Organometallic and radical intermediates reveal mechanism of diphthamide biosynthesis

Diphthamide biosynthesis involves a carbon-carbon bond-forming reaction catalyzed by a radical S-adenosylmethionine (SAM) enzyme that cleaves a carbon-sulfur (C-S) bond in SAM to generate a 3-amino-3-carboxypropyl (ACP) radical. Using rapid freezing, we have captured an organometallic intermediate w...

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Bibliographic Details

Published in:	Science (American Association for the Advancement of Science) Vol. 359; no. 6381; p. 1247
Main Authors:	Dong, Min, Kathiresan, Venkatesan, Fenwick, Michael K, Torelli, Andrew T, Zhang, Yang, Caranto, Jonathan D, Dzikovski, Boris, Sharma, Ajay, Lancaster, Kyle M, Freed, Jack H, Ealick, Steven E, Hoffman, Brian M, Lin, Hening
Format:	Journal Article
Language:	English
Published:	United States 16.03.2018
Subjects:	Archaeal Proteins - chemistry Carbon - chemistry Crystallography, X-Ray Histidine - analogs & derivatives Histidine - biosynthesis Iron - chemistry Iron-Sulfur Proteins - chemistry Organometallic Compounds - chemistry Pyrococcus horikoshii - enzymology S-Adenosylmethionine - chemistry
ISSN:	1095-9203, 1095-9203
Online Access:	Get more information
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