Overexpression of a WRKY Transcription Factor TaWRKY2 Enhances Drought Stress Tolerance in Transgenic Wheat

Drought is a major environmental stress that severely restricts plant growth and crop productivity. A previous study showed that from wheat ( ) plays an important role in drought stress tolerance. In the present study, we isolated the promoter of and identified multiple regulatory -elements in the p...

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Vydané v:Frontiers in plant science Ročník 9; s. 997
Hlavní autori: Gao, Huiming, Wang, Yafei, Xu, Ping, Zhang, Zhengbin
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Switzerland Frontiers Media SA 07.08.2018
Frontiers Media S.A
Predmet:
Abiotic stress
Abscisic acid
Cloning
Crop production
Crop yield
Cultivars
Drought
Drought resistance
Environmental stress
Genes
Genetic engineering
Grain
Leaves
Physiology
Plant growth
Plant Science
Seedlings
TaWRKY2
transcription factors
transgenic wheat
Water loss
Wheat
yield
Beijing China
China
transgenic wheat
drought
transcription factors
TaWRKY2
yield
ISSN:1664-462X, 1664-462X
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Shrnutí:Drought is a major environmental stress that severely restricts plant growth and crop productivity. A previous study showed that from wheat ( ) plays an important role in drought stress tolerance. In the present study, we isolated the promoter of and identified multiple regulatory -elements in the promoter region. The activity of the promoter was induced by drought, salt, heat, and abscisic acid (ABA). We also generated -overexpressing transgenic wheat, and found that the transgenic seedlings exhibited significantly enhanced tolerance to drought stress, as evidenced by a higher survival rate and lower water loss rate of detached leaves compared with wild type (WT) plants. In addition, the transgenic lines had higher contents of free proline, soluble sugar, and chlorophyll. During a prolonged period of drought stress before the heading stage, the growth of WT plants was inhibited, whereas the -overexpressing lines progressed to the heading stage. The increased grain yield of the transgenic wheat lines reflected the cumulative effects of longer panicle length, more kernels per spike, and greater aboveground biomass. Our findings show that can enhance drought tolerance and increase grain yield in wheat, thus providing a promising candidate target for improving the drought tolerance of wheat cultivars through genetic engineering.
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Reviewed by: Charu Lata, National Botanical Research Institute (CSIR), India; Lingaraj Sahoo, Indian Institute of Technology Guwahati, India
Edited by: Viswanathan Chinnusamy, Indian Agricultural Research Institute (ICAR), India
This article was submitted to Plant Abiotic Stress, a section of the journal Frontiers in Plant Science
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2018.00997