Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein

Mitochondrial ribosomes translate membrane integral core subunits of the oxidative phosphorylation system encoded by mtDNA. These translation products associate with nuclear-encoded, imported proteins to form enzyme complexes that produce ATP. Here, we show that human mitochondrial ribosomes display...

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Vydáno v:	Cell Ročník 167; číslo 2; s. 471
Hlavní autoři:	Richter-Dennerlein, Ricarda, Oeljeklaus, Silke, Lorenzi, Isotta, Ronsör, Christin, Bareth, Bettina, Schendzielorz, Alexander Benjamin, Wang, Cong, Warscheid, Bettina, Rehling, Peter, Dennerlein, Sven
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States 06.10.2016
Témata:	Active Transport, Cell Nucleus Cell Line, Tumor Cyclooxygenase 1 - biosynthesis Cyclooxygenase 1 - genetics Cyclooxygenase 1 - metabolism DNA, Mitochondrial - genetics Electron Transport Complex IV - biosynthesis Electron Transport Complex IV - genetics Electron Transport Complex IV - metabolism HEK293 Cells Humans Membrane Proteins - biosynthesis Membrane Proteins - genetics Membrane Proteins - metabolism Mitochondria - enzymology Mitochondrial Proteins - biosynthesis Mitochondrial Proteins - genetics Mitochondrial Proteins - metabolism Oxidative Phosphorylation Ribosomes - metabolism RNA, Messenger - biosynthesis RNA, Messenger - genetics RNA, Mitochondrial mitochondrial translation assembly OXPHOS C12ORF62 COX1 cytochrome c oxidase translation regulation mitochondrial ribosome MITRAC translational plasticity
ISSN:	1097-4172, 1097-4172
On-line přístup:	Zjistit podrobnosti o přístupu
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Shrnutí:	Mitochondrial ribosomes translate membrane integral core subunits of the oxidative phosphorylation system encoded by mtDNA. These translation products associate with nuclear-encoded, imported proteins to form enzyme complexes that produce ATP. Here, we show that human mitochondrial ribosomes display translational plasticity to cope with the supply of imported nuclear-encoded subunits. Ribosomes expressing mitochondrial-encoded COX1 mRNA selectively engage with cytochrome c oxidase assembly factors in the inner membrane. Assembly defects of the cytochrome c oxidase arrest mitochondrial translation in a ribosome nascent chain complex with a partially membrane-inserted COX1 translation product. This complex represents a primed state of the translation product that can be retrieved for assembly. These findings establish a mammalian translational plasticity pathway in mitochondria that enables adaptation of mitochondrial protein synthesis to the influx of nuclear-encoded subunits.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1097-4172 1097-4172
DOI:	10.1016/j.cell.2016.09.003