LRRK2 is expressed in areas affected by Parkinson's disease in the adult mouse brain

The leucine‐rich repeat kinase 2 (LRRK2) gene was recently found to have multiple mutations that are causative for autosomal dominant inherited Parkinson's disease (PD). Previously, we used Northern blot analysis to show that this gene was expressed in the cerebellum, cerebral cortex, medulla,...

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Vydané v:	The European journal of neuroscience Ročník 23; číslo 3; s. 659 - 666
Hlavní autori:	Simón-Sánchez, Javier, Herranz-Pérez, Vicente, Olucha-Bordonau, Francisco, Pérez-Tur, Jordi
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	Oxford, UK Blackwell Publishing Ltd 01.02.2006
Predmet:	Animals Brain - metabolism Brain - pathology Brain Mapping dardarin Disease Models, Animal expression Gene Expression - physiology In Situ Hybridization - methods Leucine-Rich Repeat Serine-Threonine Protein Kinase-2 Male Mice mousebrain Parkinson Disease - metabolism Parkinson Disease - pathology Parkinson's disease Protein-Serine-Threonine Kinases - genetics Protein-Serine-Threonine Kinases - metabolism RNA, Messenger - metabolism
ISSN:	0953-816X, 1460-9568
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Shrnutí:	The leucine‐rich repeat kinase 2 (LRRK2) gene was recently found to have multiple mutations that are causative for autosomal dominant inherited Parkinson's disease (PD). Previously, we used Northern blot analysis to show that this gene was expressed in the cerebellum, cerebral cortex, medulla, spinal cord, occipital pole, frontal lobe, temporal lobe and caudate putamen. However, a more comprehensive map of LRRK2 mRNA localization in the central nervous system is still lacking. In this study we have mapped the distribution of the mRNA encoding for LRRK2 using nonradioactive in situ hybridization. We detected a moderate expression of this PD‐related gene throughout the adult B2B6 mouse brain. A stronger hybridization signal was observed in deep cerebral cortex layers, superficial cingulate cortex layers, the piriform cortex, hippocampal formation, caudate putamen, substantia nigra, the basolateral and basomedial anterior amygdala nuclei, reticular thalamic nucleus and also in the cerebellar granular cell layer. Given that LRRK2 mRNA is highly enriched in motor systems and also is expressed in other systems, we may conclude that mutations in LRRK2 may affect several motor and nonmotor structures that may play an important role in the development of PD.
Bibliografia:	istex:76D9E589C318BE7BCC628529E9CE34BAC2C202FE ark:/67375/WNG-KQRSTSL0-1 ArticleID:EJN4616 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0953-816X 1460-9568
DOI:	10.1111/j.1460-9568.2006.04616.x