Cells susceptible to epithelial-mesenchymal transition are enriched in stem-like side population cells from prostate cancer

Accumulating evidence suggests that epithelial-mesenchymal transition (EMT) acts as an important factor for the promotion of tumor progression. Strategies for suppressing EMT remain the subject of ongoing research. In the present study, fluorescence-activated cell sorting (FACS) was used to isolate...

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Bibliographic Details
Published in:Oncology reports Vol. 31; no. 2; pp. 874 - 884
Main Authors: LUO, YONG, CUI, XINHAO, ZHAO, JIAHUI, HAN, YILI, LI, MINGCHUAN, LIN, YUNHUA, JIANG, YONGGUANG, LAN, LING
Format: Journal Article
Language:English
Published: Greece D.A. Spandidos 01.02.2014
Spandidos Publications
Spandidos Publications UK Ltd
Subjects:
Actins - biosynthesis
Analysis
Animals
beta Catenin - biosynthesis
Biomarkers, Tumor - biosynthesis
Bone Neoplasms - secondary
Cadherins - biosynthesis
cancer stem cells
Care and treatment
Cell Line, Tumor
Cell Proliferation
Cell Transformation, Neoplastic - genetics
Chemotherapy
China
Cloning
Comparative analysis
Development and progression
Epithelial-Mesenchymal Transition
Fluorescence
Gene Expression Regulation, Neoplastic
Genotype & phenotype
Health aspects
heterogeneity
Humans
hypoxia inducible factor-1α
Hypoxia-Inducible Factor 1, alpha Subunit - genetics
Male
Matrix Metalloproteinase 2 - biosynthesis
Medical research
Membranes
Metastasis
Mice
Mice, Nude
Mortality
Neoplasm Invasiveness
Neoplasm Transplantation
Neoplastic Stem Cells - cytology
Neoplastic Stem Cells - pathology
Population
Prostate cancer
Prostatic Neoplasms - genetics
Prostatic Neoplasms - pathology
Protein expression
Proteins
side population cells
Side-Population Cells - cytology
Side-Population Cells - pathology
Stem cells
Studies
Transfection
Transplantation, Heterologous
Tumors
Vimentin - biosynthesis
China
United States > US
ISSN:1021-335X, 1791-2431, 1791-2431
Online Access:Get full text
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Summary:Accumulating evidence suggests that epithelial-mesenchymal transition (EMT) acts as an important factor for the promotion of tumor progression. Strategies for suppressing EMT remain the subject of ongoing research. In the present study, fluorescence-activated cell sorting (FACS) was used to isolate side population (SP) cells from human prostate cancer (PCa) cell lines and xenograft tissues. After identifying their molecular and functional stem-like characteristics, stem-like SP cells from a cell line and from xenograft tissue were transfected with hypoxia inducible factor-1α (HIF-1α). The potential of the prostate stem-like SP cells to undergo EMT was compared with that in their bulk counterparts after HIF-1α introduction. Stem-like SP cells acquired more complete EMT molecular features and exhibited stronger aggressive capability than the homologous bulk population cells both in vitro (proliferation and invasion) and in vivo (tumorigenesis and metastasis formation). We, therefore, concluded that EMT is closely associated with tumor heterogeneity, and that PCa cells susceptible to EMT are enriched in stem-like SP cells. These findings disclose a new approach, targeting the cellular basis of the EMT process that may help to identify effective and accurate methods for suppressing tumor growth and preventing distant dissemination.
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ISSN:1021-335X
1791-2431
1791-2431
DOI:10.3892/or.2013.2905