Effects of naphthalene on gene transcription in Calanus finmarchicus (Crustacea: Copepoda)

The planktonic copepod Calanus finmarchicus is a key species in the Northern Atlantic food web; an oceanic area with extensive oil production. Naphthalene is one of the major constituents of produced water and water soluble fractions of petrogenic oils. This study investigates the effects on gene tr...

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Bibliographic Details
Published in:Aquatic toxicology Vol. 86; no. 2; pp. 157 - 165
Main Authors: Hansen, Bjørn Henrik, Altin, Dag, Vang, Siv-Hege, Nordtug, Trond, Olsen, Anders J.
Format: Journal Article
Language:English
Published: Amsterdam Elsevier B.V 31.01.2008
Elsevier Science
Subjects:
Animal, plant and microbial ecology
Animals
Antioxidant
antioxidant activity
Applied ecology
Biological and medical sciences
Biotransformation
Calanus finmarchicus
Copepoda
Copepoda - drug effects
Crustacea
Detoxification
DNA Primers - chemistry
Ecotoxicology, biological effects of pollution
Enzymes - analysis
Enzymes - biosynthesis
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation - drug effects
gene induction
General aspects
glutathione transferase
Invertebrates
lethal concentration 50
lipid peroxidation
Marine
messenger RNA
metabolic detoxification
naphthalene
Naphthalenes - analysis
Naphthalenes - toxicity
Organic pollution
Petroleum
plankton
Polymerase Chain Reaction - veterinary
Protein turnover
Proteins - analysis
RNA, Messenger - analysis
Time Factors
toxicity
transcriptional activation
Water Pollutants, Chemical - toxicity
water pollution
AN, Atlantic
Calanus finmarchicus
Organic pollution
Biotransformation
Detoxification
Antioxidant
Protein turnover
Petroleum
Ecotoxicology
Transcription
Protein
Crustacea
Aquatic environment
Pollution
Naphthalene
Arthropoda
Copepoda
Aromatic compound
Invertebrata
ISSN:0166-445X, 1879-1514
Online Access:Get full text
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Summary:The planktonic copepod Calanus finmarchicus is a key species in the Northern Atlantic food web; an oceanic area with extensive oil production. Naphthalene is one of the major constituents of produced water and water soluble fractions of petrogenic oils. This study investigates the effects on gene transcription of a short term exposure to naphthalene at levels well below LC 50 concentrations. This was done in order to establish a molecular basis of naphthalene toxicity in a species which has previously been subject only to very limited studies at the molecular level. Naphthalene exposure to C. finmarchicus was found to cause glutathione S-transferase (GST) induction, indicating lipid peroxidation as the major mode of naphthalene toxicity. There is no clear evidence that the putative cytochrome P450 enzymes CYP1A2 and CYP330A1 mRNAs are parts of a detoxification enzyme system. Instead, an observed decrease in CYP330A1 mRNA levels at the highest naphthalene exposure concentration may indicate an effect on ecdysteroidogenesis. Only the lowest naphthalene concentration lead to increased mRNA levels of antioxidants SOD and CAT, indicating no clear evidence for general cellular oxidative stress following exposure. Small and insignificant changes in the HSP-70, HSP-90 and ubiquitin mRNA levels indicate a small degree of protein damage owing to naphthalene exposure. The established culture of C. finmarchicus at the SINTEF/NTNU Sealab, and the use of gene transcription analyses provide excellent tools for improving the understanding of biochemical mechanisms involved in the defense against environmental impacts and the molecular modes of toxicity in this species.
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ISSN:0166-445X
1879-1514
DOI:10.1016/j.aquatox.2007.10.009