Nemo: a computational tool for analyzing nematode locomotion

The nematode Caenorhabditis elegans responds to an impressive range of chemical, mechanical and thermal stimuli and is extensively used to investigate the molecular mechanisms that mediate chemosensation, mechanotransduction and thermosensation. The main behavioral output of these responses is manif...

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Vydáno v:BMC neuroscience Ročník 8; číslo 1; s. 86
Hlavní autoři: Tsibidis, George D, Tavernarakis, Nektarios
Médium: Journal Article
Jazyk:angličtina
Vydáno: England BioMed Central Ltd 17.10.2007
BioMed Central
BMC
Témata:
Algorithms
Analysis
Animals
Applications software
Behavior, Animal
Caenorhabditis elegans
Caenorhabditis elegans - physiology
Locomotion - physiology
Methods
Microscopy, Video
Nematoda
Numerical Analysis, Computer-Assisted
Software
United Kingdom
ISSN:1471-2202, 1471-2202
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Shrnutí:The nematode Caenorhabditis elegans responds to an impressive range of chemical, mechanical and thermal stimuli and is extensively used to investigate the molecular mechanisms that mediate chemosensation, mechanotransduction and thermosensation. The main behavioral output of these responses is manifested as alterations in animal locomotion. Monitoring and examination of such alterations requires tools to capture and quantify features of nematode movement. In this paper, we introduce Nemo (nematode movement), a computationally efficient and robust two-dimensional object tracking algorithm for automated detection and analysis of C. elegans locomotion. This algorithm enables precise measurement and feature extraction of nematode movement components. In addition, we develop a Graphical User Interface designed to facilitate processing and interpretation of movement data. While, in this study, we focus on the simple sinusoidal locomotion of C. elegans, our approach can be readily adapted to handle complicated locomotory behaviour patterns by including additional movement characteristics and parameters subject to quantification. Our software tool offers the capacity to extract, analyze and measure nematode locomotion features by processing simple video files. By allowing precise and quantitative assessment of behavioral traits, this tool will assist the genetic dissection and elucidation of the molecular mechanisms underlying specific behavioral responses.
Bibliografie:ObjectType-Article-1
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ISSN:1471-2202
1471-2202
DOI:10.1186/1471-2202-8-86