Poly(l-Lactic Acid)/Gelatin Fibrous Scaffold Loaded with Simvastatin/Beta-Cyclodextrin-Modified Hydroxyapatite Inclusion Complex for Bone Tissue Regeneration

Recently, the application of nanostructured materials in the field of tissue engineering has garnered attention to mediate treatment and regeneration of bone defects. In this study, poly(l‐lactic acid) (PLLA)/gelatin (PG) fibrous scaffolds are fabricated and β‐cyclodextrin (βCD) grafted nano‐hydroxy...

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Veröffentlicht in:Macromolecular bioscience Jg. 16; H. 7; S. 1027 - 1038
Hauptverfasser: Lee, Jung Bok, Kim, Ji Eun, Balikov, Daniel A., Bae, Min Soo, Heo, Dong Nyoung, Lee, Donghyun, Rim, Hyun Joon, Lee, Deok-Won, Sung, Hak-Joon, Kwon, Il Keun
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Germany Blackwell Publishing Ltd 01.07.2016
Wiley Subscription Services, Inc
Schlagworte:
adamantine
adipose derived stem cell
Animals
beta-cyclodextrin
beta-Cyclodextrins - chemistry
Bone Development - drug effects
bone tissue engineering
Cell Differentiation - drug effects
Durapatite - administration & dosage
Durapatite - chemistry
Gelatin - administration & dosage
Gelatin - chemistry
Gene expression
Humans
Hydroxyapatite
Mineralization
Nanostructured materials
Osteogenesis - drug effects
Polyesters - administration & dosage
Polyesters - chemistry
Rabbits
simvastatin
Simvastatin - administration & dosage
Simvastatin - chemistry
Stem cells
Tissue Engineering
Tissue Scaffolds - chemistry
Wound Healing - drug effects
adipose derived stem cell
simvastatin
beta-cyclodextrin
adamantine
bone tissue engineering
ISSN:1616-5187, 1616-5195, 1616-5195
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Zusammenfassung:Recently, the application of nanostructured materials in the field of tissue engineering has garnered attention to mediate treatment and regeneration of bone defects. In this study, poly(l‐lactic acid) (PLLA)/gelatin (PG) fibrous scaffolds are fabricated and β‐cyclodextrin (βCD) grafted nano‐hydroxyapatite (HAp) is coated onto the fibrous scaffold surface via an interaction between βCD and adamantane. Simvastatin (SIM), which is known to promote osteoblast viability and differentiation, is loaded into the remaining βCD. The specimen morphologies are characterized by scanning electron microscopy. The release profile of SIM from the drug loaded scaffold is also evaluated. In vitro proliferation and osteogenic differentiation of human adipose derived stem cells on SIM/HAp coated PG composite scaffolds is characterized by alkaline phosphatase (ALP) activity, mineralization (Alizarin Red S staining), and real time Polymerase chain reaction (PCR). The scaffolds are then implanted into rabbit calvarial defects and analyzed by microcomputed tomography for bone formation after four and eight weeks. These results demonstrate that SIM loaded PLLA/gelatin/HAp‐(βCD) scaffolds promote significantly higher ALP activity, mineralization, osteogenic gene expression, and bone regeneration than control scaffolds. This suggests the potential application of this material toward bone tissue engineering. β‐Cyclodextrin grafted hydroxyapatite nanoparticles are coated onto the surface of PLLA/gelatin fibrous scaffolds by an inclusion complex system. To provide a favorable microenvironment for bone tissue regeneration, simvastatin, an osteoinductive drug, is loaded into β‐cyclodextrin within the hydroxyapatite nanoparticle.
Bibliographie:Ministry of Education, Science and Technology - No. NRF-2012R1A5A2051388; No. NRF-2010-0019346
ark:/67375/WNG-NTXCVD9Q-M
ArticleID:MABI201500450
istex:3C623D6755AC5B58511FEBDA6A36FFAB900C76A7
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1616-5187
1616-5195
1616-5195
DOI:10.1002/mabi.201500450