_10_1016_j_gde_2022_101970 crossref_primary_10_1016_j_copbio_2020_06_005 crossref_primary_10_3390_biomedicines12122714 crossref_primary_10_1073_pnas_2018505118 crossref_primary_10_1016_j_semcdb_2022_05_023 crossref_primary_10_1016_j_tcb_2021_07_008 crossref_primary_10_1016_j_ydbio_2021_03_025 crossref_primary_10_1097_RD9_0000000000000042 crossref_primary_10_3389_fcell_2024_1386739 crossref_primary_10_1016_j_ecoenv_2023_114608 crossref_primary_10_1093_molehr_gaac008 crossref_primary_10_1093_humupd_dmae033 crossref_primary_10_3389_fcell_2021_801468 crossref_primary_10_3389_fcell_2021_655993 crossref_primary_10_1096_fj_202301356R crossref_primary_10_3390_cells13070628 crossref_primary_10_1007_s10815_023_02773_4 crossref_primary_10_1007_s43032_025_01939_y crossref_primary_10_1016_j_bioactmat_2023_05_007 crossref_primary_10_3390_ijms25116045 crossref_primary_10_1016_j_celrep_2023_112379 crossref_primary_10_1002_jcp_31222 crossref_primary_10_1016_j_gde_2022_101985 crossref_primary_10_1002_bies_202200029 crossref_primary_10_1016_j_gde_2023_102135 crossref_primary_10_1016_j_theriogenology_2021_12_021 crossref_primary_10_1002_advs_202408852 crossref_primary_10_1007_s11427_024_2741_1 crossref_primary_10_1038_s41556_023_01214_9 crossref_primary_10_3390_cells10061461 crossref_primary_10_1093_humupd_dmaf006 crossref_primary_10_1002_adhm_202301067 crossref_primary_10_1016_j_medntd_2025_100393 crossref_primary_10_1038_s41586_021_03372_y crossref_primary_10_1038_s42003_023_05283_2 crossref_primary_10_1016_j_gde_2025_102377 crossref_primary_10_1016_j_gde_2023_102134 crossref_primary_10_1038_s41421_021_00316_8 crossref_primary_10_1126_science_aaz6976 crossref_primary_10_1016_j_gde_2020_06_008 crossref_primary_10_1007_s10815_019_01655_y crossref_primary_10_3389_fcell_2022_838356 crossref_primary_10_1016_j_devcel_2024_03_029 crossref_primary_10_1038_s41422_022_00668_0 crossref_primary_10_1111_cpr_12754 crossref_primary_10_1242_jcs_255018 crossref_primary_10_1016_j_jgg_2020_02_003 crossref_primary_10_1021_acs_est_5c06532 crossref_primary_10_1016_j_tig_2022_03_012 crossref_primary_10_1080_19378629_2022_2126783 crossref_primary_10_3390_cells11213516 crossref_primary_10_1016_j_ecoenv_2022_113745 crossref_primary_10_3389_fimmu_2021_750808 crossref_primary_10_1016_j_stem_2022_01_010 crossref_primary_10_1002_advs_202304987 crossref_primary_10_1016_j_cdev_2024_203975 crossref_primary_10_1038_s41421_025_00780_6 crossref_primary_10_1016_j_stem_2023_03_009 crossref_primary_10_7554_eLife_52504 crossref_primary_10_1111_cpr_13317 crossref_primary_10_1038_s41563_020_00829_9 crossref_primary_10_3390_jdb12020014 crossref_primary_10_1111_dgd_12713 crossref_primary_10_1038_s41467_021_23320_8 crossref_primary_10_1016_j_devcel_2024_05_009 crossref_primary_10_3390_genes11090976 crossref_primary_10_1093_humrep_deae044 crossref_primary_10_1007_s00018_022_04589_4 crossref_primary_10_1186_s13287_023_03583_2 crossref_primary_10_1016_j_tibtech_2021_03_010 crossref_primary_10_1242_dev_201015 crossref_primary_10_1002_advs_202415956 crossref_primary_10_1111_cpr_13396 crossref_primary_10_3389_fcell_2024_1482054 crossref_primary_10_1038_s41592_019_0674_6 crossref_primary_10_1016_j_cell_2024_05_051 crossref_primary_10_1016_j_bbrc_2021_08_047 crossref_primary_10_26508_lsa_202201608 crossref_primary_10_1016_j_gde_2023_102069 crossref_primary_10_1093_humupd_dmaa027 crossref_primary_10_3390_ijms23042067 crossref_primary_10_1016_j_stemcr_2025_102514 crossref_primary_10_1016_j_cell_2023_07_018 crossref_primary_10_1242_dev_201187 crossref_primary_10_3389_fcell_2022_1055139 crossref_primary_10_1038_s41596_024_00984_2 crossref_primary_10_1186_s13578_025_01410_0 crossref_primary_10_1016_j_cell_2025_07_018 crossref_primary_10_1016_j_gde_2025_102342 crossref_primary_10_1016_j_stem_2022_04_003 crossref_primary_10_1016_j_stem_2023_04_003 crossref_primary_10_1016_j_gde_2020_02_013 crossref_primary_10_1089_scd_2022_0061 crossref_primary_10_1242_dev_200812 crossref_primary_10_1016_j_stem_2024_12_006 crossref_primary_10_1093_nar_gkac230 crossref_primary_10_1016_j_ydbio_2020_12_010 crossref_primary_10_1242_dev_203090 crossref_primary_10_3390_cells11121923 crossref_primary_10_1007_s00109_021_02053_7 crossref_primary_10_1186_s13287_025_04507_y crossref_primary_10_1016_j_cbi_2023_110806 crossref_primary_10_1016_j_semcdb_2022_07_002 crossref_primary_10_1590_1984_3143_ar2025_0056 crossref_primary_10_1007_s12015_023_10527_z crossref_primary_10_3389_fbioe_2020_00781 crossref_primary_10_1038_s41421_024_00693_w crossref_primary_10_1093_molehr_gaaa005 crossref_primary_10_1016_j_jia_2024_02_007 crossref_primary_10_1038_s41467_021_25853_4 crossref_primary_10_1186_s13287_023_03290_y crossref_primary_10_1186_s13619_023_00176_5 crossref_primary_10_1016_j_devcel_2021_06_002 crossref_primary_10_1038_s41422_025_01172_x crossref_primary_10_1186_s13059_025_03577_8 crossref_primary_10_1007_s11427_024_2774_2 crossref_primary_10_1016_j_cell_2022_01_005 crossref_primary_10_1016_j_ydbio_2020_04_003 crossref_primary_10_1093_biolre_ioac135 crossref_primary_10_1016_j_cobme_2020_02_003 crossref_primary_10_1016_j_cell_2024_05_027 crossref_primary_10_1016_j_chemosphere_2020_127899 crossref_primary_10_1038_s41556_023_01282_x crossref_primary_10_1111_cpr_13128 crossref_primary_10_1002_adma_201907966 crossref_primary_10_1016_j_reprotox_2020_07_014 crossref_primary_10_1039_D1BM00718A crossref_primary_10_1038_s41586_021_03356_y crossref_primary_10_1016_j_devcel_2021_12_022 crossref_primary_10_1038_s41422_022_00686_y crossref_primary_10_1007_s11427_022_2209_3 crossref_primary_10_1093_molehr_gaae009 crossref_primary_10_1093_humupd_dmaa054 crossref_primary_10_1038_s12276_023_01097_8 crossref_primary_10_1016_j_gde_2023_102088 crossref_primary_10_1038_s41556_023_01341_3 crossref_primary_10_1126_sciadv_ado1350 crossref_primary_10_1186_s13287_025_04392_5 crossref_primary_10_3390_ijms21114115 crossref_primary_10_1016_j_devcel_2025_08_004 crossref_primary_10_1016_j_scr_2022_102857 crossref_primary_10_1007_s11427_023_2557_x crossref_primary_10_1016_j_stem_2023_08_002 crossref_primary_10_1038_s41596_020_00417_w crossref_primary_10_1093_biolre_ioac096 crossref_primary_10_1016_j_isci_2025_112659 crossref_primary_10_1093_procel_pwad049 crossref_primary_10_1016_j_tig_2023_10_010 crossref_primary_10_1186_s12958_022_00973_8 crossref_primary_10_3390_ijms241713655 crossref_primary_10_1038_d41586_021_00581_3 crossref_primary_10_1155_2021_8817581 crossref_primary_10_1007_s43032_021_00501_w crossref_primary_10_1016_j_tibtech_2025_02_015 crossref_primary_10_4252_wjsc_v15_i7_734 crossref_primary_10_1007_s43032_021_00523_4 crossref_primary_10_3389_fcell_2022_840492 crossref_primary_10_3390_ijms22095011 crossref_primary_10_1002_rmb2_12522 crossref_primary_10_4142_jvs_24004 crossref_primary_10_1016_j_cell_2020_11_003 crossref_primary_10_1016_j_stem_2023_04_011 crossref_primary_10_1016_j_devcel_2023_09_013 crossref_primary_10_1242_dev_203116 crossref_primary_10_1242_dev_189845 crossref_primary_10_1002_advs_202004250 crossref_primary_10_1016_j_stem_2024_01_009 crossref_primary_10_1134_S1062360420050045
ContentType	Journal Article
Copyright	Copyright © 2019 Elsevier Inc. All rights reserved.
Copyright_xml	– notice: Copyright © 2019 Elsevier Inc. All rights reserved.
DBID	CGR CUY CVF ECM EIF NPM 7X8
DOI	10.1016/j.cell.2019.09.029
DatabaseName	Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic
DatabaseTitle	MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic
DatabaseTitleList	MEDLINE - Academic MEDLINE
Database_xml	– sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database
DeliveryMethod	no_fulltext_linktorsrc
Discipline	Biology
EISSN	1097-4172
ExternalDocumentID	31626770
Genre	Journal Article
GroupedDBID	--- --K -DZ -ET -~X 0R~ 0SF 0WA 1RT 1~5 29B 2FS 2WC 3EH 4.4 457 4G. 53G 5GY 5RE 62- 6I. 6J9 7-5 85S AACTN AAEDT AAEDW AAFTH AAFWJ AAHBH AAKRW AAKUH AALRI AAMRU AAVLU AAXUO ABCQX ABJNI ABMAC ABOCM ABVKL ACGFO ACGFS ACNCT ADBBV ADEZE ADVLN AEFWE AENEX AEXQZ AFTJW AGHSJ AGKMS AHHHB AITUG AKAPO AKRWK ALMA_UNASSIGNED_HOLDINGS AMRAJ ASPBG AVWKF AZFZN BAWUL CGR CS3 CUY CVF DIK DU5 E3Z EBS ECM EIF EJD F5P FCP FDB FIRID HH5 IH2 IHE IXB J1W JIG K-O KOO KQ8 L7B LX5 M3Z M41 N9A NPM O-L O9- OK1 P2P RCE RIG RNS ROL RPZ SCP SDG SDP SES SSZ TAE TN5 TR2 TWZ UKR UPT VQA WH7 YZZ ZCA 7X8 AAYWO ABDGV ACVFH ADCNI ADXHL AEUPX AFPUW AIGII AKBMS AKYEP APXCP EFKBS
ID	FETCH-LOGICAL-c554t-f35d444b8ca550d45cf10b2a933dec2f4622aecbd87b6827431ebe5d3c10cfd2
IEDL.DBID	7X8
ISICitedReferencesCount	207
ISICitedReferencesURI	http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000490744100012&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN	1097-4172
IngestDate	Sun Sep 28 07:05:08 EDT 2025 Wed Feb 19 02:30:49 EST 2025
IsDoiOpenAccess	false
IsOpenAccess	true
IsPeerReviewed	true
IsScholarly	true
Issue	3
Keywords	blastoids reprogramming extended pluripotent stem cells EPS-blastoid implantation blastocyst EPS cells totipotent
Language	English
License	Copyright © 2019 Elsevier Inc. All rights reserved.
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c554t-f35d444b8ca550d45cf10b2a933dec2f4622aecbd87b6827431ebe5d3c10cfd2
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
OpenAccessLink	http://www.cell.com/article/S0092867419310803/pdf
PMID	31626770
PQID	2307130107
PQPubID	23479
ParticipantIDs	proquest_miscellaneous_2307130107 pubmed_primary_31626770
PublicationCentury	2000
PublicationDate	2019-10-17
PublicationDateYYYYMMDD	2019-10-17
PublicationDate_xml	– month: 10 year: 2019 text: 2019-10-17 day: 17
PublicationDecade	2010
PublicationPlace	United States
PublicationPlace_xml	– name: United States
PublicationTitle	Cell
PublicationTitleAlternate	Cell
PublicationYear	2019
References	31780830 - Nat Methods. 2019 Dec;16(12):1207
References_xml	– reference: 31780830 - Nat Methods. 2019 Dec;16(12):1207
SSID	ssj0008555
Score	2.661703
Snippet	A single mouse blastomere from an embryo until the 8-cell stage can generate an entire blastocyst. Whether laboratory-cultured cells retain a similar...
SourceID	proquest pubmed
SourceType	Aggregation Database Index Database
StartPage	687
SubjectTerms	Animals Blastocyst - cytology Blastocyst - metabolism Cell Differentiation Cell Line Cell Lineage Cells, Cultured Cellular Reprogramming Techniques - methods Embryo Implantation Female Humans Induced Pluripotent Stem Cells - cytology Induced Pluripotent Stem Cells - metabolism Male Mice Mice, Inbred C57BL Mice, Inbred ICR Mouse Embryonic Stem Cells - cytology Mouse Embryonic Stem Cells - metabolism Research Embryo Creation - methods Transcriptome
Title	Generation of Blastocyst-like Structures from Mouse Embryonic and Adult Cell Cultures
URI	https://www.ncbi.nlm.nih.gov/pubmed/31626770 https://www.proquest.com/docview/2307130107
Volume	179
WOSCitedRecordID	wos000490744100012&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText
inHoldings	1
isFullTextHit
isPrint
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1LS8NAEF7UKnjx_agvVvC6mGQ32c1Jamnx0lKwQm9ln1DUpDap0H_vbJLSmwheckqWzbxndvYbhB5MasAPg34bLixhzjCSauZIYKVSRoN6KVcNm-DDoZhM0lFTcCuatsq1TawMtcm1r5E_-oZlsLeQrTzNv4ifGuVPV5sRGtuoRSGU8VLNJxu0cBFXU0_9ISth4KmbSzN1f5cvjPvWrrTCOf0txKxcTf_wv5s8QgdNkIk7tVQcoy2bnaC9euzk6hS91VjTniU4d_gZAugy16uiJB-zd4tfK0jZJeTh2F8-wYN8WVjc-1SLlcfRxTIzuONhO3AXfg7XoJy2OEPjfm_cfSHNeAWiIYYoiaOxYYwpoSWkKYbF2oWBimRKqbE6ciyJImm1MoKrREQ-1ACOx4bqMNDOROdoJ8sze4mwcyk1gZAJDQ28FUlpA0U5rKeY5SJpo_s1uaYgvZ7yMrOw-emGYG10UdN8Oq9hNqY0hGSL8-DqD19fo33PSu9UQn6DWg50196iXf1dzorFXSUW8ByOBj8ncsNr
linkProvider	ProQuest
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Generation+of+Blastocyst-like+Structures+from+Mouse+Embryonic+and+Adult+Cell+Cultures&rft.jtitle=Cell&rft.au=Li%2C+Ronghui&rft.au=Zhong%2C+Cuiqing&rft.au=Yu%2C+Yang&rft.au=Liu%2C+Haisong&rft.date=2019-10-17&rft.issn=1097-4172&rft.eissn=1097-4172&rft.volume=179&rft.issue=3&rft.spage=687&rft_id=info:doi/10.1016%2Fj.cell.2019.09.029&rft.externalDBID=NO_FULL_TEXT
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1097-4172&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1097-4172&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1097-4172&client=summon