Overexpression of miR-422a inhibits cell proliferation and invasion, and enhances chemosensitivity in osteosarcoma cells

Osteosarcoma (OS) is an aggressive malignant tumor that is mesenchymal in origin with a very low 5-year survival rate, particularly in the patients with locally advanced or metastatic tumors and recurrent disease. MicroRNAs (miRNAs) play a critical role in essential biological processes as cellular...

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Bibliographic Details
Published in:Oncology reports Vol. 36; no. 6; pp. 3371 - 3378
Main Authors: Liu, Mingjiang, Xiusheng, He, Xiao, Xiangjun, Wang, Yichun
Format: Journal Article
Language:English
Published: Greece D.A. Spandidos 01.12.2016
Spandidos Publications
Spandidos Publications UK Ltd
Subjects:
3' Untranslated Regions
Antineoplastic Agents - pharmacology
Apoptosis
Base Sequence
Binding Sites
Bone cancer
Bone Neoplasms - drug therapy
Bone Neoplasms - genetics
Bone Neoplasms - metabolism
Cancer therapies
Care and treatment
Cell growth
Cell Line, Tumor
Cell Movement
Cell Proliferation
chemosensitivity
Chemotherapy
Cisplatin - pharmacology
Colorectal cancer
Development and progression
Gene Expression
Gene Expression Regulation, Neoplastic
Genetic aspects
Growth factors
Health aspects
Humans
Innovations
Kinases
Medical prognosis
Membranes
Metastasis
MicroRNA
MicroRNAs - genetics
MicroRNAs - metabolism
Molecular targeted therapy
Neoplasm Invasiveness
Osteosarcoma
Osteosarcoma - drug therapy
Osteosarcoma - genetics
Osteosarcoma - metabolism
Paclitaxel - pharmacology
Pathogenesis
Proteins
RNA Interference
Sarcoma
smad
Transforming Growth Factor beta2 - genetics
Transforming Growth Factor beta2 - metabolism
transforming growth factorβ2
United States > US
China
ISSN:1021-335X, 1791-2431, 1791-2431
Online Access:Get full text
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Description
Summary:Osteosarcoma (OS) is an aggressive malignant tumor that is mesenchymal in origin with a very low 5-year survival rate, particularly in the patients with locally advanced or metastatic tumors and recurrent disease. MicroRNAs (miRNAs) play a critical role in essential biological processes as cellular proliferation, differentiation and apoptosis in normal or cancer cells, including OS cells. In the present study, we aimed to investigate the role of miR-422a in OS. We demonstrated that miR-422a expression was significantly downregulated in OS tissues and cell lines compared with the normal controls. In addition, overexpression of miR-422a was able to inhibit cell proliferation and the ability of invasion, and enhance paclitaxel and cisplatin-mediated apoptosis in OS cells. Inversely, downregulation of miR-422a exhibited an opposite role. We further demonstrated that miR-422a directly targeted TGFβ2 and regulated its expression and the activation of downstream molecules, smad2 and smad3 in OS cells. Thus, miR-422a/TGFβ2/smad axis may be a potential target for OS treatment.
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ISSN:1021-335X
1791-2431
1791-2431
DOI:10.3892/or.2016.5182