Nanoscale chemical imaging using tip-enhanced Raman spectroscopy

Confocal and surface-enhanced Raman spectroscopy (SERS) are powerful techniques for molecular characterization; however, they suffer from the drawback of diffraction-limited spatial resolution. Tip-enhanced Raman spectroscopy (TERS) overcomes this limitation and provides chemical information at leng...

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Vydané v:	Nature protocols Ročník 14; číslo 4; s. 1169 - 1193
Hlavní autori:	Kumar, Naresh, Weckhuysen, Bert M, Wain, Andrew J, Pollard, Andrew J
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	England Nature Publishing Group 01.04.2019
Predmet:	Adipocytes - ultrastructure Animals Atomic force microscopy Biological properties Biological samples Cell Line Chemical analysis Data processing Equipment Design - methods Graphene Humans Imaging Mice Microscopy, Atomic Force - instrumentation Microscopy, Atomic Force - methods Molecular Imaging - instrumentation Molecular Imaging - methods Nanotechnology Nanotubes Organic chemistry Polymer films Polymers Probes Raman spectroscopy Self-assembled monolayers Self-assembly Single wall carbon nanotubes Spatial resolution Spectroscopy Spectrum analysis Spectrum Analysis, Raman - instrumentation Spectrum Analysis, Raman - methods Substrates Thin films
ISSN:	1754-2189, 1750-2799, 1750-2799
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Abstract	Confocal and surface-enhanced Raman spectroscopy (SERS) are powerful techniques for molecular characterization; however, they suffer from the drawback of diffraction-limited spatial resolution. Tip-enhanced Raman spectroscopy (TERS) overcomes this limitation and provides chemical information at length scales in the tens of nanometers. In contrast to alternative approaches to nanoscale chemical analysis, TERS is label free, is non-destructive, and can be performed in both air and liquid environments, allowing its use in a diverse range of applications. Atomic force microscopy (AFM)-based TERS is especially versatile, as it can be applied to a broad range of samples on various substrates. Despite its advantages, widespread uptake of this technique for nanoscale chemical imaging has been inhibited by various experimental challenges, such as limited lifetime, and the low stability and yield of TERS probes. This protocol details procedures that will enable researchers to reliably perform TERS imaging using a transmission-mode AFM-TERS configuration on both biological and non-biological samples. The procedure consists of four stages: (i) preparation of plasmonically active TERS probes; (ii) alignment of the TERS system; (iii) experimental procedures for nanoscale imaging using TERS; and (iv) TERS data processing. We provide procedures and example data for a range of different sample types, including polymer thin films, self-assembled monolayers (SAMs) of organic molecules, photocatalyst surfaces, small molecules within biological cells, single-layer graphene and single-walled carbon nanotubes in both air and water. With this protocol, TERS probes can be prepared within ~23 h, and each subsequent TERS experimental procedure requires 3-5 h.
AbstractList	Confocal and surface-enhanced Raman spectroscopy (SERS) are powerful techniques for molecular characterization; however, they suffer from the drawback of diffraction-limited spatial resolution. Tip-enhanced Raman spectroscopy (TERS) overcomes this limitation and provides chemical information at length scales in the tens of nanometers. In contrast to alternative approaches to nanoscale chemical analysis, TERS is label free, is non-destructive, and can be performed in both air and liquid environments, allowing its use in a diverse range of applications. Atomic force microscopy (AFM)-based TERS is especially versatile, as it can be applied to a broad range of samples on various substrates. Despite its advantages, widespread uptake of this technique for nanoscale chemical imaging has been inhibited by various experimental challenges, such as limited lifetime, and the low stability and yield of TERS probes. This protocol details procedures that will enable researchers to reliably perform TERS imaging using a transmission-mode AFM-TERS configuration on both biological and non-biological samples. The procedure consists of four stages: (i) preparation of plasmonically active TERS probes; (ii) alignment of the TERS system; (iii) experimental procedures for nanoscale imaging using TERS; and (iv) TERS data processing. We provide procedures and example data for a range of different sample types, including polymer thin films, self-assembled monolayers (SAMs) of organic molecules, photocatalyst surfaces, small molecules within biological cells, single-layer graphene and single-walled carbon nanotubes in both air and water. With this protocol, TERS probes can be prepared within ~23 h, and each subsequent TERS experimental procedure requires 3–5 h.This protocol describes how to perform nanoscale chemical imaging using tip-enhanced Raman spectroscopy (TERS). The procedure details the preparation of plasmonically active TERS probes, alignment of a TERS system, and various example procedures. Confocal and surface-enhanced Raman spectroscopy (SERS) are powerful techniques for molecular characterization; however, they suffer from the drawback of diffraction-limited spatial resolution. Tip-enhanced Raman spectroscopy (TERS) overcomes this limitation and provides chemical information at length scales in the tens of nanometers. In contrast to alternative approaches to nanoscale chemical analysis, TERS is label free, is non-destructive, and can be performed in both air and liquid environments, allowing its use in a diverse range of applications. Atomic force microscopy (AFM)-based TERS is especially versatile, as it can be applied to a broad range of samples on various substrates. Despite its advantages, widespread uptake of this technique for nanoscale chemical imaging has been inhibited by various experimental challenges, such as limited lifetime, and the low stability and yield of TERS probes. This protocol details procedures that will enable researchers to reliably perform TERS imaging using a transmission-mode AFM-TERS configuration on both biological and non-biological samples. The procedure consists of four stages: (i) preparation of plasmonically active TERS probes; (ii) alignment of the TERS system; (iii) experimental procedures for nanoscale imaging using TERS; and (iv) TERS data processing. We provide procedures and example data for a range of different sample types, including polymer thin films, self-assembled monolayers (SAMs) of organic molecules, photocatalyst surfaces, small molecules within biological cells, single-layer graphene and single-walled carbon nanotubes in both air and water. With this protocol, TERS probes can be prepared within ~23 h, and each subsequent TERS experimental procedure requires 3-5 h. Confocal and surface-enhanced Raman spectroscopy (SERS) are powerful techniques for molecular characterization; however, they suffer from the drawback of diffraction-limited spatial resolution. Tip-enhanced Raman spectroscopy (TERS) overcomes this limitation and provides chemical information at length scales in the tens of nanometers. In contrast to alternative approaches to nanoscale chemical analysis, TERS is label free, is non-destructive, and can be performed in both air and liquid environments, allowing its use in a diverse range of applications. Atomic force microscopy (AFM)-based TERS is especially versatile, as it can be applied to a broad range of samples on various substrates. Despite its advantages, widespread uptake of this technique for nanoscale chemical imaging has been inhibited by various experimental challenges, such as limited lifetime, and the low stability and yield of TERS probes. This protocol details procedures that will enable researchers to reliably perform TERS imaging using a transmission-mode AFM-TERS configuration on both biological and non-biological samples. The procedure consists of four stages: (i) preparation of plasmonically active TERS probes; (ii) alignment of the TERS system; (iii) experimental procedures for nanoscale imaging using TERS; and (iv) TERS data processing. We provide procedures and example data for a range of different sample types, including polymer thin films, self-assembled monolayers (SAMs) of organic molecules, photocatalyst surfaces, small molecules within biological cells, single-layer graphene and single-walled carbon nanotubes in both air and water. With this protocol, TERS probes can be prepared within ~23 h, and each subsequent TERS experimental procedure requires 3-5 h.Confocal and surface-enhanced Raman spectroscopy (SERS) are powerful techniques for molecular characterization; however, they suffer from the drawback of diffraction-limited spatial resolution. Tip-enhanced Raman spectroscopy (TERS) overcomes this limitation and provides chemical information at length scales in the tens of nanometers. In contrast to alternative approaches to nanoscale chemical analysis, TERS is label free, is non-destructive, and can be performed in both air and liquid environments, allowing its use in a diverse range of applications. Atomic force microscopy (AFM)-based TERS is especially versatile, as it can be applied to a broad range of samples on various substrates. Despite its advantages, widespread uptake of this technique for nanoscale chemical imaging has been inhibited by various experimental challenges, such as limited lifetime, and the low stability and yield of TERS probes. This protocol details procedures that will enable researchers to reliably perform TERS imaging using a transmission-mode AFM-TERS configuration on both biological and non-biological samples. The procedure consists of four stages: (i) preparation of plasmonically active TERS probes; (ii) alignment of the TERS system; (iii) experimental procedures for nanoscale imaging using TERS; and (iv) TERS data processing. We provide procedures and example data for a range of different sample types, including polymer thin films, self-assembled monolayers (SAMs) of organic molecules, photocatalyst surfaces, small molecules within biological cells, single-layer graphene and single-walled carbon nanotubes in both air and water. With this protocol, TERS probes can be prepared within ~23 h, and each subsequent TERS experimental procedure requires 3-5 h.
Author	Pollard, Andrew J Kumar, Naresh Wain, Andrew J Weckhuysen, Bert M
Author_xml	– sequence: 1 givenname: Naresh orcidid: 0000-0001-8953-5420 surname: Kumar fullname: Kumar, Naresh organization: Inorganic Chemistry and Catalysis Group, Debye Institute for Nanomaterials Science, Utrecht University, Utrecht, the Netherlands – sequence: 2 givenname: Bert M surname: Weckhuysen fullname: Weckhuysen, Bert M organization: Inorganic Chemistry and Catalysis Group, Debye Institute for Nanomaterials Science, Utrecht University, Utrecht, the Netherlands – sequence: 3 givenname: Andrew J orcidid: 0000-0002-8666-6158 surname: Wain fullname: Wain, Andrew J organization: National Physical Laboratory, Teddington, UK – sequence: 4 givenname: Andrew J orcidid: 0000-0002-6841-2592 surname: Pollard fullname: Pollard, Andrew J email: andrew.pollard@npl.co.uk organization: National Physical Laboratory, Teddington, UK. andrew.pollard@npl.co.uk
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SubjectTerms	Adipocytes - ultrastructure Animals Atomic force microscopy Biological properties Biological samples Cell Line Chemical analysis Data processing Equipment Design - methods Graphene Humans Imaging Mice Microscopy, Atomic Force - instrumentation Microscopy, Atomic Force - methods Molecular Imaging - instrumentation Molecular Imaging - methods Nanotechnology Nanotubes Organic chemistry Polymer films Polymers Probes Raman spectroscopy Self-assembled monolayers Self-assembly Single wall carbon nanotubes Spatial resolution Spectroscopy Spectrum analysis Spectrum Analysis, Raman - instrumentation Spectrum Analysis, Raman - methods Substrates Thin films
Title	Nanoscale chemical imaging using tip-enhanced Raman spectroscopy
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