WAPL-mediated removal of cohesin protects against segregation errors and aneuploidy

The classical X shape of mitotic human chromosomes is the consequence of two distinct waves of cohesin removal. First, during prophase and prometaphase, the bulk of cohesin is driven from chromosome arms by the cohesin antagonist WAPL. This arm-specific cohesin removal is referred to as the prophase...

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Vydáno v:Current biology Ročník 23; číslo 20; s. 2071
Hlavní autoři: Haarhuis, Judith H I, Elbatsh, Ahmed M O, van den Broek, Bram, Camps, Daniel, Erkan, Hasan, Jalink, Kees, Medema, René H, Rowland, Benjamin D
Médium: Journal Article
Jazyk:angličtina
Vydáno: England 21.10.2013
Témata:
Aneuploidy
Aurora Kinase B - genetics
Aurora Kinase B - metabolism
Carrier Proteins - genetics
Carrier Proteins - metabolism
Cell Cycle Proteins - genetics
Cell Cycle Proteins - metabolism
Cell Line
Centromere - metabolism
Chromatids - metabolism
Chromosomal Proteins, Non-Histone - genetics
Chromosomal Proteins, Non-Histone - metabolism
Chromosome Segregation
Chromosomes, Human - metabolism
Cohesins
Humans
Microscopy, Phase-Contrast
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Prophase
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins - metabolism
Retinal Pigment Epithelium
ISSN:1879-0445, 1879-0445
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Shrnutí:The classical X shape of mitotic human chromosomes is the consequence of two distinct waves of cohesin removal. First, during prophase and prometaphase, the bulk of cohesin is driven from chromosome arms by the cohesin antagonist WAPL. This arm-specific cohesin removal is referred to as the prophase pathway [1-4]. The subsequent cleavage of the remaining centromeric cohesin by Separase is known to be the trigger for anaphase onset [5-7]. Remarkably the biological purpose of the prophase pathway is unknown. We find that this pathway is essential for two key mitotic processes. First, it is important to focus Aurora B at centromeres to allow efficient correction of erroneous microtubule-kinetochore attachments. In addition, it is required to facilitate the timely decatenation of sister chromatids. As a consequence, WAPL-depleted cells undergo anaphase with segregation errors, including both lagging chromosomes and catenanes, resulting in micronuclei and DNA damage. Stable WAPL depletion arrests cells in a p53-dependent manner but causes p53-deficient cells to become highly aneuploid. Our data show that the WAPL-dependent prophase pathway is essential for proper chromosome segregation and is crucial to maintain genomic integrity.
Bibliografie:ObjectType-Article-1
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ISSN:1879-0445
1879-0445
DOI:10.1016/j.cub.2013.09.003