High-Throughput Mapping of B Cell Receptor Sequences to Antigen Specificity

B cell receptor (BCR) sequencing is a powerful tool for interrogating immune responses to infection and vaccination, but it provides limited information about the antigen specificity of the sequenced BCRs. Here, we present LIBRA-seq (linking B cell receptor to antigen specificity through sequencing)...

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Bibliographic Details
Published in:	Cell Vol. 179; no. 7; p. 1636
Main Authors:	Setliff, Ian, Shiakolas, Andrea R, Pilewski, Kelsey A, Murji, Amyn A, Mapengo, Rutendo E, Janowska, Katarzyna, Richardson, Simone, Oosthuysen, Charissa, Raju, Nagarajan, Ronsard, Larance, Kanekiyo, Masaru, Qin, Juliana S, Kramer, Kevin J, Greenplate, Allison R, McDonnell, Wyatt J, Graham, Barney S, Connors, Mark, Lingwood, Daniel, Acharya, Priyamvada, Morris, Lynn, Georgiev, Ivelin S
Format:	Journal Article
Language:	English
Published:	United States 12.12.2019
Subjects:	Antibodies, Neutralizing - chemistry Antibodies, Neutralizing - immunology Antigens - chemistry Antigens - immunology Cells, Cultured Epitope Mapping - methods Epitopes - chemistry Epitopes - immunology HEK293 Cells High-Throughput Nucleotide Sequencing - methods High-Throughput Screening Assays - methods HIV Antibodies - chemistry HIV Antibodies - immunology Humans Receptors, Antigen, B-Cell - chemistry Receptors, Antigen, B-Cell - immunology Sequence Analysis, DNA - methods Single-Cell Analysis - methods THP-1 Cells influenza broadly neutralizing antibody HIV single cell immunology antibody discovery antibodies B cells systems immunology antibody repertoire
ISSN:	1097-4172, 1097-4172
Online Access:	Get more information
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