Small RNA Sequencing across Diverse Biofluids Identifies Optimal Methods for exRNA Isolation

Poor reproducibility within and across studies arising from lack of knowledge regarding the performance of extracellular RNA (exRNA) isolation methods has hindered progress in the exRNA field. A systematic comparison of 10 exRNA isolation methods across 5 biofluids revealed marked differences in the...

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Vydáno v:	Cell Ročník 177; číslo 2; s. 446
Hlavní autoři:	Srinivasan, Srimeenakshi, Yeri, Ashish, Cheah, Pike See, Chung, Allen, Danielson, Kirsty, De Hoff, Peter, Filant, Justyna, Laurent, Clara D, Laurent, Lucie D, Magee, Rogan, Moeller, Courtney, Murthy, Venkatesh L, Nejad, Parham, Paul, Anu, Rigoutsos, Isidore, Rodosthenous, Rodosthenis, Shah, Ravi V, Simonson, Bridget, To, Cuong, Wong, David, Yan, Irene K, Zhang, Xuan, Balaj, Leonora, Breakefield, Xandra O, Daaboul, George, Gandhi, Roopali, Lapidus, Jodi, Londin, Eric, Patel, Tushar, Raffai, Robert L, Sood, Anil K, Alexander, Roger P, Das, Saumya, Laurent, Louise C
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States 04.04.2019
Témata:	Adult Body Fluids - chemistry Cell Line Cell-Free Nucleic Acids - isolation & purification Circulating MicroRNA - isolation & purification Extracellular Vesicles - metabolism Female Healthy Volunteers Humans Male MicroRNAs - isolation & purification MicroRNAs - metabolism Reproducibility of Results RNA - isolation & purification RNA - metabolism Sequence Analysis, RNA - methods extracellular vesicles lipoprotein ribonucleoprotein extracellular RNA
ISSN:	1097-4172, 1097-4172
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