Overexpression of miR-422a inhibits cell proliferation and invasion, and enhances chemosensitivity in osteosarcoma cells

Osteosarcoma (OS) is an aggressive malignant tumor that is mesenchymal in origin with a very low 5-year survival rate, particularly in the patients with locally advanced or metastatic tumors and recurrent disease. MicroRNAs (miRNAs) play a critical role in essential biological processes as cellular...

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Vydané v:	Oncology reports Ročník 36; číslo 6; s. 3371 - 3378
Hlavní autori:	Liu, Mingjiang, Xiusheng, He, Xiao, Xiangjun, Wang, Yichun
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	Greece D.A. Spandidos 01.12.2016 Spandidos Publications Spandidos Publications UK Ltd
Predmet:	3' Untranslated Regions Antineoplastic Agents - pharmacology Apoptosis Base Sequence Binding Sites Bone cancer Bone Neoplasms - drug therapy Bone Neoplasms - genetics Bone Neoplasms - metabolism Cancer therapies Care and treatment Cell growth Cell Line, Tumor Cell Movement Cell Proliferation chemosensitivity Chemotherapy Cisplatin - pharmacology Colorectal cancer Development and progression Gene Expression Gene Expression Regulation, Neoplastic Genetic aspects Growth factors Health aspects Humans Innovations Kinases Medical prognosis Membranes Metastasis MicroRNA MicroRNAs - genetics MicroRNAs - metabolism Molecular targeted therapy Neoplasm Invasiveness Osteosarcoma Osteosarcoma - drug therapy Osteosarcoma - genetics Osteosarcoma - metabolism Paclitaxel - pharmacology Pathogenesis Proteins RNA Interference Sarcoma smad Transforming Growth Factor beta2 - genetics Transforming Growth Factor beta2 - metabolism transforming growth factorβ2 United States > US China
ISSN:	1021-335X, 1791-2431, 1791-2431
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Popis
Shrnutí:	Osteosarcoma (OS) is an aggressive malignant tumor that is mesenchymal in origin with a very low 5-year survival rate, particularly in the patients with locally advanced or metastatic tumors and recurrent disease. MicroRNAs (miRNAs) play a critical role in essential biological processes as cellular proliferation, differentiation and apoptosis in normal or cancer cells, including OS cells. In the present study, we aimed to investigate the role of miR-422a in OS. We demonstrated that miR-422a expression was significantly downregulated in OS tissues and cell lines compared with the normal controls. In addition, overexpression of miR-422a was able to inhibit cell proliferation and the ability of invasion, and enhance paclitaxel and cisplatin-mediated apoptosis in OS cells. Inversely, downregulation of miR-422a exhibited an opposite role. We further demonstrated that miR-422a directly targeted TGFβ2 and regulated its expression and the activation of downstream molecules, smad2 and smad3 in OS cells. Thus, miR-422a/TGFβ2/smad axis may be a potential target for OS treatment.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	1021-335X 1791-2431 1791-2431
DOI:	10.3892/or.2016.5182